期刊
EMBO JOURNAL
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.15252/embj.2022112814
关键词
ATG proteins; autophagy; stepwise assembly; TOR; Ypt1
In this study, the direct interaction between Rab GTPase Ypt1/Rab1 and Atg23 and Atg17 was found to be crucial for the stepwise assembly of autophagy initiation complexes. Additionally, the phosphorylation of Ypt1 by TOR was shown to regulate the assembly of ATG proteins during autophagy initiation. Therefore, TOR-mediated Ypt1 is proposed as a multifunctional assembly factor that controls autophagy initiation by regulating the stepwise assembly of ATG proteins.
The regulation of autophagy initiation is a key step in autophagosome biogenesis. However, our understanding of the molecular mechanisms underlying the stepwise assembly of ATG proteins during this process remains incomplete. The Rab GTPase Ypt1/Rab1 is recognized as an essential autophagy regulator. Here, we identify Atg23 and Atg17 as binding partners of Ypt1, with their direct interaction proving crucial for the stepwise assembly of autophagy initiation complexes. Disruption of Ypt1-Atg23 binding results in significantly reduced Atg9 interactions with Atg11, Atg13, and Atg17, thus preventing the recruitment of Atg9 vesicles to the phagophore assembly site (PAS). Likewise, Ypt1-Atg17 binding contributes to the PAS recruitment of Ypt1 and Atg1. Importantly, we found that Ypt1 is phosphorylated by TOR at the Ser174 residue. Converting this residue to alanine blocks Ypt1 phosphorylation by TOR and enhances autophagy. Conversely, the Ypt1S174D phosphorylation mimic impairs both PAS recruitment and activation of Atg1, thus inhibiting subsequent autophagy. Thus, we propose TOR-mediated Ypt1 as a multifunctional assembly factor that controls autophagy initiation via its regulation of the stepwise assembly of ATG proteins.
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