期刊
ELECTROPHORESIS
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/elps.202300059
关键词
body fluid identification; miRNA; mRNA; reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Inferring the tissue origin types of forensic-relevant body fluids is crucial for reconstructing a crime scene, but accurately identifying different kinds of body fluids remains challenging. This study developed a combined mRNA and miRNA system and applied it to identify five common types of body fluids, demonstrating its advantages in human body fluid identification, especially for mixed samples.
Correctly inferring the tissue origin types of forensic-relevant body fluids left at a crime scene is beneficial for reconstructing a crime scene. However, it is still a challenge to accurately identify different kinds of body fluids at a crime scene. Shorter sequence length and anti-degradation microRNA (miRNA) can be used to infer the tissue sources of biological fluid traces, but a limited number of miRNAs are tissue specific. The application of messenger RNA (mRNA) has been confirmed by different studies based on its high tissue specificity. According to the differential expression features of mRNA or miRNA in forensically relevant body fluids, this study developed a simultaneously reversed mRNA and miRNA system and then used these two types of RNAs for the determinations of five common kinds of body fluids. Compared with previously reported single kind of mRNA or miRNA assay, the combined mRNA and miRNA system showed good advantages for human body fluid identifications, especially it could be applied in mixed samples. In conclusion, the obtained results indicated that this combined mRNA and miRNA system might provide a scientific and accurate reference for body fluid identifications.
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