☆ 4.5 Review

Microfluidic characterization of single-cell biophysical properties and the applications in cancer diagnosis

ELECTROPHORESIS (2023)

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Article Engineering, Biomedical

Extensional-Flow Impedance Cytometer for Contactless and Optics-Free Erythrocyte Deformability Analysis

Riccardo Reale et al.

Summary: In this study, a novel approach based on a microfluidic system was proposed to induce erythrocyte deformation using a hyperbolic microchannel and evaluate the deformed cell shape using coplanar electrodes. The method achieved RBC deformation and quantification of cell shape using an electrical anisotropy index, with a throughput of 300 cell/s. This contactless and optics-free approach holds promises for fast and low-cost erythrocyte deformability assays, particularly in point-of-care and resource-limited settings.

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING (2023)

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Article Chemistry, Analytical

Machine learning-based impedance system for real-time recognition of antibiotic-susceptible bacteria with parallel cytometry

Tao Tang et al.

Summary: Impedance cytometry combined with machine learning is used to analyze the response of bacterial single cells to antibiotic treatment in real time, with a high throughput of over one thousand cells per minute. This system provides an online training method on reference particles to distinguish between different types of particles and accurately identify susceptible cells. The results demonstrate the capability of real-time characterization and recognition of individual cells, providing a convenient and efficient method for antibiotic susceptibility testing.

SENSORS AND ACTUATORS B-CHEMICAL (2023)

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Article Biophysics

Machine learning empowered multi-stress level electromechanical phenotyping for high-dimensional single cell analysis

Minhui Liang et al.

Summary: Microfluidics provides a powerful platform for biological analysis by precisely manipulating fluids and microparticles. The imaging and impedance cell analyzer (IM2Cell) introduced here combines single cell level impedance analysis and hydrodynamic mechanical phenotyping, demonstrating multi-stress level mechanical phenotyping capabilities. IM2Cell can characterize cell diameter, deformability responses, and electrical properties, providing high-dimensional information about subcellular components. It has been validated for different cell lines and shows potential for deformability studies of PBMC subpopulations.

BIOSENSORS & BIOELECTRONICS (2023)

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Review Oncology

Biophysical determinants of cancer organotropism

Udochi F. Azubuike et al.

Summary: Metastasis, the spread of cancer cells to distant organs, is the leading cause of cancer-related deaths. Two hypotheses, the 'seed/soil' hypothesis and the 'mechanical' hypothesis, have been proposed to explain organ-specific metastasis. The 'seed/soil' hypothesis suggests that tumor cells with specific properties and compatibility with the stromal environment in distant sites can colonize and grow, while the 'mechanical' hypothesis proposes that non-random seeding is driven by circulation patterns and vascular networks. Recent studies have incorporated mechanobiology concepts to provide additional insights into the mechanisms that regulate organ selectivity during metastatic outgrowth, particularly focusing on the role of endothelial cells.

TRENDS IN CANCER (2023)

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Article Chemistry, Multidisciplinary

Selective Single-Cell Sorting Using a Multisectorial Electroactive Nanowell Platform

Lukas Menze et al.

Summary: In this paper, a microfluidic platform is introduced to overcome the limitation of cell sorters and achieve selective single-cell sorting by capturing a large number of different cell types. The platform demonstrates high accuracy, versatility, and simplicity, making it suitable for reliable sorting of varying complex samples in clinical environments.

ACS NANO (2022)

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Article Chemistry, Analytical

Modified Red Blood Cells as Multimodal Standards for Benchmarking Single-Cell Cytometry and Separation Based on Electrical Physiology

Armita Salahi et al.

Summary: This study demonstrates the use of red blood cells (RBCs) as multimodal standard particles to sensitively identify cells and compute biophysical information based on modified subcellular electrophysiology and associated fluorescence levels.

ANALYTICAL CHEMISTRY (2022)

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Article Biochemical Research Methods

Robotic automation of droplet microfluidics

Tuan M. Tran et al.

Summary: This study demonstrates the automation of droplet microfluidics using commercial fluid-handling robotics, which speeds up the integration of these methods into biological workflows by utilizing the robot's capabilities such as thermal control, incubation, and plate scanning.

BIOMICROFLUIDICS (2022)

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Article Engineering, Electrical & Electronic

Optimisation of an Electrical Impedance Sensor for Use in Microfluidic Chip Electrophoresis

Martin Hantschke et al.

Summary: This paper presents a novel approach to optimize the performance of tetrapolar electrical impedance measurement (TEIM) sensors in microfluidic chip electrophoresis (ME) applications. The study reveals the relationship between sensor, channel and sample parameters, and demonstrates that the sensor performance can be improved through appropriate design.

IEEE SENSORS JOURNAL (2022)

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Article Biochemical Research Methods

Dual-frequency impedance assays for intracellular components in microalgal cells

Tao Tang et al.

Summary: This study presents a label-free and cost-effective method for analyzing microalgal single-cells at subcellular resolution using impedance cytometry. It demonstrates the relationships between impedance signals and submicron intracellular components for the first time. By tracking changes in biophysical properties through electrical diameter and morphology metrics, the study provides insights into how submicron intracellular components and cell morphology affect impedance signals.

LAB ON A CHIP (2022)

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Article Biochemical Research Methods

Neural network-enhanced real-time impedance flow cytometry for single-cell intrinsic characterization

Yongxiang Feng et al.

Summary: The study demonstrates the use of neural network technology to achieve real-time characterization of cellular intrinsic properties and classification based on intrinsic parameters, significantly improving calculation speed. Experimental results show a high accuracy in cell type classification and minimal impact on cell properties from IFC testing.

LAB ON A CHIP (2022)

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Article Biochemical Research Methods

Size and density measurements of single sickle red blood cells using microfluidic magnetic levitation

Utku Goreke et al.

Summary: This study developed a magnetic levitation-based assay for rapid and accurate analysis of density changes in red blood cells during disease states. By measuring the density of individual red blood cells, researchers identified different sub-populations of cells in blood samples from individuals with sickle cell disease, providing a method for further investigating blood cell disorders and the effects of therapies.

LAB ON A CHIP (2022)

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Review Chemistry, Multidisciplinary

Physical Cytometry: Detecting Mass-Related Properties of Single Cells

Yuliang Zhao et al.

Summary: This review discusses the current developments of physical cytometry for quantifying mass-related physical properties of single cells, including the working principle, applications, and unique merits. The review compares these measurement methods in terms of throughput, content, invasiveness, compatibility, and precision. Additionally, it discusses the typical applications of these methods in pathological diagnosis, drug efficacy evaluation, disease treatment, and other related fields.

ACS SENSORS (2022)

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Article Biochemical Research Methods

Interpretation of cell mechanical experiments in microfluidic systems depend on the choice of cellular shape descriptors

Bob Fregin et al.

Summary: The capability to parameterize shapes is important in biomechanics for identifying cells, tracking their motion, and quantifying deformation. This study compared nine different shape descriptors to characterize the deformation of suspended cells. The researchers found that high-throughput viscoelastic measurements of cells in suspension can be performed on an ensemble scale, and different shape descriptors have varying effects on cell deformation evaluation.

BIOMICROFLUIDICS (2022)

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Article Engineering, Electrical & Electronic

Development of Microfluidic System Enabling High-Throughput Characterization of Multiple Biophysical Parameters of Single Cells

Ke Wang et al.

Summary: This article develops a microfluidic platform to characterize the biomechanical and bioelectrical parameters of individual cells simultaneously. It quantifies the inherent biophysical parameters of various cell types and improves the classification success rates using these parameters.

IEEE TRANSACTIONS ON ELECTRON DEVICES (2022)

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Article Physics, Applied

Effect of the mechanical properties of the cell membrane on the transition energy barrier of electroporation

Yan Mi et al.

Summary: The mechanical properties of the cell membrane have been found to affect the transition energy of electroporation, as demonstrated in this study. Both modeling and experimental approaches were used to analyze the impact and it was shown that the elastic strain energy of the cell membrane increases the energy barrier of pore formation, hindering the process.

JOURNAL OF APPLIED PHYSICS (2022)

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Article Biophysics

Cellular biomechanics: Fluid-structure interaction or structural simulation?

L. Papadakis et al.

Summary: This article discusses how cells respond to their mechanical environment and the application of numerical modeling in studying cellular mechanotransduction. The study finds that the consideration of continuum mechanics is a limitation in current models, and that fluid-structure interaction analysis provides a more realistic framework for understanding cellular biomechanics. The results show that FSI analysis stimulates the cell nucleus more effectively and captures more realistic intracellular loading patterns compared to FE modeling.

JOURNAL OF BIOMECHANICS (2022)

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Review Cell Biology

Cell cycle control in cancer

Helen K. Matthews et al.

Summary: This review highlights the importance of cell cycle regulation in preventing genetic errors and cell proliferation, and how disruption of these mechanisms is associated with cancer development. Cancer arises from continuous cell division and evasion of cell cycle control mechanisms.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2022)

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Article Chemistry, Multidisciplinary

Microfluidic Impedance-Deformability Cytometry for Label-Free Single Neutrophil Mechanophenotyping

Chayakorn Petchakup et al.

Summary: This study reports an optics-free impedance-deformability cytometer for multiparametric single cell mechanophenotyping. By profiling human neutrophils, distinct differences in multimodal impedance signatures under various biochemical stimulations were observed.

SMALL (2022)

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Review Biotechnology & Applied Microbiology

Application of Microfluidics in Detection of Circulating Tumor Cells

Can Li et al.

Summary: This paper reviews the recent research progress of microfluidic technology in CTCs separation and detection, discusses the potential application of microfluidic technology in the treatment of tumor metastasis, and briefly explores the development prospect of microfluidic biosensors.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Article Chemistry, Physical

Impedance-Enabled Camera-Free Intrinsic Mechanical Cytometry

Yongxiang Feng et al.

Summary: CFIMC is a camera-free technique that allows real-time measurement of mechanical properties of single cells. It has high throughput and can reveal mechanical differences among different types of cells.

SMALL METHODS (2022)

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Article Multidisciplinary Sciences

Automated measurement of cell mechanical properties using an integrated dielectrophoretic microfluidic device

Hao Yang et al.

Summary: Cell mechanics is closely related to cellular functions and can serve as an effective biomarker for disease onset and progression. However, current techniques for measuring cell mechanical properties have limited data and biological significance. This study proposes a dielectrophoresis-based solution that combines a microfluidic device and an automatic control scheme to automatically obtain cell mechanical data. Experiments demonstrate the automation capability of this device.

ISCIENCE (2022)

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Article Engineering, Biomedical

Measurement of dielectric properties of cells at single-cell resolution using electrorotation

Yuwen Li et al.

Summary: This paper presents a method for measuring the dielectric properties of cells, especially lipid-producing microalgae, at high frequency and single-cell resolution. By integrating electrorotation and negative dielectrophoresis methods, the dielectric properties of cells, including membrane capacitance and cytoplasm conductivity, can be obtained. This method can be used in various applications, such as screening microalgae, separating cells with different dielectric properties, identifying different cell types, and conducting basic biophysical analyses.

BIOMEDICAL MICRODEVICES (2022)

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Article Biophysics

Single-cell assessment of the modulation of macrophage activation by ex vivo intervertebral discs using impedance cytometry

Armita Salahi et al.

Summary: Measurement and modulation of macrophage activation is crucial for addressing chronic back pain and immune rejection. Single-cell methods are needed to quantify the heterogeneity of macrophage activation phenotypes. This study presents a high throughput single-cell impedance cytometry for accurately measuring macrophage activation in a dose and duration dependent manner, without relying on specific signaling pathways.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Multidisciplinary Sciences

High-resolution mass measurements of single budding yeast reveal linear growth segments

Andreas P. Cuny et al.

Summary: The regulation of cell growth is of great significance in physiology, biotechnology, and medicine. However, monitoring the mass and growth of individual cells with high resolution is challenging. In this study, the authors modified a cell balance technique to monitor the proliferation of single yeast cells in real-time. They found that the mass of single yeast cells increases in linear segments of constant growth rates. This technology has the potential to directly and accurately monitor the growth of single cells throughout their cycle.

NATURE COMMUNICATIONS (2022)

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Article Engineering, Biomedical

A Systematic Study of Size Correlation and Young's Modulus Sensitivity for Cellular Mechanical Phenotyping by Microfluidic Approaches

Minhui Liang et al.

Summary: Cellular mechanical properties play a significant role in cell state and health. Microfluidic mechanical phenotyping methods are promising tools that can address the limitations of traditional approaches. This study comprehensively compares two types of microfluidic cellular mechanical phenotyping methods and provides important findings.

ADVANCED HEALTHCARE MATERIALS (2022)

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Article Chemistry, Multidisciplinary

Universal and Sensitive Drug Assessment Biosensing Platform Using Optimal Mechanical Beating Detection of Single Cardiomyocyte

Dongxin Xu et al.

Summary: The preclinical assessment of efficacy and safety is crucial in cardiovascular drug development to ensure effective prevention and treatment of cardiovascular disease. A biosensing platform involving a multichannel electrode array device and a universal mechanical beating detection system has been proposed, which can detect subtle changes in mechanical beating signals induced by cardiovascular drugs and demonstrate high performance in pharmacological assessment.

ACS NANO (2022)

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Article Engineering, Biomedical

Human cancer cells generate spontaneous calcium transients and intercellular waves that modulate tumor growth

Chenyu Liang et al.

Summary: This study reveals that non-excitable cancer cells, such as human colon and prostate cancer cells, can spontaneously initiate and spread intercellular calcium waves, which promote tumor growth. The release of calcium from the endoplasmic reticulum through the inositol-trisphosphate-receptor pathway is identified as a major cause for the initiation of spontaneous calcium transients. Additionally, the spatial-temporal characteristics of calcium dynamics can be modulated by the mechanical stiffness of the culture substrates.

BIOMATERIALS (2022)

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Article Chemistry, Multidisciplinary

Parallel Impedance Cytometry for Real-Time Screening of Bacterial Single Cells from Nano- to Microscale

Tao Tang et al.

Summary: Parallel impedance cytometry is a novel system for calibration and analysis of impedance signals. It allows simultaneous detection of reference and target particles and facilitates efficient measurement and calibration of impedances of various particles or cells.

ACS SENSORS (2022)

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Article Biochemical Research Methods

Cell deformability heterogeneity recognition by unsupervised machine learning from in-flow motion parameters

Maria Isabella Maremonti et al.

Summary: The study introduces a new method for recognizing cell deformability heterogeneity through the measurement of flow motion parameters, which allows for direct assessment of the rheological/mechanical properties of cells.

LAB ON A CHIP (2022)

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Article Chemistry, Analytical

A high-throughput microfluidic device inspired by the Wheatstone bridge principle for characterizing the mechanical properties of single cells

Siyu Hu et al.

Summary: This study developed a high-throughput microfluidic device based on the Wheatstone bridge principle for characterizing the mechanical properties of single cells. The results showed that the input flow rate had little effect on measuring the mechanical properties of the cells, while the ratio of cell radius to effective constriction radius was different.

ANALYTICAL METHODS (2022)

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Review Chemistry, Analytical

Microfluidic flow cytometry for blood-based biomarker analysis

Yuxin Zhang et al.

Summary: Microfluidic flow cytometry (MFCM), combining flow cytometry and microfluidics, provides a powerful tool for single-cell analysis and sorting of various biological samples. Recent progress and achievements have been made in analyzing blood-based biomarkers using MFCM.

ANALYST (2022)

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Article Biochemical Research Methods

Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels

Cody Combs et al.

Summary: The study explores the mechanical properties of cells using Deformability cytometry, allowing for rapid characterization of cells and differentiation between cell types. By designing a microfluidic channel and implementing deep learning models, researchers successfully achieved high accuracy classification of sub-populations of HL60 cells.

BIOMICROFLUIDICS (2022)

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Article Chemistry, Analytical

Label-Free Cancer Stem-like Cell Assay Conducted at a Single Cell Level Using Microfluidic Mechanotyping Devices

Miyu Terada et al.

Summary: The mechanical phenotype of cells can serve as a label-free, non-destructive, diagnostic marker, and a microfluidic device is a strong candidate for this translational research. The investigation of HT29 cells revealed a high heterogeneity in cell deformability, which may be a potential mechanical marker of CSCs, contributing to cancer diagnosis and treatment development.

ANALYTICAL CHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

Actin networks regulate the cell membrane permeability during electroporation

Aswin Muralidharan et al.

Summary: In electroporation, disruption of actin networks influences cell membrane permeability, leading to increased uptake of membrane-impermeable molecules with higher temperatures. This suggests a potential lowering of the activation energy barrier for electroporation when actin networks are disrupted, emphasizing the importance of cytoskeletal networks in understanding cell membrane permeability during the delivery of exogenous substances.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

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Article Biochemistry & Molecular Biology

Ca2+roles in electroporation-induced changes of cancer cell physiology: From membrane repair to cell death

Diana Navickaite et al.

Summary: The study investigated the influence of Ca2+ concentration on molecular electrotransfer and release of Calcein from cancer cells, showing that higher calcium ion concentrations reduce the transfer of molecules and release of Calcein. This is related to an enhanced membrane repair mechanism, indicating that the efficiency of molecular electrotransfer can be controlled by regulating Ca2+ concentration.

BIOELECTROCHEMISTRY (2021)

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Article Biophysics

Microscopic impedance cytometry for quantifying single cell shape

Tao Tang et al.

Summary: This study investigated the ability of impedance flow cytometry to measure the shape of single cells/particles. A new metric, tilt index, was proposed to quantify the tilt level of the impedance pulses, which was found to be independent on the trajectories of the micro-objects. Experimental results using a homemade lock-in amplifier on polystyrene beads and Euglena gracilis cells validated the new metric's capability to characterize the shape of single cells/particles without microscopy.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Analytical

Label-Free Multivariate Biophysical Phenotyping-Activated Acoustic Sorting at the Single-Cell Level

Peixian Li et al.

Summary: Biophysical markers of cells have shown promise in studying, characterizing, and classifying different cell types. The lack of cell sorting ability based on multivariate biophysical markers presents a challenge in isolating specific cell types. This study demonstrates label-free and high-throughput acoustic single-cell sorting activated by the characterization of multivariate biophysical phenotypes, providing a solution to the challenges of cell purification without specific molecular biomarkers.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Single-Cell Stretching in Viscoelastic Fluids with Electronically Triggered Imaging for Cellular Mechanical Phenotyping

Minhui Liang et al.

Summary: The study introduces a high-throughput cellular mechanical phenotyping technique based on a microfluidic system, which extracts cell mechanical characteristics through rapid single-cell hydrodynamic stretching and successfully measures the deformability differences of cells.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

A microdevice platform for characterizing the effect of mechanical strain magnitudes on the maturation of iPSC-Cardiomyocytes

Wenkun Dou et al.

Summary: The study developed a microdevice platform that applied cyclical strains of varying magnitudes to iPSC-CMs and measured contractile stress using fluorescent nanobeads. It was found that cyclic strain induced cell alignment and gradually increased contractile force of iPSC-CMs over a 10-day culture period. Contractility positively correlated with sarcomere elongation and increased MYH7 expression in a strain magnitude-dependent manner, indicating mechanical stress optimization for cellular maturation.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

Electrorotation of single microalgae cells during lipid accumulation for assessing cellular dielectric properties and total lipid contents

Yu-Sheng Lin et al.

Summary: This study utilized single-cell electrorotation on Scenedesmus abundans to assess cellular dielectric properties during lipid accumulation and promptly quantify total cellular contents. As the total lipid amount increased from 15.3 wt% to 33.8 wt%, the conductivity and relative permittivity of the inner core decreased by 21.7% and 22.5%, respectively.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Analytical

Label-free mechanoelectrical investigation of single cancer cells by dielectrophoretic-induced stretch assay

Shahriar Shalileh et al.

Summary: The study found that non-cancer cells exhibit a higher electrodeformation response, while malignant cells show a weaker stretch response. Utilizing this difference, a label-free mechanoelectrical chip was proposed for detecting metastatic cancer cells, presenting a new approach for cancer diagnosis and research.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Review Chemistry, Analytical

Microfluidic impedance cytometry for single-cell sensing: Review on electrode configurations

Shu Zhu et al.

Summary: This paper introduces the theoretical background of impedance technique for single-cell analysis and discusses the characteristics of various electrode configurations, including their advantages, disadvantages, and applications. The current limitations and future perspectives of these electrode configurations are also summarized.

TALANTA (2021)

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Article Cell Biology

Functional drug susceptibility testing using single-cell mass predicts treatment outcome in patient-derived cancer neurosphere models

Max A. Stockslager et al.

Summary: This study describes a high-throughput assay that detects subtle changes in the mass of individual drug-treated cancer cells as a potential biomarker for patient treatment response. Validation of this approach in glioblastoma patient-derived neurosphere models showed that changes in cell mass distributions could predict patient overall survival, especially in gliomas with mismatch-repair variants where MGMT is not predictive. This finding suggests that cell mass may be a promising functional biomarker for cancers and drugs lacking genomic biomarkers.

CELL REPORTS (2021)

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Article Biochemical Research Methods

A constriction channel analysis of astrocytoma stiffness and disease progression

P. M. Graybill et al.

Summary: This study utilized a microfluidic constriction channel device to investigate the stiffness of primary brain cancer cells and found that stiffness values were not strongly correlated with cell grade. Using finite element simulations and neural network training, the study provided the first calculation of stiffness for grades II and III astrocytoma cells. Future studies are needed to further elucidate the mechanical properties of astrocytoma across various pathological grades.

BIOMICROFLUIDICS (2021)

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Review Immunology

Necroptosis, pyroptosis and apoptosis: an intricate game of cell death

Damien Bertheloot et al.

Summary: Cell death is a fundamental physiological process in all living organisms, playing important roles in embryonic development, organ maintenance, aging, immune responses, and autoimmunity. Recent research has significantly increased our understanding of the mechanisms orchestrating different types of programmed cell death and how they affect the balance of cell fates. Various modalities of cell death, such as apoptosis, necroptosis, and pyroptosis, have been studied to highlight both common and unique pathways and their impact on the surrounding cells and the organism as a whole.

CELLULAR & MOLECULAR IMMUNOLOGY (2021)

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Review Chemistry, Analytical

Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Zhao Zhang et al.

Summary: Cellular heterogeneity is important in cell-based assays, and electrical impedance sensing technology allows for rapid, non-invasive acquisition of single cell electrical parameters. These parameters are closely related to cellular properties and activities, and devices such as impedance flow cytometry and electrical impedance spectroscopy are used for cell recognition, counting, viability detection, phenotypic assay, and other cell detection. Impedance sensing technology shows promise in single-cell analysis.

BIOSENSORS-BASEL (2021)

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Review Chemistry, Analytical

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

Soojung Kim et al.

Summary: Electrical impedance biosensors combined with microfluidic devices offer high-throughput analysis of biological processes at the single-cell scale, enabling sensitive determination of drug effectiveness and toxicity. The efficiency and performance of the sensors are ultimately determined by the methods of single-cell trapping, while identifying the latest trends opens up opportunities for technological advancement. This leading technology in cell biology, pathology, and pharmacology allows for further understanding of complex cellular functions and mechanisms through precise analysis capabilities.

BIOSENSORS-BASEL (2021)

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Article Chemistry, Multidisciplinary

Direct and Label-Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry

Lingyan Gong et al.

Summary: A novel microfluidic approach for label-free and continuous-flow monitoring of single spheroid/microcarrier based on electrical impedance spectroscopy is reported, with unique impedance signatures identified for optimal spheroid growth and viability monitoring. The platform offers great versatility for multi-parametric analysis of spheroids/microcarriers at high throughput, and can be integrated into bioreactors for long-term and remote monitoring of biomass and cell quality.

SMALL (2021)

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Review Chemistry, Physical

Microfluidic integration for electrochemical biosensor applications

Leona M. Schmidt-Speicher et al.

Summary: Electrochemical biosensors with integrated microfluidics are ideal for miniaturization and integration, suitable for applications in diagnostics, food, and environmental monitoring with real sample measurements. The design of microfluidic devices depends on the type of detection, application, and sample material.

CURRENT OPINION IN ELECTROCHEMISTRY (2021)

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Article Engineering, Biomedical

Circular shaped microelectrodes for single cell electrical measurements for lab-on-a-chip applications

Amina Farooq et al.

Summary: Research focuses on enhancing sensitivity in microfluidic impedimetric sensing of micron and submicron-sized microparticles by investigating novel circular electrodes in a simulation study. Analysis of radial electrode parameters reveals significant impact on signal strength, SNR, and device sensitivity for detecting multiple bioparticles. Optimization of circular electrode geometrical parameters results in >50 dB improvement in SNR, with potential for further adaptation in nanoparticles detection.

BIOMEDICAL MICRODEVICES (2021)

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Article Biochemical Research Methods

Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro

Thomas Collins et al.

Summary: The research team developed a new hybrid spheroid-on-chip in vitro model to study the impact of interstitial fluid flow on cancer spread, utilizing microfluidics technology. They demonstrated the secretion of pro-metastatic factors under flow conditions and highlighted the potential of their device as an alternative to conventional in vivo metastasis assays for studying metastasis biomarkers and therapeutic targets.

BIOMICROFLUIDICS (2021)

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Article Engineering, Biomedical

Portable Automatic Microring Resonator System Using a Subwavelength Grating Metamaterial Waveguide for High-Sensitivity Real-Time Optical-Biosensing Applications

Varun Soni et al.

Summary: This study presented a fully automatic portable biosensing system based on microring resonator, which achieves highly sensitive biomolecular detection through techniques such as microfluidic pump and grating coupler. It can be widely utilized in fields including heavy metal detection, gas sensing, virus examination, and cancer marker diagnosis.

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING (2021)

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Review Materials Science, Biomaterials

In-Line Analysis of Organ-on-Chip Systems with Sensors: Integration, Fabrication, Challenges, and Potential

Stefanie Fuchs et al.

Summary: Organ-on-chip systems are promising new tools for in vitro research in medical, pharmaceutical, and biological fields, offering improved resemblance to in vivo cell culture environments. Integration of in situ analysis methods on-chip allows for better time resolution and continuous measurements, with sensor technology playing a growing role in enhancing these systems.

ACS BIOMATERIALS SCIENCE & ENGINEERING (2021)

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Article Biochemistry & Molecular Biology

Interpretable deep learning uncovers cellular properties in label-free live cell images that are predictive of highly metastatic melanoma

Assaf Zaritsky et al.

Summary: The study utilized a generative neural network and supervised machine learning to classify the metastatic efficiency of melanoma cells, revealing characteristic features of metastatic cells and validating these findings with live cell observations. It demonstrates how artificial intelligence can aid in identifying cell properties predictive of complex phenotypes and integrated cell functions that may be subtle and difficult for human experts to identify in raw imagery.

CELL SYSTEMS (2021)

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Article Chemistry, Multidisciplinary

High-Throughput Characterization of Cell Adhesion Strength Using Long-Channel Constriction-Based Microfluidics

Mingji Wei et al.

Summary: This study introduces a novel method for high-throughput evaluation of cancer cell adhesion strength in long-channel constriction based on friction coefficient. By simplifying the linear elasticity of compressed cells, the influence of cell size heterogeneity is effectively mitigated in determining adhesion strength.

ACS SENSORS (2021)

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Review Nanoscience & Nanotechnology

Novel microfluidic approaches to circulating tumor cell separation and sorting of blood cells: A review

A. Farahinia et al.

Summary: The separation of circulating tumor cells (CTCs) is crucial for cancer diagnostics, progression analyses, and treatment efficiency. Researchers are exploring various techniques for quick and continuous CTC separation, with inertial microfluidic devices showing promise for their simple structure and setup. However, challenges like the need for pumps, limited cell movement control, and channel clogging still exist.

JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES (2021)

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Article Biochemical Research Methods

Submicron-precision particle characterization in microfluidic impedance cytometry with double differential electrodes

Jianwei Zhong et al.

Summary: A label-free high-throughput impedance-based microfluidic flow cytometry system with a novel double differential electrode design has been proposed for submicron particle detection, achieving high signal-to-noise ratio and accuracy in sizing microparticles. Experimental results show a strong correlation between the measured particle sizes and manufacturers' datasheets, as well as consistent population ratios measurements with commercial flow cytometry results.

LAB ON A CHIP (2021)

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Article Biochemical Research Methods

A microfluidic device enabling deterministic single cell trapping and release

Huichao Chai et al.

Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.

LAB ON A CHIP (2021)

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Article Physics, Fluids & Plasmas

Single-cell mechanical analysis and tension quantification via electrodeformation relaxation

Seyedsajad Moazzeni et al.

Summary: This study analyzed the mechanical behavior and cortical tension of single cells using electrodeformation relaxation, finding differences in mechanical response characteristics of the cortex in different pulse duration ranges.

PHYSICAL REVIEW E (2021)

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Article Chemistry, Analytical

Multi-frequency single cell electrical impedance measurement for label-free cell viability analysis

Jianwei Zhong et al.

Summary: This study presents a label-free impedance-based approach for cell viability assessment by characterizing multiple electrical cellular phenotypes in a high-throughput manner, introducing a concept called the complex opacity spectrum for improved discrimination of live and dead cells. The approach successfully discriminated between living and dead cells in different experimental scenarios and provides highly accurate and consistent cell viability analysis, validated by commercial fluorescence-based flow cytometry.

ANALYST (2021)

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Article Chemistry, Physical

Optical quantification of intracellular mass density and cell mechanics in 3D mechanical confinement

Sadra Bakhshandeh et al.

Summary: Recent studies have shown that the nucleus of cells in 3D display a higher dry mass density than the cytoplasm, unlike in 2D cultures. Using non-invasive techniques, such as optical diffraction tomography and Brillouin microscopy, researchers were able to quantify intracellular biophysical properties and found that increasing the stiffness of the hydrogel resulted in higher modulus for both the nuclei and cytoplasm of cells in stiff 3D alginate. This improved understanding of biological processes such as dormancy, apoptosis, cell growth, and stem cell differentiation.

SOFT MATTER (2021)

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Review Biochemical Research Methods

Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics

Carlos Honrado et al.

Summary: Microfluidic impedance cytometry is a label-free and high-throughput method for stratifying cellular systems based on electrophysiology, with applications ranging from life science research to precision medicine. Novel chip designs and data analytic strategies are laying the foundation for multiparametric cell characterization and subpopulation distinction, essential for understanding biological function and disease progression. Emerging developments in the technique, such as device design, data analytics, and phenotyping applications, offer exciting prospects for future challenges and directions in label-free quantification and isolation of subpopulations in heterogeneous biosystems.

LAB ON A CHIP (2021)

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Article Biochemical Research Methods

A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry

Carlos Honrado et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

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Article Automation & Control Systems

Automated Parallel Electrical Characterization of Cells Using Optically-Induced Dielectrophoresis

Na Liu et al.

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING (2020)

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Review Oncology

Strategies for enrichment of circulating tumor cells

Xinchun Li et al.

TRANSLATIONAL CANCER RESEARCH (2020)

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Article Multidisciplinary Sciences

Construction of a human cell landscape at single-cell level

Xiaoping Han et al.

NATURE (2020)

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Article Biochemical Research Methods

A comparison of microfluidic methods for high-throughput cell deformability measurements

Marta Urbanska et al.

NATURE METHODS (2020)

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Article Chemistry, Analytical

Label-Free and Simultaneous Mechanical and Electrical Characterization of Single Plant Cells Using Microfluidic Impedance Flow Cytometry

Ziyu Han et al.

ANALYTICAL CHEMISTRY (2020)

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Review Biotechnology & Applied Microbiology

Mechanical properties of single cells: Measurement methods and applications

Yansheng Hao et al.

BIOTECHNOLOGY ADVANCES (2020)

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Article Chemistry, Multidisciplinary

Nanocytometer for smart analysis of peripheral blood and acute myeloid leukemia: a pilot study

Julian Schuett et al.

NANO LETTERS (2020)

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Review Biotechnology & Applied Microbiology

Micro/Nano fabricated cantilever based biosensor platform: A review and recent progress

Aviru Kumar Basu et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

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Review Chemistry, Applied

Dielectrophoretic Manipulation of Cancer Cells and Their Electrical Characterization

Ina Turcan et al.

ACS COMBINATORIAL SCIENCE (2020)

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Article Multidisciplinary Sciences

Simultaneous time-varying viscosity, elasticity, and mass measurements of single adherent cancer cells across cell cycle

Olaoluwa O. Adeniba et al.

SCIENTIFIC REPORTS (2020)

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Article Engineering, Biomedical

Analysis on reversible/irreversible electroporation region in lung adenocarcinoma cell model in vitro with electric pulses delivered by needle electrodes

Yanpeng Lv et al.

PHYSICS IN MEDICINE AND BIOLOGY (2020)

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Review Chemistry, Analytical

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Lexi L. Crowell et al.

MICROMACHINES (2020)

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Proceedings Paper Biophysics

Broadband Measurement Setup for Cell Electrorotation

Pawel Barmutat et al.

2020 IEEE MTT-S INTERNATIONAL MICROWAVE BIOMEDICAL CONFERENCE (IMBIOC) (2020)

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Review Pathology

Precancerous lesions of the stomach, gastric cancer and hereditary gastric cancer syndromes

Irene Gullo et al.

PATHOLOGICA (2020)

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Article Nanoscience & Nanotechnology

Matrix Stiffness Regulates Chemosensitivity, Stemness Characteristics, and Autophagy in Breast Cancer Cells

Yan Li et al.

ACS APPLIED BIO MATERIALS (2020)

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Article Biochemical Research Methods

Mechanical phenotyping of breast cell lines by in-flow deformation-dependent dynamics under tuneable compressive forces

David Dannhauser et al.

LAB ON A CHIP (2020)

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Review Biochemical Research Methods

Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: origin, challenges and opportunities

Hugo Daguerre et al.

LAB ON A CHIP (2020)

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Article Biophysics

Overcoming the sensitivity vs. throughput tradeoff in Coulter counters: A novel side counter design

Daniel T. Bacheschi et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Materials Science, Biomaterials

A Machine Learning-Assisted Nanoparticle-Printed Biochip for Real-Time Single Cancer Cell Analysis

Kushal Joshi et al.

ADVANCED BIOSYSTEMS (2020)

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Article Chemistry, Analytical

Label-free counting of affinity-enriched circulating tumor cells (CTCs) using a thermoplastic micro-Coulter counter (μCC)

Cong Kong et al.

ANALYST (2020)

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Article Chemistry, Analytical

Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation

Shikiho Kawai et al.

ANALYST (2020)

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Article Biochemical Research Methods

A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging

Pablo Gimenez-Gomez et al.

LAB ON A CHIP (2020)

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Review Engineering, Multidisciplinary

Cells Electrical Characterization: Dielectric Properties, Mixture, and Modeling Theories

Nida Nasir et al.

JOURNAL OF ENGINEERING (2020)

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Article Engineering, Biomedical

Differentiation Between Normal and Cancerous Human Urothelial Cell Lines Using Micro-Electrical Impedance Spectroscopy at Multiple Frequencies

Hyeon Woo Kim et al.

JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING (2019)

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Article Biophysics

Cells Under Stress: An Inertial-Shear Microfluidic Determination of Cell Behavior

Fern J. Armistead et al.

BIOPHYSICAL JOURNAL (2019)

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Article Biophysics

Biophysical properties of cells for cancer diagnosis

Sharda Yadav et al.

JOURNAL OF BIOMECHANICS (2019)

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Review Chemistry, Analytical

Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications

Tao Luo et al.

MICROMACHINES (2019)

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Article Chemistry, Analytical

A Microfluidic Micropipette Aspiration Device to Study Single-Cell Mechanics Inspired by the Principle of Wheatstone Bridge

Yong-Jiang Li et al.

MICROMACHINES (2019)

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Article Biophysics

Biophysical phenotyping of single cells using a differential multiconstriction microfluidic device with self-aligned 3D electrodes

Dahou Yang et al.

BIOSENSORS & BIOELECTRONICS (2019)

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Article Biochemical Research Methods

On-chip technology for single-cell arraying, electrorotation-based analysis and selective release

Kevin Keim et al.

ELECTROPHORESIS (2019)

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Article Chemistry, Analytical

Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis

Wenchang Zhang et al.

SENSORS AND ACTUATORS B-CHEMICAL (2019)

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Article Biochemical Research Methods

Biophysical phenotyping of cells via impedance spectroscopy in parallel cyclic deformability channels

Xiang Ren et al.

BIOMICROFLUIDICS (2019)

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Article Biochemical Research Methods

Toward Microfluidic Label-Free Isolation and Enumeration of Circulating Tumor Cells from Blood Samples

Camille Raillon et al.

CYTOMETRY PART A (2019)

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Article Multidisciplinary Sciences

High-Throughput, Time-Resolved Mechanical Phenotyping of Prostate Cancer Cells

Yuri Belotti et al.

SCIENTIFIC REPORTS (2019)

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Article Nanoscience & Nanotechnology

Toward point-of-care assessment of patient response: a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning

Karan Ahuja et al.

MICROSYSTEMS & NANOENGINEERING (2019)

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Article Engineering, Electrical & Electronic

A novel impedance sensing approach for precise electromechanical characterization of cells

Karthik Mahesh et al.

JOURNAL OF MICROMECHANICS AND MICROENGINEERING (2019)

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Article Chemistry, Analytical

Particle Self-Aligning, Focusing, and Electric Impedance Microcytometer Device for Label-Free Single Cell Morphology Discrimination and Yeast Budding Analysis

Xinwu Xie et al.

ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

A Microfluidic Device Integrating Impedance Flow Cytometry and Electric Impedance Spectroscopy for High-Efficiency Single-Cell Electrical Property Measurement

Yongxiang Feng et al.

ANALYTICAL CHEMISTRY (2019)

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Article Biophysics

Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies

Blanca Gonzalez-Bermudez et al.

BIOPHYSICAL JOURNAL (2019)

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Review Oncology

The Extracellular, Cellular, and Nuclear Stiffness, a Trinity in the Cancer Resistome-A Review

Sara Sofia Deville et al.

FRONTIERS IN ONCOLOGY (2019)

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Article Biochemical Research Methods

Microfluidic impedance cytometry device with N-shaped electrodes for lateral position measurement of single cells/particles

Dahou Yang et al.

LAB ON A CHIP (2019)

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Article Biochemical Research Methods

Processing code-multiplexed Coulter signals via deep convolutional neural networks

Ningquan Wang et al.

LAB ON A CHIP (2019)

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Article Chemistry, Analytical

Measuring the apparent viscosities of single cells by tracking the entire deformation dynamics in microfluidic channels

Wei-Mo Yuan et al.

ANALYTICAL METHODS (2019)

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Article Chemistry, Analytical

Electrical Impedance Measurements of Biological Cells in Response to External Stimuli

Amin Mansoorifar et al.

ANALYTICAL CHEMISTRY (2018)

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Article Chemistry, Analytical

Electrodeformation-Based Biomechanical Chip for Quantifying Global Viscoelasticity of Cancer Cells Regulated by Cell Cycle

Yao Teng et al.

ANALYTICAL CHEMISTRY (2018)

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Editorial Material Biochemistry & Molecular Biology

Seeing More: A Future of Augmented Microscopy

Devin P. Sullivan et al.

CELL (2018)

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Article Biochemical Research Methods

3D cell electrorotation and imaging for measuring multiple cellular biophysical properties

Liang Huang et al.

LAB ON A CHIP (2018)

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Review Biotechnology & Applied Microbiology

Lab-on-a-Chip Platforms for Biophysical Studies of Cancer with Single-Cell Resolution

Vasudha C. Shukla et al.

TRENDS IN BIOTECHNOLOGY (2018)

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Article Cell Biology

Microfluidic cell sorting by stiffness to examine heterogenic responses of cancer cells to chemotherapy

Muhymin Islam et al.

CELL DEATH & DISEASE (2018)

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Article Nanoscience & Nanotechnology

Characterizing cellular mechanical phenotypes with mechano-node-pore sensing

Junghyun Kim et al.

MICROSYSTEMS & NANOENGINEERING (2018)

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Article Multidisciplinary Sciences

Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel

Binita Nath et al.

SCIENTIFIC REPORTS (2018)

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Article Chemistry, Analytical

Characterizing Deformability and Electrical Impedance of Cancer Cells in a Microfluidic Device

Ying Zhou et al.

ANALYTICAL CHEMISTRY (2018)

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Article Biophysics

Actin cytoskeleton stiffness grades metastatic potential of ovarian carcinoma Hey A8 cells via nanoindentation mapping

Z. L. Zhou et al.

JOURNAL OF BIOMECHANICS (2017)

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Article Biochemical Research Methods

A combined experimental and theoretical approach towards mechanophenotyping of biological cells using a constricted microchannel

A. Raj et al.

LAB ON A CHIP (2017)

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Review Cell Biology

Mechanobiology of YAP and TAZ in physiology and disease

Tito Panciera et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2017)

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Article Biophysics

Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties

Kendra D. Nyberg et al.

BIOPHYSICAL JOURNAL (2017)

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Article Biochemistry & Molecular Biology

Tumor matrix stiffness promotes metastatic cancer cell interaction with the endothelium

Steven E. Reid et al.

EMBO JOURNAL (2017)

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Article Biophysics

Unbiased High-Precision Cell Mechanical Measurements with Microconstrictions

Janina R. Lange et al.

BIOPHYSICAL JOURNAL (2017)

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Article Biophysics

Measuring Cell Viscoelastic Properties Using a Microfluidic Extensional Flow Device

Lionel Guillou et al.

BIOPHYSICAL JOURNAL (2016)

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Article Biochemical Research Methods

The physical origins of transit time measurements for rapid, single cell mechanotyping

Kendra D. Nyberg et al.

LAB ON A CHIP (2016)

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Article Biotechnology & Applied Microbiology

Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate

Mark M. Stevens et al.

NATURE BIOTECHNOLOGY (2016)

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Review Chemistry, Analytical

A Comprehensive Review of Optical Stretcher for Cell Mechanical Characterization at Single-Cell Level

Tie Yang et al.

MICROMACHINES (2016)

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Article Materials Science, Biomaterials

Discrimination Between Normal and Cancerous Cells Using AFM

Malgorzata Lekka

BIONANOSCIENCE (2016)

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Article Biochemical Research Methods

Differential microfluidic sensor on printed circuit board for biological cells analysis

Dongyuan Shi et al.

ELECTROPHORESIS (2015)

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Review Biochemistry & Molecular Biology

Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization

Jian Chen et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2015)

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Article Cell Biology

Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis

Sungmin Son et al.

JOURNAL OF CELL BIOLOGY (2015)

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Article Biochemical Research Methods

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Oliver Otto et al.

NATURE METHODS (2015)

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Article Chemistry, Analytical

Classification of Cells with Membrane Staining and/or Fixation Based on Cellular Specific Membrane Capacitance and Cytoplasm Conductivity

Song-Bin Huang et al.

MICROMACHINES (2015)

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Article Biochemical Research Methods

Microfluidic impedance cytometry of tumour cells in blood

Daniel Spencer et al.

BIOMICROFLUIDICS (2014)

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Article Biophysics

An Engineered Membrane to Measure Electroporation: Effect of Tethers and Bioelectronic Interface

William Hoiles et al.

BIOPHYSICAL JOURNAL (2014)

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Article Engineering, Electrical & Electronic

Characterization of Dielectric Responses of Human Cancer Cells in the Terahertz Region

Keiichiro Shiraga et al.

JOURNAL OF INFRARED MILLIMETER AND TERAHERTZ WAVES (2014)

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Article Physics, Applied

A nonlinear electromechanical coupling model for electropore expansion in cell electroporation

Peigang Deng et al.

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2014)

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Article Biochemical Research Methods

Red blood cell fatigue evaluation based on the close-encountering point between extensibility and recoverability

Shinya Sakuma et al.

LAB ON A CHIP (2014)

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Article Chemistry, Analytical

Monitoring cellular activities of cancer cells using impedance sensing devices

Rangadhar Pradhan et al.

SENSORS AND ACTUATORS B-CHEMICAL (2014)

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Review Chemistry, Analytical

Recent advances in the development of single cell analysis-A review

Karel Kleparnik et al.

ANALYTICA CHIMICA ACTA (2013)

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Article Chemistry, Analytical

Microfluidic Chip with Integrated Electrical Cell-Impedance Sensing for Monitoring Single Cancer Cell Migration in Three-Dimensional Matrixes

Tien Anh Nguyen et al.

ANALYTICAL CHEMISTRY (2013)

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Review Cell Biology

Mechanobiology and Developmental Control

Tadanori Mammoto et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 29 (2013)

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Article Biochemical Research Methods

A label-free DC impedance-based microcytometer for circulating rare cancer cell counting

Hyoungseon Choi et al.

LAB ON A CHIP (2013)

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Article Multidisciplinary Sciences

Characterizing deformability and surface friction of cancer cells

Sangwon Byun et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

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Article Chemistry, Analytical

Microfluidics-Based Assessment of Cell Deformability

Andrea Adamo et al.

ANALYTICAL CHEMISTRY (2012)

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Article Cell Biology

Cell adhesion in embryo morphogenesis

Vanessa Barone et al.

CURRENT OPINION IN CELL BIOLOGY (2012)

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Article Biochemical Research Methods

Direct observation of mammalian cell growth and size regulation

Sungmin Son et al.

NATURE METHODS (2012)

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Review Materials Science, Multidisciplinary

Linear and Nonlinear Rheology of Living Cells

Philip Kollmannsberger et al.

ANNUAL REVIEW OF MATERIALS RESEARCH, VOL 41 (2011)

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Article Biochemical Research Methods

Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

Ning Bao et al.

LAB ON A CHIP (2011)

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Article Biochemical Research Methods

On-chip measurements of cell compressibility via acoustic radiation

Deny Hartono et al.

LAB ON A CHIP (2011)

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Article Biochemistry & Molecular Biology

The influence of different membrane components on the electrical stability of bilayer lipid membranes

Iris van Uitert et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2010)

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Review Biochemistry & Molecular Biology

The role of physiological heterogeneity in microbial population behavior

Mary E. Lidstrom et al.

NATURE CHEMICAL BIOLOGY (2010)

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Article Chemistry, Analytical

Microfluidic electroporative flow cytometry for studying single-cell biomechanics

Ning Bao et al.

ANALYTICAL CHEMISTRY (2008)

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Article Engineering, Biomedical

Microfluidic device for cell capture and impedance measurement

Ling-Sheng Jang et al.

BIOMEDICAL MICRODEVICES (2007)

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Article Multidisciplinary Sciences

Weighing of biomolecules, single cells and single nanoparticles in fluid

Thomas P. Burg et al.

NATURE (2007)

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Article Biotechnology & Applied Microbiology

Atomic force microscopy in biology: technology and techniques

N. Gadegaard

BIOTECHNIC & HISTOCHEMISTRY (2006)

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Article Biochemical Research Methods

Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing

S Gawad et al.

LAB ON A CHIP (2001)

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