The simultaneous voltammetric detection of guanine and inosine using a hybrid nanocomposite of multi-walled carbon nanotubes and iron oxide nanoparticles

In this proof-of-concept study, a graphite paste electrode was modified with a novel hybrid nanocomposite of multi-walled carbon nanotubes and iron-oxide nanoparticles for the simultaneous voltammetric detection of guanine and inosine. The nanocomposite was characterized using transmission electron microscopy. The incorporation of iron oxide nanoparticles with multiwalled carbon nanotubes to the modified graphite paste electrode provided an enhanced electrocatalytic activity as studied by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry. The linearity ranges were 0.1-9.0 mu M and 0.5-55 mu M with a detection limit of 0.05 mu M and 0.12 mu M for guanine and inosine, respectively. Moreover, chronoamperometric studies were carried out for the determination of diffusion coefficients, and heterogenous rate constants of guanine and inosine. The sensor was also used for the simultaneous detection of guanine and inosine in commercially available bovine serum samples with recovery values ranging from 96 % to 104 %. We envisage the nanocomposite-modified sensor provides a promising platform for the detection of guanine and inosine in pharmaceutical formulations and biological samples as biomarkers of oxidative damage related to cancer research. image

Automated summary

In this proof-of-concept study, a graphite paste electrode modified with a hybrid nanocomposite of multi-walled carbon nanotubes and iron-oxide nanoparticles was developed for the simultaneous voltammetric detection of guanine and inosine. The modified electrode showed enhanced electrocatalytic activity and good detection performance in commercially available bovine serum samples. This nanocomposite-modified sensor holds promise for the detection of guanine and inosine in pharmaceutical formulations and biological samples, serving as biomarkers of oxidative damage related to cancer research.