期刊
CHEMICAL COMMUNICATIONS
卷 51, 期 78, 页码 14671-14673出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc05921f
关键词
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资金
- National Natural Science Foundation of China [21375079]
- Natural Science Foundation of Shandong province, China [ZR2014BQ029]
- China Postdoctoral Science Foundation [2015T80732, 2014M550369]
- Project of Development of Science and Technology of Shandong Province, China [2013GZX20109]
A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.
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