期刊
DYES AND PIGMENTS
卷 215, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2023.111246
关键词
Fluorescent probes; Biothiol; Cell imaging; Aggregation-induced emission; Donor-Acceptor
The synthesis of quinoline-malononitrile (QM)-based fluorescent probes with and without iodine substitution surrounding its phenolic ring was studied. It was found that iodine substitution compromised the aggregation-induced emission of QM but enhanced the sensitivity for biothiols including L-cystine (L-Cys) detection in weakly acidic to neutral aqueous solutions by reducing the pKa of the QM fluorogen. The probe also enabled the visualization of fluctuating L-Cys levels in cells.
The development of long-wavelength fluorescent probes with a pH working range from weakly acidic to neutral is important for cell-imaging studies. In this study, quinoline-malononitrile (QM)-based fluorescent probes without and with iodine substitution surrounding its phenolic ring are synthesized. The iodine substitution manner was found to compromise the aggregation-induced emission of QM whilst resulting in a significantly enhanced sensitivity for biothiols including L-cystine (L-Cys) detection in weakly acidic to neutral aqueous buffer solutions through reducing the pKa of the QM fluorogen. The probe also effectively achieved the fluorescence visualization of the fluctuation in L-Cys levels in cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据