期刊
DYES AND PIGMENTS
卷 215, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2023.111295
关键词
ATP; Fluorescent probe; Tetraphenylethylene; Imaging; Mitochondria
A tetraphenylethylene fluorophore based on D-pi-A structure was used to design and synthesize the TPE-PyFP probe for selective detection of ATP. TPE-Py-FP showed a turn-on response after adding ATP and had a wide linear range and low limit of detection. The detection mechanism was mainly the electrostatic interaction between the positively charged sites of TPE-Py-FP and the negatively charged triphosphate structure of ATP.
A tetraphenylethylene fluorophore based on D-pi-A structure was employed to design and synthesize the TPE-PyFP probe for selective detection of ATP. TPE-Py-FP had the advantages of large Stokes shift, great hydrophilicity and good selectivity. Especially, the pyridine portion and quaternary ammonium structure of TPE-Py-FP had positively charged sites, which could be used as recognition groups to interact with the negatively charged triphosphate structure of ATP. Then the fluorescence emission of TPE-Py-FP at 580 nm showed a turn-on response after adding ATP. Moreover, this probe could rapidly respond to ATP over a wide linear range of 10-120 mu M and the limit of detection (LOD) was 2.76 mu M. The zeta potential and dynamic light scattering experiments revealed that the detection mechanism was mainly the electrostatic adsorption interaction between the dual positively charged sites of TPE-Py-FP and the negatively charged triphosphate structure of ATP. Additionally, TPE-Py-FP could localize in mitochondria specifically with a Pearson's coefficient of 0.81, and show great utility in monitoring the fluctuation of mitochondrial ATP level.
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