Development and validation of a real-time PCR test to detect Bartonella quintana in clinical samples

Article Pathology

Evaluation of ssrA-targeted real time PCR for the detection of Bartonella species in human clinical samples and reflex sequencing for species-level identification

Anna Vesty et al.

Summary: This study compared the performance of a real-time PCR assay targeting the ssrA gene to conventional PCR and sequencing methods for detecting and differentiating Bartonella species in human clinical samples. The real-time ssrA PCR performed better for non-Bartonella henselae species and improved the sensitivity of B. henselae detection.

PATHOLOGY (2022)

添加到收藏夹

Article Microbiology

Bartonella Endocarditis in Spain: Case Reports of 21 Cases

Lara Garcia-Alvarez et al.

Summary: This study describes the epidemiology, clinical characteristics, management, and outcomes of Bartonella infective endocarditis (IE). Bartonella IE is rare in the GAMES cohort, with Bartonella henselae being the most common causative agent. Cardiac failure is the main presentation, often affecting the aortic valve. Diagnosis relies on serology and PCR, and treatment involves antimicrobial therapy and surgery. The prognosis for patients is generally good.

PATHOGENS (2022)

添加到收藏夹

Article Microbiology

Simultaneous Detection and Differentiation of Clinically Relevant Relapsing Fever Borrelia with Semimultiplex Real-Time PCR

Elizabeth A. Dietrich et al.

Summary: The development of a real-time semimultiplex PCR assay has improved the diagnosis and classification of relapsing fever (RF) by detecting multiple RF borreliae and aiding in the clinical management of RF patients. This single-assay approach accurately detects and classifies RF borreliae, with high specificity and sensitivity.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

添加到收藏夹

Article Immunology

Bartonella Seroreactivity Among Persons Experiencing Homelessness During an Outbreak of Bartonella quintana in Denver, Colorado, 2020

David W. McCormick et al.

Summary: In a recent outbreak of Bartonella quintana disease in Denver, 15% of 241 homeless individuals tested for SARS-CoV-2 were found to be seroreactive for Bartonella. Improved recognition of B. quintana disease and prevention of louse infestation are crucial for this vulnerable population.

OPEN FORUM INFECTIOUS DISEASES (2021)

添加到收藏夹

Article Microbiology