DLX gene expression in the developing chick pharyngeal arches and relationship to endothelin signaling and avian jaw patterning

Background: A hinged jaw that articulates with the skull base is a striking feature of the vertebrate head and has been greatly modified between, and within, vertebrate classes. Genes belonging to the DLX homeobox family are conserved mediators of local signaling pathways that distinguish the dorsal and ventral aspects of the first pharyngeal arch. Specifically, a subset of DLX genes are expressed in the cranial neural crest-derived mandibular ectomesenchyme in response to ventral endothelin signaling, an important step that confers the first arch with maxillary and mandibular identities. Downstream targets of DLX genes then execute the morphogenetic processes that lead to functional jaws. Identifying lineage-specific variations in DLX gene expression and the regulatory networks downstream of DLX action is necessary to understand how different kinds of jaws evolved. Results: Here, we describe and compare the expression of all six DLX genes in the chick pharyngeal arches, focusing on the period of active patterning in the first arch. Disruption of endothelin signaling results in the down-regulation of ventral-specific DLX genes and confirms their functional role in avian jaw patterning. Conclusions: This expression resource will be important for comparative embryology and for identifying synexpression groups of DLX-regulated genes in the chick.

Automated summary

This study describes and compares the expression of all six DLX genes in the chick pharyngeal arches, specifically focusing on the period of active patterning in the first arch. Disrupting endothelin signaling leads to the down-regulation of ventral-specific DLX genes, confirming their functional role in avian jaw patterning. This research is important for comparative embryology and identifying synexpression groups of DLX-regulated genes in the chick.