4.7 Article

Mouse Pramel1 regulates spermatogonial development by inhibiting retinoic acid signaling during spermatogenesis

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DEVELOPMENT
卷 150, 期 21, 页码 -

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COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201907

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PRAMEL1; Retinoic acid; Sertoli cell-only; Spermatogenesis; Transgenic mice

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This study reveals the involvement of PRAMEL1 in the initiation and maintenance of spermatogenesis by regulating the responsiveness of prospermatogonia to retinoic acid (RA) and their homing process during spermatogenesis initiation. Pramel1 deficiency leads to altered fecundity in males and the occurrence of a Sertoli cell-only phenotype.
Spermatogenesis begins when cell fate-committed prospermatogonia migrate to the basement membrane and initiate spermatogenesis in response to retinoic acid (RA) in the neonatal testis. The underlying cellular and molecular mechanisms in this process are not fully understood. Here, we report findings on the involvement of a cancer/ testis antigen, PRAMEL1, in the initiation and maintenance of spermatogenesis. By analyzing mouse models with either global or conditional Pramel1 inactivation, we found that PRAMEL1 regulates the RA responsiveness of the subtypes of prospermatogonia in the neonatal testis, and affects their homing process during the initiation of spermatogenesis. Pramel1 deficiency led to increased fecundity in juvenile males and decreased fecundity in mature males. In addition, Pramel1 deficiency resulted in a regional Sertoli cell-only phenotype during the first round of spermatogenesis, which was rescued by administration of the RA inhibitor WIN18,446, suggesting that PRAMEL1 functions as an inhibitor of RA signaling in germ cells. Overall, our findings suggest that PRAMEL1 fine-tunes RA signaling, playing a crucial role in the proper establishment of the first and subsequent rounds of spermatogenesis.

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