期刊
DEVELOPMENT
卷 150, 期 17, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201717
关键词
KEY WORDS; Chondroitin sulfate; Ovary; Basement membrane; Muscle sheath; Chsy; Drosophila
Heparan sulfate (HS) and chondroitin sulfate (CS) are glycosaminoglycans that are found in most animal species. This study focused on the role of CS in development by generating mutants for Chondroitin sulfate synthase (Chsy) in Drosophila. The mutants showed altered stiffness of the basement membrane and muscle dysfunction in the ovary, leading to degradation of the organ structure.
Heparan sulfate (HS) and chondroitin sulfate (CS) are evolutionarily conserved glycosaminoglycans that are found in most animal species, including the genetically tractable model organism Drosophila. In contrast to extensive in vivo studies elucidating co-receptor functions of Drosophila HS proteoglycans (PGs), only a limited number of studies have been conducted for those of CSPGs. To investigate the global function of CS in development, we generated mutants for Chondroitin sulfate synthase (Chsy), which encodes the Drosophila homolog of mammalian chondroitin synthase 1, a crucial CS biosynthetic enzyme. Our characterizations of the Chsy mutants indicated that a fraction survive to adult stage, which allowed us to analyze the morphology of the adult organs. In the ovary, Chsy mutants exhibited altered stiffness of the basement membrane and muscle dysfunction, leading to a gradual degradation of the gross organ structure as mutant animals aged. Our observations show that normal CS function is required for the maintenance of the structural integrity of the ECM and gross organ architecture.
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