期刊
DEVELOPMENT
卷 150, 期 16, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201141
关键词
Mechanotransduction; Tendon; Tenocyte differentiation; Enthesis; Zebrafish
The force from tendons and ligaments is transmitted to the skeleton through entheseal cells, and the regional organization and expression of transcription factors play a vital role in force transmission. Muscle contraction force helps establish the specialized organization of extracellular matrix and regulate the expression level of transcription factors in entheseal cells.
Entheses transmit force from tendons and ligaments to the skeleton. Regional organization of enthesis extracellular matrix (ECM) generates differences in stiffness required for force transmission. Two key transcription factors co-expressed in entheseal tenocytes, scleraxis (Scx) and Sox9, directly control production of enthesis ECM components. Formation of embryonic craniofacial entheses in zebrafish coincides with onset of jaw movements, possibly in response to the force of muscle contraction. We show dynamic changes in scxa and sox9a mRNA levels in subsets of entheseal tenocytes that correlate with their roles in force transmission. We also show that transcription of a direct target of Scxa, Col1a, in enthesis ECM is regulated by the ratio of scxa to sox9a expression. Eliminating muscle contraction by paralyzing embryos during early stages of musculoskeletal differentiation alters relative levels of scxa and sox9a in entheses, primarily owing to increased sox9a expression. Force dependent TGF-beta (TGF beta) signaling is required to maintain this balance of scxa and sox9a expression. Thus, force from muscle contraction helps establish a balance of transcription factor expression that controls specialized ECM organization at the tendon enthesis and its ability to transmit force.
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