☆ 4.7 Review

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2023)

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Review Agriculture, Multidisciplinary

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Lijuan Yin et al.

Summary: Foodborne viruses are recognized as significant threats to food safety and human health. Rapid and accurate detection is crucial for food safety control. This review focuses on the progress of detection methods for foodborne viruses and summarizes current methods including electron microscopy, immunoassay, molecular technology, biosensors, and CRISPR/Cas-based detection technology.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2023)

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Article Chemistry, Multidisciplinary

Cascade DNA Circuits Mediated CRISPR-Cas12a Fluorescent Aptasensor based on Multifunctional Fe3O4@hollow-TiO2@MoS2 Nanochains for Tetracycline Determination

Yan Lv et al.

Summary: For the first time, the combination of CRISPR-Cas12a system, aptamer, cascaded dynamic DNA network circuits, and Fe3O4@hollow-TiO2@MoS2 nanochains (Fe3O4@h-TiO2@MoS2 NCs) is used to construct an efficient sensing platform for tetracycline (TC) analysis. This study demonstrates the specific recognition, transduction, and amplification of the target through the aptamer recognition module and dual amplification dynamic DNA network, leading to the recovery of the fluorescence signal. The synthesized multifunctional Fe3O4@h-TiO2@MoS2 NCs composites also exhibit magnetic separability and photocatalytic degradation ability.

SMALL (2023)

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Editorial Material Biotechnology & Applied Microbiology

Comparison of CRISPR/Cas and Argonaute for nucleic acid tests

Yaru Li et al.

Summary: This study aims to compare the similarities, differences, and complementarities between the CRISPR/Cas system and Argonaute (Ago) as nucleases, in order to guide the design of novel nucleic acid tests.

TRENDS IN BIOTECHNOLOGY (2023)

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Article Chemistry, Multidisciplinary

Sensitive Small Molecule Aptasensing based on Hybridization Chain Reaction and CRISPR/Cas12a Using a Portable 3D-Printed Visualizer

Long Ma et al.

Summary: The study reports a versatile biosensing platform called SMART-Cas12a for non-nucleic acid small-molecule detection. The platform utilizes functional nucleic acid (aptamer) to trigger hybridization chain reaction cascade signal amplification, followed by integration of the CRISPR/Cas system to recognize and activate the trans-cleavage of the amplified products. This converts the target to nucleic acid signals, which can be visualized and analyzed using a customized 3D-printed visualizer and a home-made App-enabled smartphone.

ACS SENSORS (2023)

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Article Chemistry, Analytical

An electrochemical biosensor for the highly sensitive detection of Staphylococcus aureus based on SRCA-CRISPR/Cas12a

Luqi Huang et al.

Summary: A novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) and CRISPR/Cas12a system was developed for accurate detection of Staphylococcus aureus. The biosensor showed high sensitivity, specificity, and accuracy, providing a potential powerful platform for the detection of S. aureus.

TALANTA (2023)

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Article Engineering, Environmental

A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

Long Ma et al.

Summary: We developed a versatile biosensing platform, SCENT-Cas (Silver nanoCluster Empowered Nucleic acids Test using CRISPR/Cas12a), for ultrasensitive detection of pathogenic bacteria. This platform used the isothermal amplification of the invA gene of Salmonella typhimurium to trigger trans-cleavage of CRISPR/Cas12a. The trans-cleavage was then utilized to degrade single-stranded DNA and create a ratiometric fluorescence biosensing platform. This method enabled the detection of 1 CFU/mL S. typhi with a dynamic range from 1 to 108 CFU/mL, and showed promise for real food sample testing.

JOURNAL OF HAZARDOUS MATERIALS (2023)

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Article Engineering, Environmental

A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel

Long Ma et al.

Summary: This study proposes a nanobioassay called OVER-SARS-CoV-2, which combines SERS-read and CRISPR/Cas technology to enable fast, highly sensitive, accurate, and portable detection of SARS-CoV-2 in a single vessel without amplification and interference. Gold nanoparticles are combined with Raman reporting molecules and single-stranded DNA probes to convert nucleic acid signals into SERS signals. By customizing a vessel, the steps of reverse transcription, Cas12a trans-cleavage, and SERS nanoprobes crosslinking are integrated. The nanobioassay is able to detect SARS-CoV-2 as low as 200 copies/mL within 45 minutes without pre-amplification, and has been validated with clinical samples and complex environmental and food samples.

JOURNAL OF HAZARDOUS MATERIALS (2023)

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Article Engineering, Environmental

The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets

Yu Wang et al.

Summary: A CRISPR-Cas12a-based fluorescence/colorimetric biosensor was developed for efficient detection of small molecules and protein targets, with significant implications in the fields of food safety, environmental monitoring, and clinical diagnosis.

JOURNAL OF HAZARDOUS MATERIALS (2023)

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Review Biotechnology & Applied Microbiology

Microfluidics: the propellant of CRISPR-based nucleic acid detection

Yanju Chen et al.

Summary: CRISPR/Cas systems have potential as nucleic acid detection tools, but their widespread application is limited due to low sensitivity and difficulties in multiplex detection. Microfluidics combined with CRISPR systems can improve detection ability, enabling fast, high-throughput, integrated, multiplex, and digital detection, further popularizing CRISPR in various scenarios.

TRENDS IN BIOTECHNOLOGY (2023)

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Article Biophysics

Multiplexed detection of foodborne pathogens using one-pot CRISPR/ Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic biosensor

Gaowa Xing et al.

Summary: In this study, a finger-actuated microfluidic biosensor was developed for rapid and sensitive detection of foodborne pathogens. The biosensor integrated one-pot CRISPR/Cas12a with recombinase aided amplification (RAA), and could be operated without the need for an external driving device. The results showed that the biosensor had high sensitivity and accuracy, making it suitable for point-of-care testing in resource-constrained settings.

BIOSENSORS & BIOELECTRONICS (2023)

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Article Engineering, Environmental

Argonaute-triggered visual and rebuilding-free foodborne pathogenic bacteria detection

Yaru Li et al.

Summary: Foodborne pathogenic bacteria are major causes of microbial contamination in food safety, and early screening and ultrasensitive detection are crucial for guaranteeing food safety. In this study, a Novel and One-step cleavage method based on Argonaute (NOTE-Ago) was developed, which allowed target nucleic acid to be cleverly converted into fluorescent signals. The NOTE-Ago assay exhibited a detection limit of 1 CFU/mL and a dynamic range from 1 to 108 CFU/mL, with satisfactory selectivity for detecting S. typhi and S. aureus contaminated food samples. This work enriches the toolbox of Argonaute-based detection and provides a one-step cleavage and rebuilding-free method for ultrasensitive bacteria detection.

JOURNAL OF HAZARDOUS MATERIALS (2023)

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Article Food Science & Technology

A CRISPR/dCas9-enabled, on-site, visual, and bimodal biosensing strategy for ultrasensitive and self-validating detection of foodborne pathogenic bacteria

Yaru Li et al.

Summary: We developed a biosensor called SCOUT-dCas9 for ultrasensitive detection of Salmonella typhimurium. This biosensor utilizes a combination of amplification, dCas9, and single guide RNA to convert the target into bimodal signals for cross-validation. SCOUT-dCas9 can detect as low as 1 CFU/mL of Salmonella typhimurium and demonstrates good selectivity.

FOOD FRONTIERS (2023)

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Article Biochemical Research Methods

Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a

Xiu Liu et al.

Summary: This study developed an electrochemical biosensor by coupling the CRISPR system with HCR for detecting Salmonella typhimurium. The interaction between DNA wires and CRISPR-Cas12a enabled the detection of electrochemical tags, allowing for selective and sensitive quantification of the pathogenic bacterium in samples. This method provides a novel insight for exploring universal analytical methods for pathogenic bacteria based on CRISPR-Cas12a coupled with HCR.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022)

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Article Food Science & Technology

A portable CRISPR Cas12a based lateral flow platform for sensitive detection of Staphylococcus aureus with double insurance

Jiajie Qian et al.

Summary: The article presents a portable and visible detection platform for S. aureus based on CRISPR/Cas12a which shows high sensitivity and specificity in pure culturing samples and artificially contaminated food samples. The platform combines ultra-sensitivity, specificity, portability, user-friendliness, and time-saving features, meeting the actual requirements for point-of-care testing and holding promise for further applications in detecting other foodborne pathogens.

FOOD CONTROL (2022)

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Article Agriculture, Multidisciplinary

Isothermal RNA Amplification for the Detection of Viable Pathogenic Bacteria to Estimate the Salmonella Virulence for Causing Enteritis

Ting Xue et al.

Summary: We reported an RNA detection method that allows for rapid detection of viable pathogenic bacteria within 2.5 hours. This method involves the direct amplification of 16S rRNA from viable Salmonella enterica and uses Cas13a/crRNA to ensure the specificity of amplification. Using this method, we found that S. enterica mainly colonizes the cecum, colon, and rectum in mice, and the severity of enteritis is determined by the number of viable S. enterica. Compared to qPCR, this method can accurately estimate the virulence of the pathogen, showing promise as a tool for monitoring pathogen contamination and biosafety control.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

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Article Immunology

Development and clinical implications of a novel CRISPR-based diagnostic test for pulmonary Aspergillus fumigatus infection

Zhengtu Li et al.

Summary: A highly sensitive and specific method for the detection of A. fumigatus using the CRISPR/Cas13a system has been developed. The method shows a promising potential for clinical diagnosis with high specificity and sensitivity.

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION (2022)

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Article Chemistry, Analytical

CRISPR/Cas12a based fluorescence-enhanced lateral flow biosensor for detection of Staphylococcus aureus

Baoqing Zhou et al.

Summary: The novel CRISPR/Cas12a-based fluorescence enhanced LFB combined with functionalized quantum dots and RAA allows for low-cost, simple, and sensitive detection of Staphylococcus aureus. This method shows high specificity and sensitivity in detecting the target pathogen within a short period of time, making it suitable for onsite testing in food and clinical diagnosis.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Biochemical Research Methods

Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay

Xiaoying Zhu et al.

Summary: This study introduced a CRISPR/Cas system for detecting transgenic crops, allowing label-free and direct recognition of transgenes through isothermal amplification. The use of Cas12a and rolling circle amplification enhanced the sensitivity of the assay, enabling specific detection of transgenes. The method, termed isoCRISPR, can distinguish transgenic corn cultivars from nontransgenic ones, enriching the toolbox for transgenic crop identification.

ACS SYNTHETIC BIOLOGY (2022)

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Article Chemistry, Multidisciplinary

CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens

Zhe Jiao et al.

Summary: A rapid, visual, and double-checked Logic Gate detection platform has been developed for the detection of pathogenic microorganisms using AIEgens in combination with CRISPR/Cas technology. The method allows for direct observation of emission changes with the naked eye and has strong specificity and speed, making it applicable for environmental water samples.

FRONTIERS IN CHEMISTRY (2022)

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Article Biotechnology & Applied Microbiology

CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

Yaoqiang Shi et al.

Summary: Researchers have developed a novel diagnostic test using a combination of LAMP and CRISPR/Cas12a to detect S. flexneri infection quickly and reliably, with results visible to the naked eye in 40 minutes.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Review Food Science & Technology

Upconversion luminescent nanomaterials: A promising new platform for food safety analysis

Deshmukh Abdul Hakeem et al.

Summary: Lanthanide ion-doped upconversion nanoparticles (UCNPs) have emerged as a cutting-edge platform in food safety research due to their superior physicochemical features. They are used for detection of foodborne pathogens and are also studied for synthesis strategies, luminescence, structure, morphology, and surface engineering. Further research is needed for the challenging and future applications of UCNPs in food safety research.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Biophysics

Engineered tracrRNA for enabling versatile CRISPR-dCas9-based biosensing concepts

Saba Safdar et al.

Summary: In recent years, CRISPR-Cas-based technologies have gained increasing attention in the biosensing field. Researchers have successfully integrated flexible signal generation and amplification properties into the CRISPR-dCas9 complex by incorporating a DNA sequence, allowing for the detection of nucleic acid targets.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Agriculture, Multidisciplinary

Ultrasensitive CRISPR/Cas12a-Driven SERS Biosensor for On-Site Nucleic Acid Detection and Its Application to Milk Authenticity Testing

Ruiyuan Pan et al.

Summary: This study proposes an ultrasensitive surface-enhanced Raman scattering (SERS) biosensor driven by CRISPR/Cas12a for on-site nucleic acid detection. The biosensor achieves precise target DNA recognition and signal amplification through specific base pairing and efficient trans-cleavage capability of CRISPR/Cas12a. The strategy exhibits an ultralow detection limit and is successfully applied to milk authenticity detection.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

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Article Chemistry, Analytical

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157:H7

So-Young Lee et al.

Summary: In this study, a rapid, sensitive, and visualized method of detecting E. coli O157:H7 in fresh products was developed, which rectified the common false-negative results and had a high detection sensitivity.

TALANTA (2022)

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Article Microbiology

Rapid and Ultrasensitive Detection of Methicillin-Resistant Staphylococcus aureus Based on CRISPR-Cas12a Combined With Recombinase-Aided Amplification

Ying Wang et al.

Summary: In this study, a method based on CRISPR-Cas12a and RAA was successfully proposed for accurate identification and highly sensitive detection of MRSA in clinical samples. The method showed high sensitivity and specificity, and provided important value for rapid detection of MRSA.

FRONTIERS IN MICROBIOLOGY (2022)

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Article Genetics & Heredity

An Inexpensive CRISPR-Based Point-of-Care Test for the Identification of Meat Species and Meat Products

Dagang Tao et al.

Summary: The growing demand for and supply of meat and meat products has led to an increase in cases of meat adulteration. Existing laboratory methods for meat species identification have limitations in terms of equipment and field applications. This study developed a cost-effective and rapid detection method that can be easily deployed in any standard room.

GENES (2022)

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Article Medicine, General & Internal

Rapid One-Tube RPA-CRISPR/Cas12 Detection Platform for Methicillin-Resistant Staphylococcus aureus

Yanan Li et al.

Summary: In this study, a rapid and accurate platform for MRSA detection was developed by integrating recombinase polymerase amplification (RPA) with the Cas12 system. The platform allows specific MRSA detection with high sensitivity and can visualize the results within 20 minutes.

DIAGNOSTICS (2022)

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Article Chemistry, Analytical

Immunocapture Magnetic Beads Enhanced the LAMP-CRISPR/Cas12a Method for the Sensitive, Specific, and Visual Detection of Campylobacter jejuni

Chao Li et al.

Summary: The study developed an all-in-one, simple, rapid, ultrasensitive, ultraspecific, and instrument-free visual detection method for Campylobacter jejuni. Based on ICB capture, LAMP reaction, and CRISPR/Cas12a detection, the method could detect very low concentrations of C. jejuni within a short period of time.

BIOSENSORS-BASEL (2022)

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Article Agriculture, Multidisciplinary

Sensitive Detection of Salmonella Based on CRISPR-Cas12a and the Tetrahedral DNA Nanostructure-Mediated Hyperbranched Hybridization Chain Reaction

Qiqi Cai et al.

Summary: A sensitive detection method for Salmonella was developed by coupling immunomagnetic separation with the CRISPR-Cas12a system and the tetrahedral DNA nanostructure-mediated hyperbranched hybridization chain reaction (TDN-hHCR). Salmonella as low as 8 CFU/mL could be easily detected, and it showed potential application in real samples.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

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Article Chemistry, Analytical

Ultrasensitive fluorescent biosensor for detecting CaMV 35S promoter with proximity extension mediated multiple cascade strand displacement amplification and CRISPR/Cpf 1

Yin Liu et al.

Summary: A novel fluorescent biosensor based on CRISPR/Cpf 1-mediated PE-MC/SDA-CRISPR/Cpf1 amplification reaction was proposed for detecting the CaMV 35S promoter in genetically modified organisms. The method exhibited high detection sensitivity, low background interference, and excellent selectivity.

ANALYTICA CHIMICA ACTA (2022)

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Article Biophysics

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Jianwen Zhuang et al.

Summary: In this study, a RPA-Cas12a-mu PAD was designed using CRISPR/Cas12a and SERS technology for highly sensitive bacterial detection. The concentration of S.typhi was determined by the aggregation level of SERS nanoprobes, enabling fast and accurate testing of food samples.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Food Science & Technology

CRISPR-Cas-based detection for food safety problems: Current status, challenges, and opportunities

Yaru Li et al.

Summary: Food safety is a critical global issue, and current detection methods have some shortcomings. As an emerging technology, the CRISPR-Cas system has great potential for applications in food safety detection, and it is expected to address the limitations of existing methods.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2022)

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Article Chemistry, Applied

Alkaline lysis-recombinase polymerase amplification combined with CRISPR/Cas12a assay for the ultrafast visual identification of pork in meat products

Gang Zhao et al.

Summary: The study successfully established a sensitive and rapid detection method for pig-derived components using CRISPR/Cas12a combined with recombinase polymerase amplification. The researchers obtained satisfactory results by testing beef and pork binary mixture models under different processing conditions, as well as combining with two different DNA extraction methods.

FOOD CHEMISTRY (2022)

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Article Agriculture, Multidisciplinary

Development of Recombinase-Aided Amplification (RAA)-Exo-Probe and RAA-CRISPR/Cas12a Assays for Rapid Detection of Campylobacter jejuni in Food Samples

Shuai Zhi et al.

Summary: A new RAA-exo-probe and RAA-CRISPR/Cas12a assays were developed for the rapid detection of Campylobacter jejuni in food samples. The assays showed high specificity and sensitivity, with a detection threshold lower than the traditional culture method. The simplicity and low equipment requirement make these assays suitable for field or poorly equipped labs.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

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Article Biotechnology & Applied Microbiology

Sensitive and high-accuracy detection of Salmonella based on CRISPR/Cas12a combined with recombinase polymerase amplification

X. Mao et al.

Summary: A simple, rapid, sensitive, and highly accurate detection technique for Salmonella based on CRISPR-Cas12a was developed, which can rapidly detect trace Salmonella through fluorescence intensity.

LETTERS IN APPLIED MICROBIOLOGY (2022)

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Article Microbiology

Development and evaluation of a rapid RPA/CRISPR-based detection of Francisella tularensis

Jian-Hao Xu et al.

Summary: Francisella tularensis is a dangerous pathogen that poses a severe threat to public health. In this study, a two-pronged rapid and ultrasensitive diagnostic method for detecting F. tularensis was developed using recombinase polymerase amplification (RPA) and CRISPR/Cas12a system. The assay showed high sensitivity and specificity, and could successfully detect F. tularensis in simulated blood and sewage samples.

FRONTIERS IN MICROBIOLOGY (2022)

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Article Food Science & Technology

Ultra-Sensitive and Rapid Detection of Pathogenic Yersinia enterocolitica Based on the CRISPR/Cas12a Nucleic Acid Identification Platform

Yiran Xiao et al.

Summary: A simple, visual, and ultrasensitive method based on CRISPR/Cas12a and RPA was developed for the detection of Yersinia enterocolitica. The method achieved a rapid detection of Y. enterocolitica in less than 45 minutes, with a detection limit 100 times lower than qPCR.

FOODS (2022)

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Article Food Science & Technology

CE-RAA-CRISPR Assay: A Rapid and Sensitive Method for Detecting Vibrio parahaemolyticus in Seafood

Xinrui Lv et al.

Summary: A system combining chemically enhanced CRISPR and RAA technologies was established for detecting V. parahaemolyticus in seafood, showing rapid, accurate detection with no cross-reactivity.

FOODS (2022)

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Article Chemistry, Analytical

Aptamer-based colorimetric detection of methicillin-resistant Staphylococcus aureus by using a CRISPR/Cas12a system and recombinase polymerase amplification br

Luyu Wei et al.

Summary: Aptamer-based colorimetric biosensor using CRISPR/Cas12a and RPA was developed for sensitive and convenient detection of MRSA. The biosensor integrated the CRISPR/Cas12a system with colorimetry by employing an aptamer that served as the substrate and modulator. Multiple amplification strategies and signal amplification were employed to enhance the sensitivity, and the biosensor exhibited high accuracy and simplicity in detecting MRSA in real samples, suggesting its potential as a robust antibiotic-resistant bacteria detection platform.

ANALYTICA CHIMICA ACTA (2022)

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Review Biophysics

Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Xinkuan Cheng et al.

Summary: CRISPR/Cas tools have shown great potential in biosensing beyond gene editing. However, the application of CRISPR/Cas-based biosensors for non-nucleic acid targets is still in its early stages. This review summarizes the strategies and prospects of CRISPR/Cas toolboxes in recognizing non-nucleic acid targets, providing valuable information for the expansion of these powerful tools into multiple detection fields.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Biophysics

DropCRISPR: A LAMP-Cas12a based digital method for ultrasensitive detection of nucleic acid

Hui Wu et al.

Summary: This study reports a digital droplet-based platform (DropCRISPR) that combines loop-mediated isothermal amplification (LAMP) with CRISPR/Cas12a technology to achieve ultrasensitive and quantitative detection of nucleic acids. The method utilizes a novel microfluidic system to inject CRISPR/Cas12a reagents into each droplet, overcoming temperature incompatibilities and interference between LAMP and CRISPR/Cas12a.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Food Science & Technology

CRISPR-Cas systems mediated biosensing and applications in food safety detection

Jianghua Liu et al.

Summary: Food safety is a global concern that impacts the economic development of the food industry and public health. The emerging CRISPR-Cas systems show promise for addressing the limitations of classical detection methods and developing next-generation techniques. This review focuses on the recent progress of CRISPR-Cas mediated biosensing in food safety monitoring, highlighting the properties and principles of commonly used systems, amplification strategies, and signal output modes. Furthermore, it comprehensively describes the application of CRISPR-Cas systems-based biosensors in detecting various food safety hazards and discusses the current challenges and future prospects in this field.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Food Science & Technology

The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops

Jinbin Wang et al.

Summary: An ultra-sensitive visualization detection method based on RPA-Cas12a-LFB was developed for rapid detection of genetically modified crops. The method demonstrated high sensitivity, specificity, and feasibility, with a short detection time.

FOOD CONTROL (2022)

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Article Agriculture, Multidisciplinary

CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection

Jiahui Chen et al.

Summary: In this study, a precisely regulated DNA-templated silver nanocluster (DNA-AgNC) sensor based on CRISPR/Cas12a was designed for sensitive detection of meat adulteration. The strategy achieved a satisfactory linear range and a low limit of detection, providing an accurate and efficient method for monitoring meat authenticity.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

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Article Chemistry, Analytical

Sensitive and Rapid Detection of Escherichia coli O157:H7 From Beef Samples Based on Recombinase Aided Amplification Assisted CRISPR/Cas12a System

Taisong Fang et al.

Summary: A recombinase aided amplification (RAA) assisted CRISPR/Cas12a (RAA-CRISPR/Cas12a) fluorescence platform was developed for specific, sensitive, and rapid nucleic acid detection of E. coli O157:H7. The method showed high specificity and sensitivity, and the detection could be completed within 30 min.

JOURNAL OF AOAC INTERNATIONAL (2022)

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Article Chemistry, Physical

A cascade amplification strategy for ultrasensitive Salmonella typhimurium detection based on DNA walker coupling with CRISPR-Cas12a

Haijiao Zhang et al.

Summary: In this study, a novel detection machinery based on DNA walker and CRISPR-Cas12a technologies was developed for highly sensitive detection of Salmonella typhimurium. The cascade amplification strategy exhibited excellent specificity and significantly reduced the limit of detection of DNA walker.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2022)

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Article Food Science & Technology

CRISPR Cas12a-based sweet biosensor coupled with personal glucose meter readout for the point-of-care testing of Salmonella

Chi Zhou et al.

Summary: A sensitive, rapid, user-friendly, and quantitative detection method for Salmonella in food based on CRISPR Cas12a and personal glucose meter (PGMs) has been developed in this study, which is of great significance to food safety supervision and management.

JOURNAL OF FOOD SCIENCE (2022)

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Article Environmental Sciences

Generation and application of a novel high-throughput detection based on RPA-CRISPR technique to sensitively monitor pathogenic microorganisms in the environment

Li Liu et al.

Summary: In this study, a high-throughput and highly sensitive method for monitoring Staphylococcus aureus in water using the RPA-CRISPR/Cas12a detection system was developed. This method combines nucleic acid amplification and the activity of the CRISPR/Cas12a system to achieve rapid and accurate detection of S. aureus in water samples within 35 minutes.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

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Article Virology

Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay

Weidong Qian et al.

Summary: This study developed a low-cost, disposable, and ultrasensitive assay for diagnosing human pathogens through the integration of CRISPR-Cas12a sensors with isothermal signal amplification. The assay successfully detected the GII.4 and GII.17 genotypes of norovirus (NOV) with high specificity and a low limit of detection. The method provided visual results and a faster alternative to real-time RT-PCR, with reliable accuracy.

VIROLOGY JOURNAL (2022)

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Article Microbiology

A novel detection method for the pathogenic Aeromonas hydrophila expressing aerA gene and/or hlyA gene based on dualplex RAA and CRISPR/Cas12a

Ziqin Lin et al.

Summary: In this study, a rapid, accurate, sensitive, and visual detection method for pathogenic A. hydrophila was developed using the dRAA-CRISPR/Cas12a system. The method showed high sensitivity and specificity for detecting A. hydrophila strains expressing specific virulence genes. It also demonstrated satisfactory practicability in analyzing spiked human blood and stool samples, as well as fish samples.

FRONTIERS IN MICROBIOLOGY (2022)

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Review Food Science & Technology

CRISPR-Cas based molecular diagnostics for foodborne pathogens

Yunhao Lu et al.

Summary: Foodborne pathogenic infections have caused various problems in human life, and there is a need for advanced detection technologies. CRISPR/Cas-based biosensors have the potential to address the challenges of conventional assays such as insensitivity, long turnaround time, and complex pretreatments. This review focuses on the strategies of CRISPR/Cas-assisted diagnostics for foodborne pathogens, specifically discussing biosensing platforms based on fluorescence, colorimetric, (electro)chemiluminescence, electrochemical, and surface-enhanced Raman scattering detection. The review summarizes the detection principles for foodborne pathogenic bacteria, fungi, and viruses. The current challenges and technical barriers of these methods in broad application are also discussed, along with alternative solutions to improve the potential of CRISPR/Cas for food safety.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Parasitology

A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)

Jinhong Zhao et al.

Summary: This study developed a new method for rapid visual detection of Toxoplasma gondii infection using recombinase-aided amplification (RAA) and lateral flow dipstick (LFD) coupled with CRISPR-Cas13a fluorescence. The method is not only rapid, sensitive, and specific, but also allows direct visualization by the naked eye, eliminating the need for sophisticated and costly equipment. It can be applied to on-site surveillance of T. gondii.

PARASITE (2022)

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Article Chemistry, Analytical

The fluorescent biosensor for detecting N6 methyladenine FzD5 mRNA and MazF activity

Gaihua Cao et al.

Summary: An ultra-sensitive biosensor based on MazF, cascaded strand displacement amplification (CSDA) and CRISPR/Cas12a was developed for the detection of m(6)A FzD5 mRNA, showing excellent sensitivity and detection limit.

ANALYTICA CHIMICA ACTA (2021)

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Article Chemistry, Analytical

Label-Free Colorimetric Method for Detection of Vibrio parahaemolyticus by Trimming the G-Quadruplex DNAzyme with CRISPR/Cas12a

Xueyun Chen et al.

Summary: A novel label-free, colorimetric method for visual detection of V. parahaemolyticus was proposed, utilizing CRISPR/Cas12a to trim G-quadruplex DNAzyme and prevent color development of ABTS(2-). The method showed sensitivity comparable to real-time LAMP, detecting 6.1 x 10^2 CFU/mL V. parahaemolyticus in shrimp samples, making it a promising universal biosensing strategy for field pathogenic bacterial testing.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Cas12aFDet: A CRISPR/Cas12a-based fluorescence platform for sensitive and specific detection of Listeria monocytogenes serotype 4c

Fan Li et al.

Summary: The study introduces a CRISPR/Cas12a-based fluorescence detection platform called Cas12aFDet for rapid nucleic acid detection, which overcomes limitations of existing methods such as time consumption and contamination risk. The platform demonstrates high sensitivity and specificity for detecting Listeria strains in both pure culture and natural samples.

ANALYTICA CHIMICA ACTA (2021)

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Article Chemistry, Analytical

Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction

Mahera J. Kachwala et al.

Summary: Hybridization chain reaction (HCR) is a DNA-based target-induced cascade reaction that is often used for sensing applications due to its unique enzyme-free amplification feature. HCR is highly modular and can be advanced and repurposed when coupled with latest discoveries for improved sensitivity and programmability. Incorporating CRISPR-Cas12a into HCR can enhance sensitivity and enable rapid reprogramming of detection assays, making it more versatile for detecting different target sequences.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

CRISPR-Cas based virus detection: Recent advances and perspectives

Lijuan Yin et al.

Summary: This article highlights the recent advances in virus detection using CRISPR-Cas systems, particularly CRISPR-Cas12a/Cas13a. These systems, known for their sensitivity, specificity, high base resolution, and programmability, are revolutionizing virus detection and offering new possibilities in the field.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Applied

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Hua Liu et al.

Summary: This study developed a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety, which can rapidly, simply, and highly sensitively detect foodborne pathogenic bacteria, genetically modified crops, and meat adulteration without requiring technical expertise or equipment.

FOOD CHEMISTRY (2021)

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Article Chemistry, Analytical

CRISPR/Cas12a and immuno-RCA based electrochemical biosensor for detecting pathogenic bacteria

Zhibao Chen et al.

Summary: An electrochemical biosensor based on CRISPR/Cas12a and immuno-RCA was developed for rapid, simple, and sensitive detection of food-borne pathogenic bacterium Escherichia coli O157:H7. The biosensor showed a wide dynamic detection range and low detection limit, without cross-reactivity with other non-target bacteria.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY (2021)

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Article Food Science & Technology

An ultrasensitive and contamination-free on-site nucleic acid detection platform for Listeria monocytogenes based on the CRISPR-Cas12a system combined with recombinase polymerase amplification

Yachen Tian et al.

Summary: The Cas12a-MPR method combines CRISPR-Cas12a and RPA technologies to achieve rapid, accurate, and highly sensitive detection of foodborne pathogens in the same vessel. The research demonstrates that this method can specifically detect target DNA at the attomolar level and achieve a detection limit of 10 CFU mL-1 at 37 degrees Celsius, with 100% accuracy in detecting Listeria monocytogenes contamination.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

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Article Chemistry, Analytical

Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay

Lijuan Yin et al.

Summary: A G-quadruplex-based CRISPR-Cas12a bioassay was developed for high sensitivity and visualization detection of Salmonella. The method achieved a detection limit of 1 CFU/mL for Salmonella and successfully detected the bacteria in real food samples.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Chemistry, Analytical

Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

Min Qing et al.

Summary: The study introduced the CRISPR/Cas system into an electrochemical biosensing platform for sensitive and specific detection of disease-related molecules. By utilizing modular rolling circle amplification and CRISPR/Cas12a, signal amplification was achieved. The flexibility and programmability of this strategy allow for the detection of various targets.

ANALYTICAL CHEMISTRY (2021)

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Article Microbiology

Rapid and Sensitive Detection of Vibrio vulnificus Using CRISPR/Cas12a Combined With a Recombinase-Aided Amplification Assay

Xingxing Xiao et al.

Summary: A rapid and sensitive diagnostic method, RAA-CRISPR/Cas12a, has been developed for detecting V. vulnificus, showing great potential for early diagnosis of human vibriosis and on-site detection in aquaculture and food safety control.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Biophysics

A reversible valve-assisted chip coupling with integrated sample treatment and CRISPR/Cas12a for visual detection of Vibrio parahaemolyticus

Hui Wu et al.

Summary: A rapid and low-cost detection method for V. parahaemolyticus using a chip and CRISPR/Cas12a was established, allowing for detection within 50 minutes with high sensitivity. The method showed great feasibility for real samples detection and has potential applications in food safety analysis and clinical diagnostics, especially in resource-limited areas.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Microbiology

Combining CRISPR-Cas12a-Based Technology and Metagenomics Next Generation Sequencing: A New Paradigm for Rapid and Full-Scale Detection of Microbes in Infectious Diabetic Foot Samples

Yixin Chen et al.

Summary: The CRISPR-LAMP assay developed in this study successfully detected Staphylococcus aureus strains and differentiated MRSA. The specificity of the LAMP-CRISPR assay for nuc reached 100%, without cross-reactions with non-S. aureus strains. The assay showed 94.4% agreement with clinical culture results in evaluating 18 samples from DFI patients.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Biophysics

Carrying out pseudo dual nucleic acid detection from sample to visual result in a polypropylene bag with CRISPR/Cas12a

Hui Wu et al.

Summary: The polypropylene (PP) bag-based method for nucleic acid detection offers the potential for at-home testing, with the ability to visually detect Salmonella typhimurium (St) and SARS-CoV-2 within 1 hour; the combination of magnetic particles and isothermal amplification technology allows for convenient processing. Introducing a logic decision method enhances the reliability of at-home nucleic acid testing.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for Listeria monocytogenes detection

Fan Li et al.

Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Microbiology

Rapid and Sensitive Detection of Salmonella spp. Using CRISPR-Cas13a Combined With Recombinase Polymerase Amplification

Bailin An et al.

Summary: The study developed a simple, rapid, and specific detection method for Salmonella spp. using one-tube RPA-Cas13a, while the two-step assay was more sensitive, particularly for samples at low abundance.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Food Science & Technology

Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system

Shujuan Wang et al.

Summary: The CRISPR-based diagnostic method utilizing the rfbE gene in the CRISPR/Cas12a system enables rapid detection of Escherichia coli O157:H7 with high sensitivity and specificity, with low detection limits for both DNA and bacterial concentrations. The target was successfully detected in ground beef samples after 4 hours of culture using Metal Organic Framework immunomagnetic beads enrichment when the initial bacterial inoculum was 14 CFU/mL.

FOOD CONTROL (2021)

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Article Chemistry, Multidisciplinary

Rotary Valve-Assisted Fluidic System Coupling with CRISPR/Cas12a for Fully Integrated Nucleic Acid Detection

Hui Wu et al.

Summary: A fully integrated nucleic acid detection system was established using a fluidic chip coupled with CRISPR/Cas12a, which achieved high sensitivity in nucleic acid extraction, amplification, and detection.

ACS SENSORS (2021)

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Review Chemistry, Analytical

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

Si-Yuan Wang et al.

Summary: CRISPR/Cas proteins are powerful gene-editing tools and also show great promise in biosensing applications. Various nucleic acid-based signal amplification techniques can greatly improve detection sensitivity and specificity, extend the detectable target range, and enable diverse signal output modes in CRISPR/Cas-based biosensors.

ANALYTICA CHIMICA ACTA (2021)

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Article Chemistry, Analytical

A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles

Khalil Abnous et al.

Summary: A highly sensitive colorimetric aptasensor was developed for the detection of AFM(1) using CRISPR-Cas12a, RCA, and AuNPs. The sensor showed high selectivity towards AFM(1) with a detection limit as low as 0.05 ng/L, and was able to sensitively identify AFM(1) in spiked milk samples.

ANALYTICA CHIMICA ACTA (2021)

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Review Biochemistry & Molecular Biology

Isothermal amplifications - a comprehensive review on current methods

Jorn Gloekler et al.

Summary: The introduction of nucleic acid amplification techniques, particularly isothermal amplification methods, has revolutionized the field of medical diagnostics by simplifying nucleic acid amplification and impacting the design of molecular diagnostics. Despite the advantages they bring, the heterogeneous issues and complexities surrounding different isothermal amplification methods make it challenging to identify the best approach.

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY (2021)

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Article Food Science & Technology

Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

Jiajia Wan et al.

Summary: Two real-time quantitative PCR detection systems were developed for detecting Salmonella Enteritidis and Salmonella spp. using DNA Green and Ly Green saturated fluorescent dyes. The specificity of the systems was improved by adding graphene quantum dots, with high R2 values for standard curves and evaluations of sensitivity for different aspects.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

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Article Chemistry, Multidisciplinary

CRISPR-Cas12a-Powered Dual-Mode Biosensor for Ultrasensitive and Cross-validating Detection of Pathogenic Bacteria

Long Ma et al.

Summary: The study proposed a CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria, with satisfactory selectivity for Salmonella and capabilities in food sample detection. The technology expands the reach of CRISPR-Cas system in biosensing and provides a general method for bacteria sensing with desirable sensitivity, specificity, and cross-validating capacity.

ACS SENSORS (2021)

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Article Chemistry, Multidisciplinary

G-Quadruplex-Probing CRISPR-Cas12 Assay for Label-Free Analysis of Foodborne Pathogens and Their Colonization In Vivo

Xuhan Xia et al.

Summary: The study developed a label-free detection method based on the G-CRISPR-Cas system, allowing highly sensitive detection of foodborne pathogen Salmonella enterica and investigation of their colonization in chickens.

ACS SENSORS (2021)

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Article Microbiology

RAA-Cas12a-Tg: A Nucleic Acid Detection System for Toxoplasma gondii Based on CRISPR-Cas12a Combined with Recombinase-Aided Amplification (RAA)

Qiao-Ni Ma et al.

Summary: Toxoplasmosis, caused by Toxoplasma gondii, is a significant parasitic zoonosis globally. The newly developed RAA-Cas12a-Tg system showed high sensitivity and specificity in detecting T. gondii oocysts in soil samples within an hour, providing a rapid and efficient method for on-site detection of the parasite.

MICROORGANISMS (2021)

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Article Food Science & Technology

Double Isothermal Amplification and CRISPR-Cas12a for Sensitive Detection of Citrinin

Man Zhang et al.

Summary: An analytical method using double isothermal amplification and CRISPR-Cas12a has been developed for ultrasensitive detection of citrinin. This method combines gold nanoparticles modified with antigen and thiol-terminated, single-strand DNA as a probe, magnetic bead separation, and fluorescence signal generation to achieve high sensitivity and selectivity in toxin detection in food.

ACS FOOD SCIENCE & TECHNOLOGY (2021)

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Article Chemistry, Analytical

Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive

Hui Wu et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Chemistry, Analytical

CRISPR-Cas9 Triggered Two-Step Isothermal Amplification Method for E. coli O157:H7 Detection Based on a Metal-Organic Framework Platform

Xuan Sun et al.

ANALYTICAL CHEMISTRY (2020)

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Review Cell Biology

The rapidly advancing Class 2 CRISPR-Cas technologies: A customizable toolbox for molecular manipulations

Jingyi Wang et al.

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)

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Article Chemistry, Analytical

Integration of logic gates to CRISPR/Cas12a system for rapid and sensitive detection of pathogenic bacterial genes

Lei Peng et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Chemistry, Multidisciplinary

A One-Pot Toolbox Based on Cas12a/crRNA Enables Rapid Foodborne Pathogen Detection at Attomolar Level

Yunqing Wang et al.

ACS SENSORS (2020)

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Article Chemistry, Analytical

CRISPR-Cas13a based bacterial detection platform: Sensing pathogen Staphylococcus aureus in food samples

Jin Zhou et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Biophysics

An ultrasensitive and specific point-of-care CRISPR/Cas12 based lateral flow biosensor for the rapid detection of nucleic acids

Omar Mukama et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Biophysics

End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor

Hui Wu et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Food Science & Technology

Genetic authentication: Differentiation of fine and bulk cocoa (Theobroma cacao L.) by a new CRISPR/Cas9-based in vitro method

Alexandra Scharf et al.

FOOD CONTROL (2020)

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Article Biochemical Research Methods

Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplification

Liqi Xu et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2020)

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Article Chemistry, Analytical

Reagents-Loaded, Automated Assay that Integrates Recombinase-Aided Amplification and Cas12a Nucleic Acid Detection for a Point-of-Care Test

Yong Chen et al.

ANALYTICAL CHEMISTRY (2020)

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Article Biochemical Research Methods

CRISPR-Cas12a-Assisted Multicolor Biosensor for Semiquantitative Point-of-Use Testing of the Nopaline Synthase Terminator in Genetically Modified Crops by Unaided Eyes

Di Huang et al.

ACS SYNTHETIC BIOLOGY (2020)

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Article Chemistry, Analytical

Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection

Bei Wang et al.

ANALYTICAL CHEMISTRY (2019)

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Article Biochemistry & Molecular Biology

RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA

Amir Abdolahzadeh et al.

RNA (2019)

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Article Biochemistry & Molecular Biology

Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y. Wu et al.

NATURE CHEMICAL BIOLOGY (2018)

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Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

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Article Multidisciplinary Sciences

A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

Wenhua Zhou et al.

NATURE COMMUNICATIONS (2018)

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Review Biochemistry & Molecular Biology

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit

Karthik Murugan et al.

MOLECULAR CELL (2017)

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Article Multidisciplinary Sciences

Hinge-initiated Primer-dependent Amplification of Nucleic Acids (HIP) - A New Versatile Isothermal Amplification Method

Jens Fischbach et al.

SCIENTIFIC REPORTS (2017)

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Article Chemistry, Analytical

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

Mengqi Huang et al.

ANALYTICAL CHEMISTRY (2017)

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Article Biophysics

A facile, rapid and sensitive detection of MRSA using a CRISPR-mediated DNA FISH method, antibody-like dCas9/sgRNA complex

Kyeonghye Guk et al.

BIOSENSORS & BIOELECTRONICS (2017)

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Article Biochemistry & Molecular Biology

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

Keith Pardee et al.

CELL (2016)

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Review Microbiology

Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Tsugunori Notomi et al.

JOURNAL OF MICROBIOLOGY (2015)

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Review Multidisciplinary Sciences

CRISPR-Cas immunity in prokaryotes

Luciano A. Marraffini

NATURE (2015)

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Article Multidisciplinary Sciences

Polymerase Spiral Reaction (PSR): A novel isothermal nucleic acid amplification method

Wei Liu et al.

SCIENTIFIC REPORTS (2015)

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Article Multidisciplinary Sciences

Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte

Mark J. Hoser et al.

PLOS ONE (2014)

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Article Biochemistry & Molecular Biology

Rapid detection of microbial DNA by a novel isothermal genome exponential amplification reaction (GEAR) assay

Jothikumar Prithiviraj et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2012)

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Article Multidisciplinary Sciences

Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification

Gaolian Xu et al.

SCIENTIFIC REPORTS (2012)

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Article Biochemical Research Methods

Self-dissociative primers for nucleic acid amplification and detection based on DNA quadruplexes with intrinsic fluorescence

Besik I. Kankia

ANALYTICAL BIOCHEMISTRY (2011)

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Article Biochemistry & Molecular Biology

Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods

Bingling Li et al.

NUCLEIC ACIDS RESEARCH (2011)

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Article Chemistry, Analytical

Isothermal Target and Signaling Probe Amplification Method, Based on a Combination of an Isothermal Chain Amplification Technique and a Fluorescence Resonance Energy Transfer Cycling Probe Technology

Cheulhee Jung et al.

ANALYTICAL CHEMISTRY (2010)

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Article Microbiology

Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens

Rendong Fang et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2009)

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Article Multidisciplinary Sciences

Programming biomolecular self-assembly pathways

Peng Yin et al.

NATURE (2008)

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Article Biochemistry & Molecular Biology

Highly efficient isothermal DNA amplification system using three elements of 5′-DNA-RNA-3′ chimeric primers, RNaseH and strand-displacing DNA polymerase

Hiroyuki Mukai et al.

JOURNAL OF BIOCHEMISTRY (2007)

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Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

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Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

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Article Biochemistry & Molecular Biology

Helicase-dependent isothermal DNA amplification

M Vincent et al.

EMBO REPORTS (2004)

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Article Multidisciplinary Sciences

Triggered amplification by hybridization chain reaction

RM Dirks et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

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Article Multidisciplinary Sciences

Isothermal reactions for the amplification of oligonucleotides

J Van Ness et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

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Article Biotechnology & Applied Microbiology

Ramification amplification: A novel isothermal DNA amplification method

DY Zhang et al.

MOLECULAR DIAGNOSIS (2001)

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Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)

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