Seroprevalence and associated risk factors for Toxoplasma gondii in water buffaloes (Bubalus bubalis) in Egypt

Toxoplasmosis is a parasitic disease caused by Toxoplasma gondii and affecting all warm-blooded animals. The available data about the epidemiological situation of T. gondii in water buffaloes in Egypt are scarce. Thus, a cross-sectional study was conducted to determine the seroprevalence of T. gondii in water buffaloes in three Egyptian governorates and to evaluate the associated risk factors for the infection. A total of 430 sera samples were examined using commercial Indirect ELISA Multi-species kit. The overall seroprevalence rate of T. gondii in examined water buffaloes was 7.4 %, and the highest rate (9.3 %) was found in Kafr ElSheikh governorate. Multivariate logistic regression analysis showed that adult buffalo (OR = 7.10; 95 % CI: 0.87-57.68; P = 0.067) and small herds (OR = 8.42; 95 % CI: 1.07-66.02; P = 0.043) were more likely than young buffalo and large herds to become infected with T. gondii. Moreover, the risk of buffaloes contracting T. gondii infection was higher in winter and especially among animals contacted with cats. It is necessary to identify risk factors in order to determine what mitigation, control, and prevention strategies to implement in order to reduce, control, and prevent T. gondii infection in domestic animals, which will in turn reduce human infection with the disease.

Automated summary

This study investigated the seroprevalence of Toxoplasma gondii infection in water buffaloes in Egypt and found that age and herd size of the buffaloes were associated with the infection risk. Additionally, winter season and contact with cats were identified as higher risk factors for the buffaloes to contract T. gondii infection. It is important to identify and address these risk factors to reduce and control the infection in buffaloes and subsequently decrease the risk of human infection.