期刊
CLINICA CHIMICA ACTA
卷 548, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.cca.2023.117530
关键词
Asthenozoospermia; Metabolomics; Seminal plasma; Gas chromatography-mass spectrometry
By analyzing the metabolic profiles of four different types of asthenozoospermia, this study revealed abnormal changes in metabolites and metabolic pathways associated with the disease. The findings provide new clues and evidence for the etiology, diagnosis, and treatment of asthenozoospermia.
Background: Asthenozoospermia (AZS) is a disease characterized by decreased sperm motility induced by multiple etiologies, and the pathological mechanisms of various AZS are unclear. We simultaneously analyzed the metabolic profiling of four representative AZS to provide new insights into the etiologies of AZS. Method: Seminal plasma samples were collected from healthy control (HC; n = 30) and four AZS induced by varicocele (VA, n = 30), obesity (OA, n = 22), reproductive system infections (RA; n = 17) and idiopathic (IA, n = 30), respectively, and were analyzed using gas chromatography-mass spectrometry. Disturbed metabolites and metabolic pathways were compared between AZS and HC, as well as IA and the other three AZS. Results: A total of 40 different metabolites were identified in the seminal plasma of AZS and HC, of which lactic acid, fructose, citric acid, glutamine and pyruvic acid metabolic abnormalities associated with all the AZS groups, while each AZS group had unique metabolic changes. RA was significantly separated from the other three AZS, and metabolites such as cholesterol, octadecanoic acid and serine mainly contributed to the separation. Conclusion: The comprehensive metabolomic analysis and comparison of four various AZS provided evidence and clues for the mechanism mining, which will benefit future etiology, diagnosis and treatment of AZS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据