期刊
CHEMISTRY & BIODIVERSITY
卷 -, 期 -, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbdv.202300893
关键词
antitumor; cisplatin; combinations; cytotoxic; triterpene; triterpenes
The combination of AMD or 3 alpha OH-AMD acids with CDDP exhibited increased cytotoxic effect in PC3 prostate and HCT116 colon cancer cells, but showed no additional activity in in vivo tests. Furthermore, the combinations of 3 alpha OH-AMD and CDDP exerted antagonistic effects in both cell types.
The cytotoxic activity of combinations of masticadienonic (AMD) or 3 alpha OH-hydroxy-masticadienonic (3 alpha OH-AMD) acids with cisplatin (CDDP) was evaluated against PC3 prostate and HCT116 colon cancer cell lines. Combinations A (half the IC50 value), B (IC50 value), and C (twice the IC50 value) were tested at a 1: 1 ratio. All AMD plus CDDP combinations demonstrated increased cytotoxic effect, as determined by the sulforhodamine B test, in both cell types. The best combination was B, which showed 93% and 91% inhibition of the proliferation of PC3 and HCT116 cells, respectively. It also increased apoptosis in the PC3 cell lines, as evaluated by flow cytometry. However, in vivo tests showed no additional activity from the AMD plus CDDP combinations. These results showed that the increased cytotoxic activity of the combinations in vitro did not reflect in vivo tests. All combinations of 3 alpha OH-AMD plus CDDP exerted antagonistic effects in both cell types.
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