Antibiofilm Activity of Invasive Plants against Candida albicans: Focus on Baccharis halimifolia Essential Oil and Its Compounds

The extracts of five invasive plants were investigated for antifungal and antibiofilm activities against Candida albicans, C. glabrata, C. krusei, and C. parapsilosis. The antifungal activity was evaluated using the microdilution assay and the antibiofilm effect by measurement of the metabolic activity. Ethanol and ethanol-water extracts of Reynoutria japonica leaves inhibited 50 % of planktonic cells at 250 & mu;g mL(-1) and 15.6 & mu;g mL(-1), respectively. Ethanol and ethanol-water extracts of Baccharis halimifolia inhibited >75 % of the mature biofilm of C. albicans at 500 & mu;g mL(-1). The essential oil (EO) of B. halimifolia leaves was the most active (50 % inhibition (IC50) at 4 and 74 & mu;g mL(-1)against the maturation phase and 24 h old-biofilms of C. albicans, respectively). Oxygenated sesquiterpenes were the primary contents in this EO (62.02 %), with & beta;-caryophyllene oxide as the major component (37 %). Aromadendrene oxide-(2), & beta;-caryophyllene oxide, and (& PLUSMN;)-& beta;-pinene displayed significant activities against the maturation phase (IC50=9-310 & mu; mol l(-1)) and preformed 24 h-biofilm (IC50=38-630 & mu; mol l(-1)) of C. albicans with very low cytotoxicity for the first two compounds. C. albicans remained the most susceptible species to this EO and its components. This study highlighted for the first time the antibiofilm potential of B. halimifolia, its EO and some of its components.

Automated summary

The extracts of five invasive plants were investigated for their antifungal and antibiofilm activities against various Candida species. The study found that the ethanol and ethanol-water extracts of Reynoutria japonica leaves and Baccharis halimifolia effectively inhibited the growth of Candida albicans and the formation of its biofilm. The essential oil of B. halimifolia leaves, particularly the compound β-caryophyllene oxide, showed the strongest activity against mature biofilms of C. albicans. This study highlighted the antibiofilm potential of B. halimifolia and its essential oil.