期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 385, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2023.110730
关键词
Tetracaine; Voltage-gated sodium channels; Matrix metalloproteinases; Invasion; Breast cancer
Tetracaine, a type of long-acting amino ester local anesthetic, can inhibit the invasive characteristics of breast cancer cells by suppressing their migratory, invasive, and adhesive abilities. This effect may be achieved by reducing the expression and activity of MMP-2 and MMP-9, as well as increasing TIMP-2 expression. The anti-invasive effects of tetracaine make it a potential candidate for clinical use in surgery and combination therapies.
Tetracaine, a long-acting amino ester-type local anesthetic, prevents the initiation and propagation of action potentials by reversibly blocking voltage-gated sodium channels (VGSCs). These channels, which are highly expressed in several carcinomas (e.g. breast, prostate, colon and lung cancers) have been implicated in promoting metastatic behaviours. Recent evidence suggests that local anesthetics can suppress cancer progression. In this paper, we aimed to explore whether tetracaine would reduce the invasive characteristics of breast cancer cells. In a comparative approach, we used two cell lines of contracting metastatic potential: MDA-MB-231 (strongly metastatic) and MCF-7 (weakly metastatic). Tetracaine (50 mu M and 75 mu M) did not affect the proliferation of both MDA-MB-231 and MCF-7 cells. Importantly, tetracaine suppressed the migratory, invasive, and adhesive capacities of MDA-MB-231 cells; there was no effect on the motility of MCF-7 cells. Tetracaine treatment also significantly decreased the expression and activity levels of MMP-2 and MMP-9, whilst increasing TIMP-2 expression in MDA-MB-231 cells. On the other hand, VGSC alpha/Nav1.5 and VGSC-beta 1 mRNA and protein expression levels were not affected. We conclude that tetracaine has anti-invasive effects on breast cancer cells and may be exploited clinically, for example, in surgery and/or in combination therapies.
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