☆ 4.5 Article

A Multimodal Omics Framework to Empower Target Discovery for Cardiovascular Regeneration

CARDIOVASCULAR DRUGS AND THERAPY (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Editorial Material Cardiac & Cardiovascular Systems

Scientists Identify Molecular Alterations in Failing Hearts at Single-Cell Resolution

Tracy Hampton

CIRCULATION (2023)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Multi-species meta-analysis identifies transcriptional signatures associated with cardiac endothelial responses in the ischaemic heart

Ziwen Li et al.

Summary: We present a high-resolution single-cell meta-atlas of healthy and injured coronary endothelial cells in the mouse and human heart, revealing a suite of novel targets with great potential to promote vascular regeneration, and providing a rich resource for therapeutic development.

CARDIOVASCULAR RESEARCH (2023)

添加到收藏夹

Article Cell Biology

Single-nucleus RNA sequencing in ischemic cardiomyopathy reveals common transcriptional profile underlying end-stage heart failure

Bridget Simonson et al.

Summary: Using single-nucleus RNA sequencing and computational analysis, the cellular and molecular signature of ischemic cardiomyopathy (ICM) was investigated. The study revealed alterations in the cellular composition of the ischemic heart, with decreased cardiomyocytes and increased proportions of lymphatic, angiogenic, and arterial endothelial cells in ICM patients. Furthermore, increased LAMININ signaling from endothelial cells and similarities in transcriptional changes were observed between ICM, hypertrophic, and dilated cardiomyopathies, which may help identify potential drug targets for end-stage heart failure.

CELL REPORTS (2023)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Direct Reprogramming Improves Cardiac Function and Reverses Fibrosis in Chronic Myocardial Infarction

Hidenori Tani et al.

Summary: By conducting experiments on mice, this study found that cardiac reprogramming can repair chronic myocardial infarction, promote myocardial regeneration, and reduce fibrosis. The results of this study provide new insights for the treatment of chronic myocardial infarction and heart failure.

CIRCULATION (2023)

添加到收藏夹

Article Developmental Biology

Single cell evaluation of endocardial Hand2 gene regulatory networks reveals HAND2-dependent pathways that impact cardiac morphogenesis

Rajani M. George et al.

Summary: The transcription factor HAND2 is crucial in cardiogenesis and its deletion causes various heart defects. Single cell transcriptome analysis of H2CKO hearts revealed disrupted endocardial regulatory pathways. HAND2-dependent enhancers, including two for KLF2, a master regulator of shear-stress, were identified using HAND2 DNA occupancy data. Deletion of a specific enhancer caused reduced Klf2 expression in ventricular endocardium, revealing HAND2's role in endocardial gene regulatory networks and shear-stress response.

DEVELOPMENT (2023)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Direct Reprogramming Improves Cardiac Function and Reverses Fibrosis in Chronic Myocardial Infarction

Hidenori Tani et al.

Summary: Adult cardiomyocytes have limited regenerative ability, and the synthesis of extracellular matrix by cardiac fibroblasts (CFs) results in fibrosis and heart dysfunction after myocardial infarction (MI). The overexpression of cardiac transcription factors, MGTH, can directly reprogram CFs into induced cardiomyocytes (iCMs) and improve cardiac function in acute MI. However, it is unclear whether in vivo cardiac reprogramming can repair chronic MI with established scars.

CIRCULATION (2023)

添加到收藏夹

Article Hematology

Single-Cell RNA Sequencing and Assay for Transposase-Accessible Chromatin Using Sequencing Reveals Cellular and Molecular Dynamics of Aortic Aging in Mice

Wenhui Xie et al.

Summary: This study investigated the cellular and molecular mechanisms underlying age-related vascular aging in aortic cellular subpopulations using single-cell RNA sequencing and assay for transposase-accessible chromatin sequencing. The results showed that aortic aging affects cell interactions, inflammation, cell type composition, dysregulation of transcriptional control, and chromatin accessibility. Furthermore, specific endothelial cells and fibroblasts senescence were associated with distinct TF open chromatin states and an mRNA expression model.

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2022)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Delineating chromatin accessibility re-patterning at single cell level during early stage of direct cardiac reprogramming

Haofei Wang et al.

Summary: Direct conversion of cardiac fibroblast into induced cardiomyocytes by cardiac transcription factors shows promise for regenerative medicine. Transcriptome remodelling during cardiac reprogramming is driven by chromatin landscape alteration. The study used single-cell ATAC-seq to identify early chromatin accessibility changes and discovered Smad3 as a bimodal TF in cardiac reprogramming. Integrative analysis with scRNA-seq data revealed active TFs important for iCM conversion and global rewiring of cis-regulatory interactions of cardiac genes along the reprogramming trajectory.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states

Hanbing Song et al.

Summary: The study characterized the microenvironment response to prostate cancer using single-cell RNA-seq and organoids, identifying tumor-associated epithelial cell states and club cells.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Review Cardiac & Cardiovascular Systems

Hooked on heart regeneration: the zebrafish guide to recovery

Katherine M. Ross Stewart et al.

Summary: While humans cannot regenerate their hearts after injury, zebrafish have the ability to fully recover from cardiac insults. The regeneration process in zebrafish involves multiple tissue responses and gene regulatory patterns, with cardiomyocyte regeneration depending on neovascularization and lymphangiogenesis. Studies in zebrafish have provided insights into the mechanisms of cardiovascular repair and regeneration, which can inform strategies for treating human patients with cardiovascular disease.

CARDIOVASCULAR RESEARCH (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Multi-omics single-cell data integration and regulatory inference with graph-linked embedding

Zhi-Jie Cao et al.

Summary: GLUE is a computational framework that bridges the gap between different omics layers by modeling regulatory interactions, and it outperforms state-of-the-art tools in accuracy, robustness, and scalability for heterogeneous single-cell multi-omics data. It has been successfully applied in various challenging tasks.

NATURE BIOTECHNOLOGY (2022)

添加到收藏夹

Article Multidisciplinary Sciences

The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans

Robert C. Jones et al.

Summary: The molecular characterization of cell types using single-cell transcriptome sequencing has led to a revolution in cell biology and provided new insights into the physiology of human organs. In this study, a human reference atlas was created, consisting of nearly 500,000 cells from 24 different tissues and organs, allowing for the molecular characterization of over 400 cell types, their tissue distribution, and tissue-specific variation in gene expression. By using multiple tissues from the same donor, the clonal distribution of T cells, tissue-specific mutation rate in B cells, and the analysis of cell cycle state and proliferative potential of shared cell types across tissues were identified. Additionally, cell type-specific RNA splicing was discovered and analyzed within an individual.

SCIENCE (2022)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

hapln1 Defines an Epicardial Cell Subpopulation Required for Cardiomyocyte Expansion During Heart Morphogenesis and Regeneration

Jisheng Sun et al.

Summary: Research has identified a subset of epicardial cells in zebrafish that play a key role in heart regeneration, marked by expression of hapln1a and hapln1b. These cells are crucial in cardiac growth and regeneration, requiring the function of the hapln1b paralog for normal cardiomyocyte proliferation and muscle regeneration.

CIRCULATION (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Activation of a transient progenitor state in the epicardium is required for zebrafish heart regeneration

Yu Xia et al.

Summary: This study identified a transiently activated epicardial progenitor cell (aEPC) subpopulation marked by ptx3a and col12a1b expression in adult zebrafish hearts. Upon cardiac injury, aEPCs emerged from the epithelial epicardium, underwent epithelial-mesenchymal transition (EMT), and differentiated into different cell types, crucial for heart regeneration.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Spatial multi-omic map of human myocardial infarction

Christoph Kuppe et al.

Summary: In this study, an integrative molecular map of human myocardial infarction was generated using multiple analysis methods. The results elucidated the molecular principles of cardiac tissue organization and provided an important reference for mechanistic and therapeutic studies of cardiac disease.

NATURE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Pathogenic variants damage cell composition and single-cell transcription in cardiomyopathies

Daniel Reichart et al.

SCIENCE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single-cell resolution

Fang Ye et al.

Summary: This study developed a new method of single-cell sequencing to analyze the perturbation patterns of cell type-related gene expression in neotenic and metamorphosed axolotls. By analyzing gene expression of over 1 million single cells, the first adult axolotl cell landscape was constructed. Comparison of single-cell transcriptomes between tissues of neotenic and metamorphosed axolotls revealed the heterogeneity of non-immune parenchymal cells in different tissues and identified their regulatory network.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Review Medicine, General & Internal

Development and validation of a decision support tool for the diagnosis of acute heart failure: systematic review, meta- analysis, and modelling study

Kuan Ken Lee et al.

Summary: This study evaluated the diagnostic performance of N-terminal pro-B-type natriuretic peptide (NT-proBNP) and developed a decision support tool combining NT-proBNP concentrations with clinical characteristics. The results showed substantial variation in guideline recommended thresholds for diagnosing acute heart failure in important patient subgroups. The CoDE-HF decision support tool, incorporating NT-proBNP as a continuous measure and other clinical variables, provides a more consistent, accurate, and individualized approach.

BMJ-BRITISH MEDICAL JOURNAL (2022)

添加到收藏夹

Article Genetics & Heredity

Full-Length Spatial Transcriptomics Reveals the Unexplored Isoform Diversity of the Myocardium Post-MI

Etienne Boileau et al.

Summary: The study introduces a software suite called Single-cell Nanopore Spatial Transcriptomics (scNaST), which facilitates the analysis of spatial gene expression from second- and third-generation sequencing. It allows the generation of a full-length near-single-cell transcriptional landscape of the tissue microenvironment. The researchers demonstrated the workflow using sections of the mouse heart and identified novel modes of transcription associated with the infarct area. These findings have important implications for the development of therapeutic strategies for heart disease.

FRONTIERS IN GENETICS (2022)

添加到收藏夹

Article Hematology

Cellular Landscapes of Nondiseased Human Cardiac Valves From End-Stage Heart Failure-Explanted Heart

Songren Shu et al.

Summary: This study used single-cell RNA sequencing to explore the cellular landscape of nondiseased human cardiac valve leaflets and found differences in cell compositions among different types of valves. The study also identified specific cellular subpopulations associated with the pathogenesis of calcific aortic valve diseases through integrative analysis.

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2022)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

A Human atlas of smooth muscle cell gene expression; insights from the FANTOM consortium CAGE dataset

R Vaja et al.

EUROPEAN HEART JOURNAL (2022)

添加到收藏夹

Review Biochemistry & Molecular Biology

Multimodal biomedical AI

Julian N. Acosta et al.

Summary: This Review discusses the potential applications, technical pitfalls, and challenges of multimodal artificial intelligence models in the field of health and medicine. By utilizing data from large biobanks, electronic health records, medical imaging, and genomics, multimodal AI solutions have the ability to capture the complexity of human health and disease. Key areas such as personalized medicine, digital clinical trials, remote monitoring and care, pandemic surveillance, and virtual health assistants offer opportunities, but data, modeling, and privacy challenges need to be addressed for the full potential of multimodal AI in health to be realized.

NATURE MEDICINE (2022)

添加到收藏夹

Article Oncology

High-resolution single-cell atlas reveals diversity and plasticity of tissue-resident neutrophils in non-small cell lung cancer

Stefan Salcher et al.

Summary: This study investigates the immune cell phenotypes in non-small cell lung cancer (NSCLC) and identifies cellular components associated with tumor histology and genotypes. The study also reveals the plasticity of tissue-resident neutrophils (TRNs) and their association with anti-programmed cell death ligand 1 (PD-L1) treatment failure.

CANCER CELL (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Integrative single-cell analysis of cardiogenesis identifies developmental trajectories and non-coding mutations in congenital heart disease

Mohamed Ameen et al.

Summary: This study defines the multi-cellular epigenomic and transcriptional landscape of cardiac cellular development using single-cell chromatin accessibility maps. The research identifies dynamic transcription factor activity signatures associated with differentiation of primary cardiac cell types, and compares regulatory landscapes of iPSC-derived cardiac cell types with their in vivo counterparts. Additionally, deep learning models are used to interpret chromatin accessibility profiles and identify TF motif lexicons. The study also reveals that de novo mutations affecting chromatin accessibility are enriched in congenital heart disease (CHD) cases and validates their functional impact on developmental cell types in vitro.

CELL (2022)

添加到收藏夹

Article Computer Science, Artificial Intelligence

scBERT as a large-scale pretrained deep language model for cell type annotation of single-cell RNA-seq data

Fan Yang et al.

Summary: Annotating cell types based on single-cell RNA-seq data is crucial for studying disease progress and tumor microenvironments. Existing annotation methods often face challenges such as a lack of curated marker gene lists, difficulties in handling batch effects, and inability to leverage gene-gene interaction information, leading to limited generalization and robustness. In this study, we developed a pretrained deep neural network model called scBERT, which overcomes these challenges by training on large amounts of unlabeled scRNA-seq data and achieving a comprehensive understanding of gene-gene interactions. scBERT demonstrates superior performance in cell type annotation, novel cell type discovery, robustness to batch effects, and model interpretability.

NATURE MACHINE INTELLIGENCE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Single-nucleus profiling of human dilated and hypertrophic cardiomyopathy

Mark Chaffin et al.

Summary: The study identified extensive molecular alterations in failing hearts through single-nucleus RNA sequencing, including changes in transcriptional profiles and the presence of a unique population of activated fibroblasts that are almost absent from non-failing samples. The findings provide new insights into the transcriptional diversity of the human heart in health and disease, as well as potential therapeutic targets and biomarkers for heart failure.

NATURE (2022)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Resolving the intertwining of inflammation and fibrosis in human heart failure at single-cell level

Man Rao et al.

Summary: In this study, single-cell RNA sequencing and single T cell receptor sequencing were used to investigate cellular and molecular alterations in cardiac fibrosis, revealing AEBP1 as a crucial regulator in myofibroblasts. A significant infiltration of leukocytes, especially cytotoxic and exhausted CD8(+) T cells and pro-inflammatory CD4(+) T cells, was observed within fibrotic myocardium. Additionally, a subset of tissue-resident macrophages, CXCL8(hi)CCR2(+)HLA-DRhi macrophage, was identified in severely fibrotic areas, interacting with activated endothelial cells via DARC to potentially facilitate leukocyte recruitment and infiltration in human heart failure.

BASIC RESEARCH IN CARDIOLOGY (2021)

添加到收藏夹

Article Oncology

Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system

Marion Baniol et al.

Summary: This study utilized a transgenic mouse model with the FUCCI system to isolate and characterize cycling cardiomyocytes, as well as perform single-cell transcriptome analysis of cardiomyocytes at different developmental stages. The results showed that cardiomyocytes have the highest mitotic activity at birth, followed by a gradual decline during the neonatal period, with premature cell cycle exit at G1/S shortly after birth and delayed G1/S progression during endoreplication. Single-cell RNA-seq confirmed signaling pathways involved in cardiomyocyte proliferation and maturation-related transcriptional changes during postnatal development. The study generated transcriptional profiles specific to cell division and endoreplication in cardiomyocytes at different developmental stages, which may help identify genes important for adult cardiomyocyte proliferation and heart regeneration.

EXPERIMENTAL CELL RESEARCH (2021)

添加到收藏夹

Editorial Material Biochemical Research Methods

Method of the Year: spatially resolved transcriptomics

Vivien Marx

Summary: Nature Methods has named spatially resolved transcriptomics as Method of the Year 2020.

NATURE METHODS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing

Dandan Liang et al.

Summary: This study identified four distinct transcriptional clusters in the mouse SAN using single-cell RNA sequencing, revealing a core cell cluster enriched in electrogenic genes. Similar cellular features were observed in the SAN from rabbit and cynomolgus monkey. The deficiency of Vsnl1 was found to reduce the beating rate of hiPSC-CMs and the heart rate of mice, suggesting its potential role as a SAN marker. Additionally, a core gene regulation network governing the function of the SAN in mice was unveiled through weighted gene co-expression network analysis (WGCNA).

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas

Xianwen Ren et al.

Summary: The study revealed the presence of immune response dysfunction in COVID-19 patients, with different peripheral immune subtype changes associated with clinical features such as age, sex, severity, and disease stages. Additionally, dramatic transcriptomic changes were observed within virus-infected cells, and upregulation of S100A8/A9 in peripheral blood may contribute to the cytokine storms frequently observed in severe patients.

CELL (2021)

添加到收藏夹

Article Biology

Coronary blood vessels from distinct origins converge to equivalent states during mouse and human development

Ragini Phansalkar et al.

Summary: During heart development, coronary endothelial cells are first influenced by the lineage of progenitor cells, then by their location, until heterogeneity declines in the adult heart. This highlights the plasticity of endothelial cells during development, and the validity of mice as a model for human coronary development.

ELIFE (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Cross-tissue organization of the fibroblast lineage

Matthew B. Buechler et al.

Summary: Recent studies have identified fibroblast heterogeneity in different tissues, and constructed fibroblast atlases based on single-cell transcriptomic data to reveal universal and activated transcriptional subtypes in healthy and diseased states.

NATURE (2021)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Computational principles and challenges in single-cell data integration

Ricard Argelaguet et al.

Summary: The development of single-cell multimodal assays has provided a powerful tool for investigating cellular heterogeneity in multiple dimensions. Data integration is a key challenge in analyzing single-cell multimodal data, with existing strategies utilizing similar mathematical ideas but having distinct goals and principles.

NATURE BIOTECHNOLOGY (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Single-cell multi-omics analysis of the immune response in COVID-19

Emily Stephenson et al.

Summary: Transcriptomic and proteomic profiling of blood samples from individuals with COVID-19 reveals immune cell and hematopoietic progenitor cell alterations that are differentially associated with disease severity.

NATURE MEDICINE (2021)

添加到收藏夹

Article Oncology

Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations

J. Pei et al.

Summary: Through integrating histone acetylome, transcriptome, and proteome profiles, we identified major effector genes and protein networks driving pathological changes in HCM with mutated MYBPC3. Our work identifies 38 highly affected protein-coding genes as potential plasma HCM biomarkers and 9 TFs as potential upstream regulators of these pathomechanisms that may serve as possible therapeutic targets.

CLINICAL EPIGENETICS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Cardiac cell type-specific gene regulatory programs and disease risk association

James D. Hocker et al.

Summary: This study identified cCREs in the human heart and found their associations with cardiac cell types and heart failure. It also discovered that genetic variants associated with cardiovascular diseases are enriched within these cCREs, with some potentially linked to atrial fibrillation.

SCIENCE ADVANCES (2021)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Using machine learning approaches for multi-omics data analysis: A review

Parminder S. Reel et al.

Summary: With the advancement of high-throughput omics technologies, it is crucial for biomedical research to adopt integrative approaches to analyze diverse omics data using machine learning algorithms. This can lead to the discovery of novel biomarkers and improve disease prediction and precision medicine delivery.-Methods in machine learning have enabled researchers to gain a deeper insight into biological systems and provide recommendations for interdisciplinary professionals looking to incorporate machine learning skills in multi-omics studies.

BIOTECHNOLOGY ADVANCES (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Integrated analysis of multimodal single-cell data

Yuhan Hao et al.

Summary: The study introduces a weighted-nearest neighbor analysis framework to learn the relative utility of each data type in each cell, enabling integrative analysis of multiple modalities. Applied to a CITE-seq dataset, the method constructs a multimodal reference atlas of the circulating immune system and successfully identifies and validates previously unreported lymphoid subpopulations.

CELL (2021)

添加到收藏夹

Article Developmental Biology

Prrx1b restricts fibrosis and promotes Nrg1-dependent cardiomyocyte proliferation during zebrafish heart regeneration

Dennis E. M. de Bakker et al.

Summary: The homeobox-containing transcription factor Prrx1b is essential for scar-free regeneration of the zebrafish heart by regulating fibrosis and cardiomyocyte proliferation. Prrx1b is activated in epicardial-derived cells where it inhibits TGF beta ligand expression and collagen production while promoting Nrg1 expression to stimulate cardiomyocyte proliferation, maintaining a balance between fibrosis and regeneration in the injured zebrafish heart.

DEVELOPMENT (2021)

添加到收藏夹

Article Multidisciplinary Sciences

A transcriptional switch governs fibroblast activation in heart disease

Michael Alexanian et al.

Summary: In diseased organs, stress-activated signalling cascades alter chromatin, triggering maladaptive cell state transitions. Inhibition of BET proteins alleviates cardiac dysfunction and reveals a reversible transcriptional switch in fibroblasts. This study identifies MEOX1 as a central regulator of fibroblast activation associated with cardiac dysfunction, providing potential targets for treating fibrotic disease.

NATURE (2021)

添加到收藏夹

Article Cell Biology

Age-dependent transcriptional alterations in cardiac endothelial cells

Uchenna Emechebe et al.

Summary: The study identified age-related transcriptional alterations in cardiac endothelial cells, showing distinct patterns compared to endothelial cells from other organ systems. Gene and pathway enrichment analyses revealed unique responses in cardiac endothelial cells, and single-cell analysis identified specific age-dysregulated genes in aged cardiac endothelial subtypes.

PHYSIOLOGICAL GENOMICS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice

Yanpu Chen et al.

Summary: Research shows that expression of OSKM genes in adult cardiomyocytes can induce these cells to dedifferentiate, conferring regenerative capacity to the heart. This short-term dedifferentiation and reprogramming can ameliorate myocardial damage and facilitate heart regeneration.

SCIENCE (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Coordination of endothelial cell positioning and fate specification by the epicardium

Pearl Quijada et al.

Summary: The study suggests that in the developing heart, cell differentiation and development are influenced by chemokines from epicardium-derived cells, affecting endothelial cell migration and fate determination.

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Quantitative single-cell proteomics as a tool to characterize cellular hierarchies

Erwin M. Schoof et al.

Summary: The study demonstrates a comprehensive experimental and computational workflow that establishes global single-cell mass spectrometry-based proteomics as a tool for large-scale single-cell analyses, enabling the exploration of cellular heterogeneity. The approach presented is capable of consistently quantifying around 1000 proteins per cell within limited instrument time.

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration

Srinivas Allanki et al.

Summary: Il-11/Stat3 signaling in zebrafish has a dual function of limiting fibrosis and promoting tissue regeneration. The research shows that Il-11 signaling can drive cellular reprogramming and antagonize scar formation in adult mammals. This reveals novel targets for regenerative therapies.

SCIENCE ADVANCES (2021)

添加到收藏夹

Article Cell & Tissue Engineering

Fosl1 is vital to heart regeneration upon apex resection in adult Xenopus tropicalis

Hai-Yan Wu et al.

Summary: This study demonstrates the essential role of Fosl1 in cardiomyocyte proliferation and heart regeneration in X. tropicalis, potentially through interaction with JunB to promote expression of cell cycle regulator Ccnt1. Overexpression of Fosl1 enhances cardiomyocyte proliferation, while knockdown leads to suppression of proliferation in both X. tropicalis and neonatal mice. Additionally, Fosl1 knockdown impairs heart regeneration in neonatal mice, while overexpression improves cardiac function in adult mice post-myocardium infarction.

NPJ REGENERATIVE MEDICINE (2021)

添加到收藏夹

Article Biology

Cardiac regeneration in the axolotl is unaffected by alterations in leukocyte numbers induced by lipopolysaccharide and prednisolone

Kathrine Pedersen et al.

Summary: In this study, pro- and anti-inflammatory environments were induced in axolotls during heart regeneration to challenge the normal inflammatory response that facilitates regeneration. Local and systemic leukocyte responses were observed, but cardiac regeneration was found to be structurally and functionally unaffected.

BMC RESEARCH NOTES (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Single-Cell Transcriptome Atlas of Murine Endothelial Cells

Joanna Kalucka et al.

CELL (2020)

添加到收藏夹

Article Cell Biology

Functional Heterogeneity within the Developing Zebrafish Epicardium

Michael Weinberger et al.

DEVELOPMENTAL CELL (2020)

添加到收藏夹

Article Cell Biology

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function

Li Wang et al.

NATURE CELL BIOLOGY (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

Anna S. E. Cuomo et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Transcriptional and Cellular Diversity of the Human Heart

Nathan R. Tucker et al.

CIRCULATION (2020)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Single-Cell Reconstruction of Progression Trajectory Reveals Intervention Principles in Pathological Cardiac Hypertrophy

Zongna Ren et al.

CIRCULATION (2020)

添加到收藏夹

Article Developmental Biology

Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration

Jana Koth et al.

DEVELOPMENT (2020)

添加到收藏夹

Review Cardiac & Cardiovascular Systems

Single-cell RNA sequencing in cardiovascular development, disease and medicine

David T. Paik et al.

NATURE REVIEWS CARDIOLOGY (2020)

添加到收藏夹

Editorial Material Cardiac & Cardiovascular Systems

Microvascular Impairment After Myocardial Infarction It Is Not Just About Obstruction

Jonathan R. Lindner

CIRCULATION-CARDIOVASCULAR IMAGING (2020)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Single-Cell RNA Sequencing Analysis Reveals a Crucial Role for CTHRC1 (Collagen Triple Helix Repeat Containing 1) Cardiac Fibroblasts After Myocardial Infarction

Adrian Ruiz-Villalba et al.

CIRCULATION (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Cells of the adult human heart

Monika Litvinukova et al.

NATURE (2020)

添加到收藏夹

Editorial Material Multidisciplinary Sciences

Single cell transcriptomics comes of age

Sarah Aldridge et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Cell Biology

Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution

Zhaoning Wang et al.

CELL REPORTS (2020)

添加到收藏夹

Article Biotechnology & Applied Microbiology

MOFA plus : a statistical framework for comprehensive integration of multi-modal single-cell data

Ricard Argelaguet et al.

GENOME BIOLOGY (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

Comprehensive Integration of Single-Cell Data

Tim Stuart et al.

CELL (2019)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Single-cell transcriptome analyses reveal novel targets modulating cardiac neovascularization by resident endothelial cells following myocardial infarction

Ziwen Li et al.

EUROPEAN HEART JOURNAL (2019)

添加到收藏夹

Article Biochemical Research Methods

Fast, sensitive and accurate integration of single-cell data with Harmony

Ilya Korsunsky et al.

NATURE METHODS (2019)

添加到收藏夹

Article Biochemistry & Molecular Biology

A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart

Michaela Asp et al.

CELL (2019)

添加到收藏夹

Article Biology

Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart

Hessel Honkoop et al.

ELIFE (2019)

添加到收藏夹

Article Medicine, Research & Experimental

Transcriptions heterogeneity of fibroblasts is a hallmark of the aging heart

Ramon Vidal et al.

JCI INSIGHT (2019)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Early Regenerative Capacity in the Porcine Heart

Lei Ye et al.

CIRCULATION (2018)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart

Juan Tang et al.

CIRCULATION RESEARCH (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

Single-cell profiling of breast cancer T cells reveals a tissue-resident memory subset associated with improved prognosis

Peter Savas et al.

NATURE MEDICINE (2018)

添加到收藏夹

Review Cardiac & Cardiovascular Systems

Therapeutic approaches for cardiac regeneration and repair

Hisayuki Hashimoto et al.

NATURE REVIEWS CARDIOLOGY (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Analysis of cardiomyocyte clonal expansion during mouse heart development and injury

Konstantina-Ioanna Sereti et al.

NATURE COMMUNICATIONS (2018)

添加到收藏夹

Article Genetics & Heredity

Machine Learning on Human Muscle Transcriptomic Data for Biomarker Discovery and Tissue-Specific Drug Target Identification

Polina Mamoshina et al.

FRONTIERS IN GENETICS (2018)

添加到收藏夹

Article Medicine, General & Internal

Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017

Spencer L. G. James et al.

LANCET (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris

Nicholas Schaum et al.

NATURE (2018)

添加到收藏夹

Article Biochemical Research Methods

Deep generative modeling for single-cell transcriptomics

Romain Lopez et al.

NATURE METHODS (2018)

添加到收藏夹

Article Multidisciplinary Sciences

IL-11 is a crucial determinant of cardiovascular fibrosis

Sebastian Schafer et al.

NATURE (2017)

添加到收藏夹

Article Cell Biology

The druggable genome and support for target identification and validation in drug development

Chris Finan et al.

SCIENCE TRANSLATIONAL MEDICINE (2017)

添加到收藏夹

Article Multidisciplinary Sciences

The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies

Chinmoy Patra et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Article Cell & Tissue Engineering

Heart regeneration in the salamander relies on macrophage-mediated control of fibroblast activation and the extracellular landscape

J. W. Godwin et al.

NPJ REGENERATIVE MEDICINE (2017)

添加到收藏夹

Review Cardiac & Cardiovascular Systems

The Biological Basis for Cardiac Repair After Myocardial Infarction From Inflammation to Fibrosis

Sumanth D. Prabhu et al.

CIRCULATION RESEARCH (2016)

添加到收藏夹

Article Physiology

Physiological Implications of Myocardial Scar Structure

William J. Richardson et al.

COMPREHENSIVE PHYSIOLOGY (2015)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Developmental Heterogeneity of Cardiac Fibroblasts Does Not Predict Pathological Proliferation and Activation

Shah R. Ali et al.

CIRCULATION RESEARCH (2014)

添加到收藏夹

Article Biotechnology & Applied Microbiology

DNA methylation age of human tissues and cell types

Steve Horvath

GENOME BIOLOGY (2013)

添加到收藏夹

Article Cell Biology

Complete cardiac regeneration in a mouse model of myocardial infarction

Bernhard Johannes Haubner et al.

AGING-US (2012)

添加到收藏夹

Article Medicine, Research & Experimental

Adult mouse epicardium modulates myocardial injury by secreting paracrine factors

Bin Zhou et al.

JOURNAL OF CLINICAL INVESTIGATION (2011)

添加到收藏夹

Article Multidisciplinary Sciences

Transient Regenerative Potential of the Neonatal Mouse Heart

Enzo R. Porrello et al.

SCIENCE (2011)

添加到收藏夹

Article Biochemical Research Methods

mRNA-Seq whole-transcriptome analysis of a single cell

Fuchou Tang et al.

NATURE METHODS (2009)

添加到收藏夹

Review Genetics & Heredity

RNA-Seq: a revolutionary tool for transcriptomics

Zhong Wang et al.

NATURE REVIEWS GENETICS (2009)

添加到收藏夹

Editorial Material Cardiac & Cardiovascular Systems

Microvascular obstruction and the no-reflow phenomenon after percutaneous coronary intervention

Ronen Jaffe et al.

CIRCULATION (2008)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Coronary microvascular dysfunction after myocardial infarction: increased coronary zero flow pressure both in the infarcted and in the remote myocardium is mainly related to left ventricular filling pressure

P. L. Van Herck et al.

HEART (2007)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse

Indroneal Banerjee et al.

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2007)

添加到收藏夹

Article Biochemistry & Molecular Biology

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors

Kazutoshi Takahashi et al.

CELL (2006)

添加到收藏夹

Article Multidisciplinary Sciences

Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction

H Oh et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

添加到收藏夹

Article Multidisciplinary Sciences

Heart regeneration in zebrafish

KD Poss et al.

SCIENCE (2002)

添加到收藏夹

Article Cardiac & Cardiovascular Systems

Left ventricular remodeling after myocardial infarction - Pathophysiology and therapy

MGS Sutton et al.

CIRCULATION (2000)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.