4.7 Article

Production of isotopically enriched high molecular weight hyaluronic acid and characterization by solid-state NMR

期刊

CARBOHYDRATE POLYMERS
卷 316, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2023.121063

关键词

Hyaluronic acid; Solid-state NMR spectroscopy; Isotopic enrichment; Hydrogels; Mass spectrometry

向作者/读者索取更多资源

Hyaluronic acid (HA), a polysaccharide in the extracellular matrix (ECM) of vertebrate cells, is being widely studied for its applications in hydrogels due to its high viscoelasticity and biocompatibility. However, there are limited techniques available to understand the structural and functional properties of HA-containing hydrogels. In this study, a convenient method to obtain enriched high molecular weight (HMW)-HA labeled with 13C and 15N is presented, which allows for advanced NMR techniques to study the structure and dynamics of HMW-HA-based hydrogels and their interactions with proteins and other ECM components.
Hyaluronic acid (HA) is a naturally occurring polysaccharide that is abundant in the extracellular matrix (ECM) of all vertebrate cells. HA-based hydrogels have attracted great interest for biomedical applications due to their high viscoelasticity and biocompatibility. In both ECM and hydrogel applications, high molecular weight (HMW)-HA can absorb a large amount of water to yield matrices with a high level of structural integrity. To understand the molecular underpinnings of structural and functional properties of HA-containing hydrogels, few techniques are available. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for such studies, e. g. 13C NMR measurements can reveal the structural and dynamical features of (HMW) HA. However, a major obstacle to 13C NMR is the low natural abundance of 13C, necessitating the generation of HMW-HA that is enriched with 13C isotopes. Here we present a convenient method to obtain 13C- and 15N-enriched HMW-HA in good yield from Streptococcus equi subsp. zooepidemicus. The labeled HMW-HA has been characterized by solution and magic angle spinning (MAS) solid-state NMR spectroscopy, as well as other methods. These results will open new ways to study the structure and dynamics of HMW-HA-based hydrogels, and interactions of HMW-HA with proteins and other ECM components, using advanced NMR techniques.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据