Morphophysiological indicators applied for the selection of different genotypes of Hevea spp. during germination and post-germination

We investigate and characterize the morphophysiological changes related to germination and the initial growth and development of seedlings of wild and cultivated genotypes of the genus Hevea spp. in finding to add value to new clones. Seeds from six genotypes (H. brasiliensis (Willd. Ex A. Juss) Mull. Arg, H. guianensis Aubl., H. spruceana (Benth.) Mull. Arg., RRIM 600, Fx 3844, and IAN 873) were sterilized, and their imbibition curve, germination parameters, and initial seedling growth were characterized. In parallel, the morphology of the germination and post-germination stages was monitored from seeds germinated in washed sand. Morphological differences in the seeds/embryos, different strategies in the imbibition process, emergence, and initial seedling growth were observed. The species H. guianensis and H. spruceana showed the greatest differences in size, shape, and color of the seeds/embryos and initial seedling growth. The characteristics of the seed coat patterns, the seedling imbibition, growth, and development processes can serve as morphophysiological markers for genotype identification and selection. This is the first morphophysiological study of seeds and seedlings of wild and cultivated genotypes of species of the genus Hevea. We conclude that there is considerable phenotypic variability among species of the Hevea genus regarding seed and seedling morphophysiology, which can be explored in the rubber tree breeding programs.

Automated summary

We investigated the morphophysiological changes related to germination and initial growth of wild and cultivated genotypes of Hevea spp., aiming to add value to new clones. Sterilized seeds from six genotypes were studied for imbibition curve, germination parameters, and initial seedling growth. Morphological differences in seeds/embryos, imbibition process, emergence, and seedling growth were observed. Seed coat patterns, imbibition, growth, and development processes can be used as markers for genotype identification and selection. This is the first morphophysiological study of Hevea spp. seeds and seedlings. Phenotypic variability in seed and seedling morphophysiology can be explored in rubber tree breeding programs.