Immunoprofiling of cytokines, chemokines, and growth factors in female patients with systemic lupus erythematosus- a pilot study

Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease affecting different organ systems. This study aimed to determine the concentrations of 30 different human cytokines, chemokines, and growth factors in human plasma to understand the role of these markers in the pathogenicity of SLE using Luminex Multiple Analyte Profiling (xMAP) technology. Plasma samples were obtained from patients with SLE (n = 28), osteoarthritis (OA) (n = 9), and healthy individuals (n = 12) were obtained. High levels of TNF, IL-6, IFN-& gamma;, INF-& alpha;, IL-4, IL-5, IL-13, IL-8, IP-10, MIG, MCP-1, MIP-1 & beta;, GM-CSF, G-CSF, EGF, VEGF, IL-12, IL-1RA, and IL-10 was detected in SLE patients compared with the OA and healthy control groups. xMAP analysis has been used to address the differential regulation of clinical heterogeneity and immunological phenotypes in SLE patients. In addition, complete disease phenotyping information along with cytokine immune profiles would be useful for developing personalized treatments for patients with SLE.

Automated summary

This study aimed to understand the role of different cytokines, chemokines, and growth factors in the pathogenicity of SLE by determining their concentrations in human plasma using xMAP technology. Higher levels of various cytokines were detected in SLE patients compared to osteoarthritis patients and healthy individuals. The use of xMAP analysis helps to study the differential regulation of clinical heterogeneity and immunological phenotypes in SLE patients, providing valuable information for personalized treatment development.