期刊
BMC GENOMICS
卷 24, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12864-023-09693-8
关键词
TADs; Chromatin conformation; Epigenetics; Gene expression; Immunoinformatics; Multi-omics; CD4(+) T cells; TCR activation
This study investigates the correlation between TAD rearrangements and gene expression changes in TCR-induced CD4(+) T cells using Hi-C and RNA-seq data. The findings suggest a significant relationship between TAD rearrangements and changes in local gene expression, and provide new insights into the global mechanisms that regulate gene expression.
Background Gene expression has long been known to be influenced by the relative proximity of DNA regulatory elements. Topologically associating domains (TADs) are self-interacting genomic regions involved in regulating gene expression by controlling the proximity of these elements. Prior studies of TADs and their biological roles have revealed correlations between TAD changes and cellular differentiation. Here, we used Hi-C and RNA-seq data to correlate TCR-induced changes in TAD structure and gene expression in human CD4(+) T cells. Results We developed a pipeline, Differentially Expressed Gene Enrichment Finder (DEGEF), that identifies regions of differentially expressed gene enrichment. Using DEGEF, we found that TCR-regulated genes cluster non-uniformly across the genome and that these clusters preferentially localized in regions of TAD rearrangement. Interestingly, clusters of upregulated genes preferentially formed new Hi-C contacts compared to downregulated clusters, suggesting that TCR-activated CD4(+) T cells may regulate genes by changing stimulatory contacts rather than inhibitory contacts. Conclusions Our observations support a significant relationship between TAD rearrangements and changes in local gene expression. These findings indicate potentially important roles for TAD rearrangements in shaping their local regulatory environments and thus driving differential expression of nearby genes during CD4(+) T cell activation. Moreover, they provide new insights into global mechanisms that regulate gene expression.
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