Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris

Review Food Science & Technology

Decontamination of Aflatoxins in Edible Oils: A Comprehensive Review

Fardin Javanmardi et al.

Summary: The contamination of aflatoxins in crops can lead to health problems in edible oil. This review focuses on different methods to degrade or reduce aflatoxins in edible oils, including chemical, physical, and biological methods. Chemical and physical techniques such as UV irradiation, gamma irradiation, pulsed light, and absorbents have been proposed, but there is a lack of research on biological methods.

FOOD REVIEWS INTERNATIONAL (2022)

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Article Fisheries

Evaluation of corn distillers dried grains with solubles (DDGS) replacement for fishmeal in the diet for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ x Epinephelus lanceolatus ♂ )

Zhanhao Zhu et al.

Summary: This study determined the optimal level of corn distillers dried grains with solubles (DDGS) substitution in the diet of juvenile hybrid grouper. The results showed that a substitution level of 7.80% significantly improved the growth performance and intestinal health of the fish.

AQUACULTURE REPORTS (2022)

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Article Biology

Optimization and Validation of ELISA for Aflatoxin B1 Detection in Fermented Forages and Feeds

Eniko Horvath et al.

Summary: Enzyme-coupled immunosorbent assays (ELISA) methods are commonly used for mycotoxin measurement in homogeneous matrixes like corn and wheat. However, there is a lack of research on complex materials such as fermented forages and mixed feed. This study optimized the sample preparation and validated the accuracy and repeatability of the analysis for aflatoxin B1 in fermented forages and mixed feed.

SCIENTIFICA (2022)

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Article Food Science & Technology

Aflatoxins in maize dough and dietary exposure in rural populations of Togo

M. D. Hanvi et al.

Summary: Aflatoxins produced by fungi pose food safety challenges in subSaharan African countries, particularly in maize-based products like maize dough. The study in Togo found high levels of aflatoxins in maize dough, with children being the most exposed group. The excess risk of liver cancer associated with aflatoxin consumption in the southern rural population of Togo was discussed.

FOOD CONTROL (2021)

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Article Food Science & Technology

Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts

Peizhou Yang et al.

Summary: The study integrated the Trametes versicolor aflatoxin B-1-degrading enzyme gene into the genome of Pichia pastoris and demonstrated its effective degradation of AFB(1). The recombinant enzyme showed high activity in degrading AFB(1), indicating its potential application in food crops.

TOXINS (2021)

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Article Biotechnology & Applied Microbiology

Activity enhancement of Trametes versicolor aflatoxin B1-degrading enzyme (TV-AFB1D) by molecular docking and site-directed mutagenesis

Peizhou Yang et al.

Summary: In this study, the catalytic performance of TV-AFB(1)D was improved through molecular docking and site-directed mutagenesis techniques. Among the mutants, E436A/H554A showed the highest enzyme activity, indicating a potential for enhancing AFB(1) degradation.

FOOD AND BIOPRODUCTS PROCESSING (2021)

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Review Food Science & Technology