期刊
BIOTECHNOLOGY ADVANCES
卷 66, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2023.108171
关键词
Enzyme; Biochemical characterization; Biotechnology; Catalytic activity; Thermostability; Steady-state kinetics; Protein crystallography; Big data; Protein engineering; Artificial intelligence
Nowadays, there is a growing demand for novel biotechnological products, and biocatalytic applications are playing a vital role in providing sustainable alternatives to chemical processes. However, the characterization of enzyme variants for industrial processes remains a limiting factor. While there are a few microfluidic systems available for enzyme analysis, the transformation of prototypes into commercial platforms needs to be streamlined. This review discusses the state-of-the-art microfluidic tools for the analysis of biocatalysts, their advantages, disadvantages, and potential for leveraging machine learning.
Nowadays, the vastly increasing demand for novel biotechnological products is supported by the continuous development of biocatalytic applications that provide sustainable green alternatives to chemical processes. The success of a biocatalytic application is critically dependent on how quickly we can identify and characterize enzyme variants fitting the conditions of industrial processes. While miniaturization and parallelization have dramatically increased the throughput of next-generation sequencing systems, the subsequent characterization of the obtained candidates is still a limiting process in identifying the desired biocatalysts. Only a few commercial microfluidic systems for enzyme analysis are currently available, and the transformation of numerous published prototypes into commercial platforms is still to be streamlined. This review presents the state-of-the-art, recent trends, and perspectives in applying microfluidic tools in the functional and structural analysis of biocatalysts. We discuss the advantages and disadvantages of available technologies, their reproducibility and robustness, and readiness for routine laboratory use. We also highlight the unexplored potential of microfluidics to leverage the power of machine learning for biocatalyst development.
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