Electrochemical fluorescence switching of enhanced green fluorescent protein

Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different wavelengths. Herein, we report an electrochemical approach to reversible fluorescence switching for enhanced green fluorescent proteins (EGFP) on indium tin oxide coated glass. Our results demonstrate that negative and positive electrochemical potentials can efficiently switch the fluorescent proteins between the dim (OFF) and bright (ON) states at the single molecule level. The electrochemical fluorescence switching is fast, reversible, and may be performed up to hundreds of cycles before photobleaching occurs. These findings highlight that this method of electrochemical fluorescence switching can be incorporated into advanced fluorescence microscopy.

Automated summary

This study presents an electrochemical approach to reversible fluorescence switching of enhanced green fluorescent proteins (EGFP) on indium tin oxide coated glass. The method allows efficient switching between bright (ON) and dim (OFF) states at the single molecule level. The electrochemical fluorescence switching is fast, reversible, and can be incorporated into advanced fluorescence microscopy.