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Biotechnology & Applied Microbiology
Xinyu Yu et al.
Summary: This study developed a CRISPRi-microfluidics screening platform to identify crucial gene targets for enhancing r-protein secretion in Corynebacterium glutamicum. By screening 46,549 single-guide RNAs, unknown targets related to transmembrane transport, amino-acid metabolism, and redox regulation were discovered. These findings were used to construct a hyperproducer for multiple proteins through combinatorial engineering of redox-response transcription factors. The CRISPRi technology and FlAsH-based screening platform used in this study have broad applications for other microbial species and recombinant proteins.
METABOLIC ENGINEERING
(2023)
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Biotechnology & Applied Microbiology
Rebecca M. Lennen et al.
Summary: This study used an automated platform to evolve Escherichia coli to grow optimally in the presence of 11 industrial chemicals and uncovered the tolerance mechanisms. The results showed that cells are tolerized through frequent mutation of membrane transporters, cell wall-associated proteins, transcription and translation machineries, and stress signaling proteins. Osmotic stress plays a significant role in tolerance, and pre-tolerized starting isolates can greatly enhance the subsequent production of chemicals.
METABOLIC ENGINEERING
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Shuai Jiang et al.
Summary: This study combined multilevel rational metabolic engineering with biosensor-assisted mutagenesis screening to exploit the L-arginine production potential of Escherichia coli. Multiple metabolic pathways were reprogrammed to redirect the metabolic flux into L-arginine synthesis, and a toggle switch was designed to dynamically control gene expression. A biosensor-assisted high-throughput screening platform was used to further explore L-arginine production potential. Through this approach, the engineered strain produced the highest values of L-arginine reported so far. The study demonstrated the effectiveness of rational metabolic reprogramming and biosensor-assisted mutagenesis screening in unleashing cellular potential for value-added metabolite production.
METABOLIC ENGINEERING
(2023)
Review
Biochemical Research Methods
Tingting Guo et al.
Summary: Predicting phenotype with genomic and environmental information using machine learning methods is challenging but necessary. This review discusses the progress of phenotype prediction models enabled or improved by machine learning. The applications are categorized into scenarios based on genotypic information, environmental information, and both, highlighting the practicality, advantages, and challenges of modeling complex relationships. The potential of leveraging machine learning and genetics theories to develop models that predict phenotype and interpret biological consequences is also discussed.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qun Liu et al.
Summary: A methyl supply system driven by betaine was developed to increase the availability of S-adenosyl-L-methionine (SAM) and improve the production of methylated metabolites. The system improved the titer and productivity of ferulic acid by 12.6-fold and 15.9-fold, respectively, and was successfully applied to other methyltransferase-catalyzed reactions.
METABOLIC ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Jiao Liu et al.
Summary: In this study, the authors use CRISPR technology and CRISPRi screening to convert a wild-type Corynebacterium glutamicum to a hyperproducer of L-proline, an amino acid with various applications. By optimizing the biosynthetic enzyme and regulating the carbon flux, they achieved efficient production of L-proline.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yameng Xu et al.
Summary: This study reports the engineering of Saccharomyces cerevisiae to produce rubusoside and rebaudiosides, which are considered as the next generation of sugar substitutes. Through a modular engineering approach, the authors were able to overcome several limitations and achieve high titers of these molecules. This research provides a sustainable and scalable solution for the production of diverse rebaudiosides.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Rajnigandha Pushpker et al.
Article
Agricultural Engineering
Zhenqiang Zhao et al.
Summary: L-arginine is an important amino acid used in food and pharmaceutical industries. This study investigated the metabolic mechanisms and fermentation conditions affecting L-arginine production. By generating mutant strains and conducting multi-omic analysis, vital mutations in the L-arginine operon and glutamate synthase were identified. The study also applied systematic metabolic engineering to enhance L-arginine production by modifying key enzymes and increasing cofactor availability. Through fermentation optimization, the engineered strain produced a high yield of L-arginine. This study provides new insights into boosting L-arginine production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Liyan Zhu et al.
Summary: In this study, a novel microalgae strain Acutodesmus sp. HLGY was isolated and identified. The feasibility of using melatonin (MT) to increase lutein yield of this strain was evaluated. Results showed that under the 7.5 μM MT treatment, the lutein content and lutein productivity were significantly increased, along with the upregulation of key lutein synthesis-and antioxidant enzyme-related genes. Moreover, MT treatment suppressed carbohydrate, protein, ROS levels, and lipid content, while promoting ethylene and gamma-aminobutyric acid contents.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mohammad Rifqi Ghiffary et al.
Summary: This study constructed a Corynebacterium glutamicum strain through systems metabolic engineering for the highly efficient production of 13-alanine. Various metabolic pathways were optimized and engineered in the strain, resulting in a high yield and productivity of 13-alanine. The findings provide an important contribution to the sustainable and efficient production of 13-alanine as an alternative to chemical synthesis.
METABOLIC ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Jae Sung Cho et al.
Summary: The sustainable production of chemicals from renewable, nonedible biomass is an important alternative to address environmental issues, and microbial cell factories can be engineered to achieve this goal. This article discusses recent trends in designing different types of biosynthetic pathways and explores the development and challenges of systems metabolic engineering.
Article
Biotechnology & Applied Microbiology
Bo Xiong et al.
Summary: The study demonstrates the successful enhancement of L-tryptophan production in Escherichia coli by redistributing central carbon metabolism to improve PEP and E4P pools. Through a combination of metabolic engineering strategies, the engineered strain achieved a high overall yield of L-tryptophan in batch fermentation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Agricultural Engineering
Liyuan Hou et al.
Summary: This study investigated the bioconversion of acid whey to biodegradable polyhydroxybutyrate (PHB) using recombinant E. coli LSBJ, achieving high PHB yields. Factors such as lactate:lactose ratios and C/N impacted metabolic flux and PHB yields, while maintaining fermentation pH and adding inorganic nitrogen sources, minerals, and trace metals promoted PHB production. Ultimately, the study improved understanding of AW utilization factors and demonstrated the potential for sustainable PHB production using recombinant E. coli.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Hyun Jeong Lee et al.
Summary: This study developed a biocontainment system using genetically modified cyanobacteria to produce alpha-farnesene, by deleting gene clusters related to the beta-carboxysome and the carbon concentrating mechanism. The production of alpha-farnesene was dependent on CO2 concentration in the photobioreactor, with potential applications in next generation bio-solar cell factories.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Shuai Jiang et al.
Summary: This study successfully constructed a microbial cell factory for high-level L-citrulline production, achieving the highest reported values through a combination of rational module engineering strategies and dynamic regulation strategies.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Chang Dong et al.
Summary: This study identified novel targets for enhancing SAM production through genome-scale engineering (GSE) and validated their effects in industrial yeast strains, leading to significant increases in SAM productivity. Transcriptomic and metabolomic analyses were conducted to investigate the molecular mechanisms for enhanced SAM accumulation.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Shuaiwen Wang et al.
Summary: In this study, the impact of ProP, ProVWX, and betABIT on l-threonine production in E. coli was investigated. Deletion of proP or/and proVWX significantly increased l-threonine production, while deletion of betABIT slightly decreased l-threonine production. Deleting either crr or ptsG from the resulting strain further improved l-threonine production, with a productivity increase of 116% compared to the control strain TWF001.
MICROBIAL CELL FACTORIES
(2021)
Article
Multidisciplinary Sciences
Lixia Fang et al.
Summary: The study successfully identified gene targets for high-titer production of free fatty acids in E. coli using CRISPRi and omics analyses. By combinatorial perturbation of beneficial genes, a recombinant strain was engineered to produce 30.0 g L-1 FFAs, the highest reported titer in E. coli to date, demonstrating the potential for rewiring cellular metabolism to facilitate biochemical production.
NATURE COMMUNICATIONS
(2021)
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Heyun Wu et al.
ACS SYNTHETIC BIOLOGY
(2020)
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Kento Tokuyama et al.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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Yanan Hao et al.
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Heyun Wu et al.
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(2018)
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Huibin Zou et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
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Yifan Li et al.
METABOLIC ENGINEERING
(2015)
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Agricultural Engineering
Li-Bo Yang et al.
BIORESOURCE TECHNOLOGY
(2014)
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Jianzhong Xu et al.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2014)
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Biotechnology & Applied Microbiology
Kanchana R. Kildegaard et al.
METABOLIC ENGINEERING
(2014)
Article
Biochemical Research Methods
Margarida Carvalho et al.
Review
Chemistry, Multidisciplinary
William N. Lipscomb et al.
ACCOUNTS OF CHEMICAL RESEARCH
(2012)
Review
Biochemical Research Methods
Judith Becker et al.
CURRENT OPINION IN BIOTECHNOLOGY
(2012)
