期刊
BIORESOURCE TECHNOLOGY
卷 391, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2023.129963
关键词
L-Histidine; Marine Biomass; Galactose Utilization; L-Histidine Synthetic Pathway; Metabolic Engineering
This study presents a sustainable approach to L-histidine synthesis by using D-galactose as a carbon source obtained from red algae. The production of L-histidine was enhanced by modifying precursor pathways in the bacteria. Additionally, a galactose utilization system was introduced to efficiently convert D-galactose into L-histidine.
L-Histidine plays significant roles in the food and pharmaceutical industries, and its demand has been steadily increasing recently. As demand for L-histidine continues, the development of eco-friendly processes is required. To pursue this goal, D-galactose, a primary component of red algae, was employed as a carbon source for synthesizing L-histidine. To harness this marine biomass, kappa-carrageenan was preferentially hydrolyzed to obtain Dgalactose using kappa-carrageenase (CgkA) and iduronate-2-sulfatase (IdsA3). Subsequently, L-histidine production was enhanced by modifying precursor pathways in Corynebacterium glutamicum. The resulting strain, TDPH6 exhibited a remarkable 2.15-fold increase in L-histidine production compared to TDP. Furthermore, a galactose utilization system was introduced and named TDPH6G2. During fermentation, this strain efficiently consumed 100 % of the D-galactose and synthesized 0.395 g/L of L-histidine. In conclusion, this study presents a sustainable approach to L-histidine synthesis by introducing a galactose utilization system into C. glutamicum.
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