4.8 Review

Production of polyhydroxyalkanoates as a feasible alternative for an integrated multiproduct lignocellulosic biorefinery

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Article Energy & Fuels

Lignin to polyhydroxyalkanoate bioprocessing by novel strain of Pseudomonas monteilii

Pornkamol Unrean et al.

Summary: This study optimized a fed-batch process using lignin-rich black liquor as carbon source for the production of polyhydroxyalkanoate (PHA). The optimized feeding profiles and C:N ratio significantly enhanced the lignin conversion efficiency and PHA synthesis.

BIOMASS CONVERSION AND BIOREFINERY (2023)

Article Biochemical Research Methods

Microbial conversion of lignin rich biomass hydrolysates to medium chain length polyhydroxyalkanoates (mcl-PHA) using Pseudomonas putida KT2440

Suveera Bellary et al.

Summary: The present study investigates the production capabilities of medium chain length polyhydroxyalkanoates (mcl-PHA) by Pseudomonas putida KT2440 from a mixture of compounds produced from lignocellulosic biomass deconstruction. The results show that Pseudomonas putida KT2440 is capable of producing high-quality PHA on lignocellulosic biomass hydrolysates.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2023)

Article Agricultural Engineering

Consolidated bioprocessing of plant biomass to polyhydroxyalkanoate by co-culture of Streptomyces sp. SirexAA-E and Priestia megaterium

Vijay Kumar et al.

Summary: This study demonstrated the consolidated bioconversion of plant biomass to polyhydroxyalkanoate (PHA) by co-culturing two specialized bacteria, Streptomyces sp. SirexAA-E and Priestia megaterium. The co-culture produced poly(3-hydroxybutyrate) (PHB) using purified polysaccharides and various plant biomass as carbon sources. The optimized co-culture ratio of S. sp. SirexAA-E to P. megaterium produced 40 mg PHB/g Miscanthus using 0.5% biomass loading. This study provides a proof-of-concept for one-pot bioconversion of plant biomass into PHB without separate saccharification processes.

BIORESOURCE TECHNOLOGY (2023)

Article Chemistry, Multidisciplinary

Development of an Integrated Biorefinery System for Bioconversion of Lignocellulosic Biomass to Polyhydroxyalkanoates and Biohydrogen

S. Kavitha et al.

Summary: In this study, a phase-separated pretreatment technique was used to fractionate lignin and cellulose-rich residue from rice straw biomass for polyhydroxyalkanoate (PHA) and biohydrogen production. The results showed that the combined H2O2-homogenizer pretreatment achieved a higher lignin removal rate. The cellulose-rich residue after pretreatment had higher chemical oxygen demand solubilization and cellulose solubilization rates, as well as higher PHA production. Economic analysis showed that the phase-separated pretreatment was more economically feasible.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2023)

Review Environmental Sciences

Polyhydroxyalkanoates (PHAs) synthesis and degradation by microbes and applications towards a circular economy

Wen Zhou et al.

Summary: Overusing non-degradable plastics leads to environmental issues, advocating the use of biodegradable plastics. Polyhydroxyalkanoates (PHAs) are promising biodegradable plastics that can be produced by various microbes using waste feedstock. However, the higher cost of PHAs production compared to fossil-based plastics hinders further industrial production and applications.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2023)

Article Agricultural Engineering

An insight into omics analysis and metabolic pathway engineering of lignin-degrading enzymes for enhanced lignin valorization

Manish Paul et al.

Summary: This review focuses on the research progress of lignin-degrading microorganisms and enzymes associated with ligninolytic activity, which play a crucial role in the breakdown of lignocellulosic biomass. Analyzing multi-omics data highlights the potential value of fungal-produced enzymes in lignocellulosic material degradation, giving them an advantage in their ecological niches.

BIORESOURCE TECHNOLOGY (2023)

Review Environmental Sciences

Current status of microplastics pollution in the aquatic environment, interaction with other pollutants, and effects on aquatic organisms

Tian Zhang et al.

Summary: This paper summarizes the abundance and types of microplastics in different aqueous environments, and discusses their interactions and toxicity with other contaminants. The combined toxic effects of microplastics and other pollutants are highlighted, and future research focuses on the characterization of microplastics, standardization of units, exploring interactions and toxicity, and degradation for a better understanding of ecological risks.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022)

Review Biotechnology & Applied Microbiology

From waste to wealth: upcycling of plastic and lignocellulosic wastes to PHAs

Nedal Y. Abu-Thabit et al.

Summary: In the past fifty years, environmental pollution caused by non-biodegradable petroleum-based plastics has become a serious threat to marine life and human health. Solutions to plastic pollution are urgently needed to reduce soil and water contamination. Developing alternative biodegradable plastics derived from renewable resources is an emerging research focus that can alleviate the accumulation of plastic waste in the environment.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2022)

Article Green & Sustainable Science & Technology

Recent developments in lignocellulosic biomass pretreatment with a focus on eco-friendly, non-conventional methods

Arushdeep Sidana et al.

Summary: This review discusses the modes of action, advantages and limitations of selective non-conventional pretreatment methods for lignocellulosic biomass. Ultrasound and microwave pretreatment have been extensively studied and shown promising results. However, superficial cost and energy analyses are insufficient to support their transition to industrial scale.

JOURNAL OF CLEANER PRODUCTION (2022)

Article Energy & Fuels

Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues

A. Valdez-Calderon et al.

Summary: This study successfully recovered four bacterial strains capable of producing polyhydroxybutyrate (PHB), with one strain identified as having high production potential. The use of low-cost media, such as fruit peel residues, showed promising results for the growth and PHA production of these bacterial strains.

BIOMASS CONVERSION AND BIOREFINERY (2022)

Article Polymer Science

Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass

Rijuta Ganesh Saratale et al.

Summary: This study examines the production of polyhydroxyalkanoates (PHA) by individual microbial cultures and co-cultures using sugarcane bagasse hydrolysates. The co-culture strategy was found to be more effective in sugar assimilation, bacterial growth, and PHA production compared to individual strains. The addition of cost-effective nutrient supplements and volatile fatty acids further enhanced cell growth and PHA production.

POLYMERS (2022)

Review Gastroenterology & Hepatology

Cellulolytic bacteria in the large intestine of mammals

Alicia Froidurot et al.

Summary: The utilization of dietary cellulose by cellulolytic bacteria in the large intestine of mammals plays a crucial role in host nutrition and health. This review retraces the history of cellulolytic bacteria discovery, discusses the methods for isolating these bacteria, summarizes the enzymes and mechanisms involved in cellulose degradation, and presents the contribution of these bacteria to the host.

GUT MICROBES (2022)

Article Environmental Sciences

Pure cultures for synthetic culture development: Next level municipal waste treatment for polyhydroxyalkanoates production

Kasra Khatami et al.

Summary: The study found that VFAs derived from food waste and primary sludge are cheap carbon sources for maintaining high PHA production yields in bacterial cultures. PHA production was higher in mono-cultures compared to co-cultures, with a higher percentage of 3-hydroxyvalerate in the biopolymers.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2022)

Article Green & Sustainable Science & Technology

Lignocellulosic biomass as renewable feedstock for biodegradable and recyclable plastics production: A sustainable approach

Tirath Raj et al.

Summary: The rapid increase in disposal of fossil-derived single-use plastics has led to the accumulation of plastic waste and ecological imbalance. Developing alternative environmentally friendly, biodegradable, and biobased plastics using lignocellulosic biomass offers a sustainable solution. This review explores the potential of lignocellulosic biomass to produce biodegradable and recyclable plastics, highlighting pretreatment methods and challenges in the integrated biorefinery approach. The use of energy chemicals produced through biorefinery processes may serve as a precursor for sustainable bioplastics production. Various recyclable and biodegradable polymers are critically reviewed, and the limitations and future prospects of commercially available biorefinery industries are discussed.

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Biopolymer: A Sustainable Material for Food and Medical Applications

Jaya Baranwal et al.

Summary: Biopolymers are highly valuable functional materials that attract researchers and professionals from various disciplines. Interdisciplinary research is crucial to explore the fundamental and applied aspects of biopolymers in addressing complex problems related to health and well-being. Efforts have been made to replace synthetic polymers with biodegradable materials derived from natural resources to reduce environmental impact. This review focuses on the diverse applications of biopolymers in the food and medical industry and provides an outlook for the future of this industry.

POLYMERS (2022)

Article Environmental Sciences

Hydrodynamic cavitation pre-treatment of urban waste: Integration with acidogenic fermentation, PHAs synthesis and anaerobic digestion processes

A. Lanfranchi et al.

Summary: This paper explores the effect of hydrodynamic cavitation (HC) pre-treatment on the acidogenic co-fermentation process of sewage sludge and vegetable waste, finding that a 50-minute cavitation treatment resulted in the highest increase in sCOD and VFAs. The organic acid-rich broth generated was then evaluated as a substrate for polyhydroxybutyrate storage, yielding a biopolymer with similar characteristics to commercial equivalents. The valorization of the solid-rich fraction of the fermented effluent was also assessed.

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Valorization of Lignocellulose by Producing Polyhydroxyalkanoates under Circular Bioeconomy Premises: Facts and Challenges

Vaibhav Vilas Andhalkar et al.

Summary: Given the current concerns about environmental issues, biodegradable plastics as eco-friendly products have gained attention. Polyhydroxyalkanoates (PHAs) as microbial polyesters contribute to a more sustainable commercial plastic life cycle through a circular bioeconomy. However, the large-scale and sustainable production of PHAs remains challenging, requiring technological solutions and improved production efficiency.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Corn stover hydrolysate and levulinic acid: Mixed substrates for short-chain polyhydroxyalkanoate production

Richard D. Ashby et al.

Summary: In this study, biodegradable plastic substitutes, poly(hydroxyalkanoate) (PHA) biopolymers, were synthesized from corn stover and levulinic acid (LevA). Two types of bacteria, Azohydromonas lata and Burkholderia sacchari, were used to produce terpolyesters and copolymers of PHA. The results showed that detoxification treatment was necessary for A. lata, while B. sacchari could use the hydrolysate without detoxification. This study could help reduce the cost and increase the applicability of bacterially derived PHA biopolymers.

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY (2022)

Article Agricultural Engineering

Biotransformation of toxic lignin and aromatic compounds of lignocellulosic feedstock into eco-friendly biopolymers by Pseudomonas putida KT2440

Saddam Hussain Mohammad et al.

Summary: This study successfully utilized Pseudomonas putida KT2440 to valorize lignin and its derivatives, and synthesized bio-polymers without any genetic manipulation.

BIORESOURCE TECHNOLOGY (2022)

Review Agricultural Engineering

Valorization of lignocellulosic biomass for polyhydroxyalkanoate production: Status and perspectives

Yu Jung Sohn et al.

Summary: This review provides an overview of the production of biodegradable polymers from lignocellulosic biomass (LCB). By establishing a LCB-based biorefinery with proper pretreatment techniques, various polyhydroxyalkanoates (PHA) can be produced from LCB-derived sugars, hydrolysates, and/or aromatic mixtures using microorganisms, offering a solution to the current environmental crises.

BIORESOURCE TECHNOLOGY (2022)

Article Agricultural Engineering

Upcycling agricultural waste to biodegradable polyhydroxyalkanoates by combined ambient alkaline pretreatment and bacterial fermentation

Md Anwar Hossain et al.

Summary: Researchers have developed an environmentally friendly method for producing mcl-PHAs from rice straw and hemp hurd. The method involves alkaline pretreatment, enzymatic hydrolysis, and bacterial fermentation, resulting in high sugar yield and PHAs production. This energy-efficient process can be applied to other sources of lignocellulose.

INDUSTRIAL CROPS AND PRODUCTS (2022)

Article Energy & Fuels

Unraveling PHA production from urban organic waste with purple phototrophic bacteria via organic overload

Luis D. Allegue et al.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2022)

Article Biotechnology & Applied Microbiology

Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass

Ruolin Qin et al.

Summary: In recent years, microbial consortia composed of engineered Pseudomonas putida and Escherichia coli have become the focus of research for their high polymer yield and synthesis capacity in mcl-PHA production.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

Review Agricultural Engineering

Production and recovery of polyhydroxyalkanoates (PHA) from waste streams-A review

R. Yukesh Kannah et al.

Summary: Polyhydroxyalkanoates (PHA) are a more attractive sustainable green plastic with the potential to replace petroleum-based plastics. Producing green plastic from various waste streams is environmentally friendly and cost-effective.

BIORESOURCE TECHNOLOGY (2022)

Article Biotechnology & Applied Microbiology

From Agri-Food Wastes to Polyhydroxyalkanoates through a Sustainable Process

Federico Verdini et al.

Summary: This study utilized corn straw as raw material to obtain a mixture of fermentable sugars through microwave-assisted flash hydrolysis. Scale-up in a stirred tank bioreactor resulted in higher yields and concentration. The possibility of producing PHAs from agricultural waste using a mixed microbial culture from the food industry with enabling technologies could make the production of biopolymers more competitive.

FERMENTATION-BASEL (2022)

Article Green & Sustainable Science & Technology

Emerging approaches in lignocellulosic biomass pretreatment and anaerobic bioprocesses for sustainable biofuels production

Ramesh Kumar et al.

Summary: Various eco-friendly approaches are crucial for efficient conversion of biomass to ensure high yields of biofuels as a commercial solution. Utilizing a range of microbes and enzymes can effectively convert lignocellulosic biomass into a biodegradable structure, facilitating its subsequent valorization.

JOURNAL OF CLEANER PRODUCTION (2022)

Review Biotechnology & Applied Microbiology

A New Wave of Industrialization of PHA Biopolyesters

Martin Koller et al.

Summary: The increasing use of plastics and their negative impact on the environment has led to the development of renewable and biodegradable materials. Polyhydroxyalkanoates (PHAs), a class of biopolymers synthesized by microorganisms, have properties similar to petrochemical plastics but can biodegrade in various environments. The emergence of the PHA industry has attracted the attention of chemical companies, start-ups, and brand owners who are now producing and utilizing PHAs in various applications. This commercialization wave of PHAs holds great potential in reducing plastic pollution and fighting climate change.

BIOENGINEERING-BASEL (2022)

Article Engineering, Environmental

Polyhydroxyalkanoate (PHA) Production Using Volatile Fatty Acids Derived from the Anaerobic Digestion of Waste Paper

Huda Al Battashi et al.

Summary: The study demonstrated that utilizing waste paper as a resource for PHA production through anaerobic digestion is a low-cost strategy. By optimizing the synthesis conditions of PHA from VFAs produced from waste paper, a significantly higher yield of PHA was achieved with a limited nutrient medium.

JOURNAL OF POLYMERS AND THE ENVIRONMENT (2021)

Article Agricultural Engineering

Efficient poly(3-hydroxybutyrate-co-lactate) production from corn stover hydrolysate by metabolically engineered Escherichia coli

Ju Wu et al.

Summary: This study investigated the effect of corn stover hydrolysate (CSH) as a carbon source on the biosynthesis of P(3HB-co-LA), finding that reducing the activity of the electron transport system led to a higher fraction of lactate (LA) in the copolymer, and replacing the promoter of the key gene pctth with ldhA gene promoter dynamically modulated the expression of pctth gene and changed the lactic acid content.

BIORESOURCE TECHNOLOGY (2021)

Article Chemistry, Physical

Manufacturing energy and greenhouse gas emissions associated with plastics consumption

Scott R. Nicholson et al.

Summary: Comparing the supply chain energy requirements and greenhouse gas emissions of bio-based and/or circular plastic production to incumbent manufacturing paradigms is essential for informing the development of future disruptive technologies in the synthetic polymer industry.
Article Agricultural Engineering

Valorizing waste liquor from dilute acid pretreatment of lignocellulosic biomass by Bacillus megaterium B-10

Jiawei Li et al.

Summary: An efficient biostrategy was developed to upgrade waste sugar in Dilute Acid Pretreatment Liquor (DAPL) of lignocellulose, using Bacillus megaterium B-10 to convert the waste sugar into high-valuable polyhydroxyalkanoate (PHA). The concentration of sugar and phenolics in DAPL can reach certain levels after pretreating rice straw, and a demonstrated yield of poly-3-hydroxybutyrate (PHB) was achieved through this bioconversion.

INDUSTRIAL CROPS AND PRODUCTS (2021)

Article Agricultural Engineering

Polyhydroxybutyrate production from ultrasound-aided alkaline pretreated finger millet straw using Bacillus megaterium strain CAM12

Sivagnanam Silambarasan et al.

Summary: In this study, finger millet straw (FMS) was utilized for the production of Polyhydroxybutyrate (PHB) by Bacillus megaterium strain CAM12. The combination of ultrasound-assisted alkaline pretreatment and enzymatic saccharification of FMS resulted in high delignification and hydrolysis yields, leading to successful PHB production by the strain CAM12. The extracted polymer was confirmed to be PHB through analytical techniques like FTIR, XRD, and NMR, highlighting the potential of FMS hydrolysates as a promising feedstock for PHB production.

BIORESOURCE TECHNOLOGY (2021)

Article Biochemistry & Molecular Biology

Utilization of Agave durangensis leaves by Bacillus cereus 4N for polyhydroxybutyrate (PHB) biosynthesis

Raul E. Martinez-Herrera et al.

Summary: The study demonstrates the potential of using physically pre-treated Agave durangensis leaves for PHB production, leading to increased biosynthesis and improved thermal stability of the polymer. These results promote the development of a circular economy in Mexico.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Article Biotechnology & Applied Microbiology

Monitoring pilot-scale polyhydroxyalkanoate production from fruit pulp waste using near-infrared spectroscopy

Beatriz Sousa et al.

Summary: This study produced PHA from fermented fruit pulp waste and monitored the production process in real-time using NIR spectroscopy and PLS calibration models. The calibration models showed good performance and offered a potential solution for the current plastic disposal crisis with the use of biodegradable polymers.

BIOCHEMICAL ENGINEERING JOURNAL (2021)

Article Biochemistry & Molecular Biology

Biodegradable polyhydroxyalkanoates production from wheat straw by recombinant Halomonas elongata A1

Changli Liu et al.

Summary: The bio-transformation of waste straw and waste oil to synthesize PHA materials plays a significant role in environmental protection and sustainable development, and PHA synthesized from different substrates exhibits different physical properties, which contributes to the improvement of biodegradable plastic products synthesis.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Article Biochemistry & Molecular Biology

Screening of the strictly xylose-utilizing Bacillus sp. SM01 for polyhydroxybutyrate and its co-culture with Cupriavidus necator NCIMB 11599 for enhanced production of PHB

Sun Mi Lee et al.

Summary: This study screened and characterized ten xylose-utilizing PHB producers isolated from South Korean marine environments. Among these isolates, a newly identified strain Bacillus sp. SM01 produced the highest amount of PHB using xylose. Co-culturing Bacillus sp. SM01 with Cupriavidus necator NCIMB 11599 resulted in increased PHB production and overcome the limitation of sugar consumption associated with Bacillus sp. SM01 and C. necator.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Review Microbiology

Genome-Wide Metabolic Reconstruction of the Synthesis of Polyhydroxyalkanoates from Sugars and Fatty Acids by Burkholderia Sensu Lato Species

Natalia Alvarez-Santullano et al.

Summary: Burkholderia sensu lato (s.l.) species exhibit versatile metabolism in synthesizing polyhydroxyalkanoates (PHAs) from sugars and fatty acids. Genomic analysis showed that all Burkholderia s.l. strains possess the phaC, phaA, and phaB genes for PHA synthesis with a high level of gene redundancy. The PHA synthases were classified into four phylogenetic groups, reflecting complex regulatory mechanisms for fine-tuning synthesis based on diverse substrates and physiological conditions.

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Article Biotechnology & Applied Microbiology

Polyhydroxyalkanoate production from rice straw hydrolysate obtained by alkaline pretreatment and enzymatic hydrolysis using Bacillus strains isolated from decomposing straw

Doan Van Thuoc et al.

Summary: Rice straw is an important low-cost feedstock for bio-based economy, and the study demonstrates that alkaline pretreatment and enzymatic hydrolysis of rice straw can lead to the production of PHA by isolated bacteria, which helps increase PHA yield.

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Lucia Salamanca-Cardona et al.

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