New perspective on effect of beta-cyclodextrin on nitrification-denitrification and denitrification phosphorus removal in biogenic manganese oxides driven moving bed biofilm reactor: Performance evaluation, microbial community and process

Effect of beta-cyclodextrin (beta-CD) on simultaneous removal of NH4+-N, NO3--N, COD, and phosphorus (P) in biogenic manganese oxides (BioMnOx) driven moving bed biofilm reactor (MBBR) was investigated. 58.64% and 86.32%, 79.65% and 98.39%, 62.45% and 97.30%, and 24.80% and 95.90% of TN and COD were removed in phases I-IV, indicating that simultaneous nitrification and denitrification (SND) efficiencies were 75.44%, 83.91%, 72.71%, and 35.83%, respectively. Composition and fluorescence spectral characteristics of extracellular polymeric substance (EPS) were evaluated including the removal kinetics of TN and COD. Metabolic activity of Mn2+, decolorization performance of BioMnOx, and reactive oxygen species (ROS) characteristics were determined in biofilm. Furthermore, intermediate Mn3+ and BioMnOx concentration were analyzed. Finally, the removal process of nitrogen (N) and P was proposed based on characterizations of elemental characterization, electro-chemistry, and microbial community. This study provides new insights into the N and P removal mediated by BioMnOx and beta-CD.

Automated summary

The study investigated the simultaneous removal of NH4+-N, NO3--N, COD, and P in a biogenic manganese oxides (BioMnOx) driven moving bed biofilm reactor (MBBR) with the addition of beta-cyclodextrin (beta-CD). The results showed that TN and COD removal efficiencies in phases I-IV were 58.64% and 86.32%, 79.65% and 98.39%, 62.45% and 97.30%, and 24.80% and 95.90%, respectively. The study also evaluated the characteristics of extracellular polymeric substance (EPS), the metabolic activity of Mn2+, decolorization performance of BioMnOx, and reactive oxygen species (ROS) characteristics. Furthermore, the study proposed a removal process of nitrogen and phosphorus based on elemental characterization, electro-chemistry, and microbial community.