Achieving high enzymatic hydrolysis sugar yield of sodium hydroxide-pretreated wheat straw with a low cellulase dosage by adding sulfomethylated tannic acid

Sulfonated lignin can significantly enhance the enzymatic hydrolysis of lignocellulose substrates. Lignin is a type of polyphenol, therefore, sulfonated polyphenol, such as tannic acid, is likely to have similar effects. In order to obtain a low-cost and high-efficiency additive to improve enzymatic hydrolysis, sulfomethylated tannic acids (STAs) with different sulfonation degrees were prepared and their impact on enzymatic saccharification of sodium hydroxide-pretreated wheat straw were investigated. Tannic acid strongly inhibited, while STAs strongly promoted the substrate enzymatic digestibility. While adding 0.04 g/g-substrate STA containing 2.4 mmol/g sulfonate group, the glucose yield increased from 60.6% to 97.9% at a low cellulase dosage (5 FPU/g-glucan). The concentration of protein in enzymatic hydrolysate significantly increased with the added STAs, indicating that cellulase preferentially adsorbed with STAs, thereby reducing the amount of cellulase nonproductively anchored on substrate lignin. This result provides a reliable approach for establishing an efficient lignocellulosic enzyme hydrolysis system.

Automated summary

Sulfonated lignin enhances enzymatic hydrolysis of lignocellulose substrates. Sulfonated polyphenols, such as tannic acid, may have similar effects. Sulfomethylated tannic acids (STAs) with different sulfonation degrees were prepared and investigated for their impact on enzymatic saccharification. STAs significantly promoted the substrate enzymatic digestibility, providing a reliable approach for establishing an efficient lignocellulosic enzyme hydrolysis system.