Enhanced ethanol-driven carboxylate chain elongation by Pt@C in simulated sequencing batch reactors: Process and mechanism

Carboxylate chain elongation can create value-added bioproducts from the organic waste. The effects of Pt@C on chain elongation and associated mechanisms were investigated in simulated sequencing batch reactors. 5.0 g/L of Pt@C greatly increased the synthesis of caproate, with an average yield of 21.5 g COD/L, which was 207.4% higher than the trial without Pt@C. Integrated metagenomic and metaproteomic analyses were used to reveal the mechanism of Pt@C-enhanced chain elongation. Pt@C enriched chain elongators by increasing the relative abundance of dominant species by 115.5%. The expression of functional genes related to chain elongation was promoted in the Pt@C trial. This study also demonstrates that Pt@C may promote overall chain elongation metabolism by enhancing CO2 uptake of Clostridium kluyveri. The study provides insights into the fundamental mechanisms of how chain elongation can perform CO2 metabolism and how it can be enhanced by Pt@C to upgrade bioproducts from organic waste streams.

Automated summary

This study investigates the effects of Pt@C on chain elongation and its associated mechanisms in simulated sequencing batch reactors. The addition of 5.0 g/L of Pt@C greatly enhances the synthesis of caproate, with an average yield of 21.5 g COD/L, which is 207.4% higher than the trial without Pt@C. Integrated metagenomic and metaproteomic analyses reveal that Pt@C enhances chain elongation by increasing the relative abundance of dominant species and promoting the expression of functional genes related to chain elongation. Furthermore, Pt@C may enhance CO2 uptake of Clostridium kluyveri to promote overall chain elongation metabolism. This study provides insights into the fundamental mechanisms of chain elongation and its enhancement by Pt@C in upgrading bioproducts from organic waste streams.