期刊
BIORESOURCE TECHNOLOGY
卷 383, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2023.129231
关键词
Omega-6 polyunsaturated fatty acids; Gamma-linolenic acid; Dihomo-gamma-linolenic acid; Arachidonic acid; Yarrowia lipolytica
This study explored the optimal biosynthetic pathways for customized production of co6-PUFAs in Y. lipolytica and effectively increased the proportions of co6-PUFAs in total fatty acids through various strategies. The customized strains synthesized GLA, DGLA, and ARA with high titers, providing valuable insights into the production of functional co6-PUFAs.
Omega-6 polyunsaturated fatty acids (co6-PUFAs), such as y-linolenic acid (GLA), dihomo-y-linolenic acid (DGLA) and arachidonic acid (ARA), are indispensable nutrients for human health. Harnessing the lipogenesis pathway of Yarrowia lipolytica creates a potential platform for producing customized co6-PUFAs. This study explored the optimal biosynthetic pathways for customized production of co6-PUFAs in Y. lipolytica via either the A6 pathway from Mortierella alpina or the A8 pathway from Isochrysis galbana. Subsequently, the proportion of co6-PUFAs in total fatty acids (TFAs) was effectively increased by bolstering the provision of precursors for fatty acid biosynthesis and carriers for fatty acid desaturation, as well as preventing fatty acid degradation. Finally, the proportions of GLA, DGLA and ARA synthesized by customized strains accounted for 22.58%, 46.65% and 11.30% of TFAs, and the corresponding titers reached 386.59, 832.00 and 191.76 mg/L in shake-flask fermentation, respectively. This work provides valuable insights into the production of functional co6-PUFAs.
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