Novel supramolecular deep eutectic solvent-enabled in-situ lignin protection for full valorization of all components of wheat straw

Lignin is usually deemed as an inhibitor to enzymatic hydrolysis of cellulose due to its physical barrier, nonproductive adsorption, and steric hindrance. Herein, a novel supramolecular deep eutectic solvent (SUPRADES), comprising ethylene glycol and citric acid in 5:1 M ratio, and beta-cyclodextrin (beta-CD) in a concentration of 3.5% (w/w), was developed to be efficient for pretreating wheat straw. The delignification rate, cellulose enzymatic digestibility, and hemicellulose removal reached 90.45%, 97.36% and 87.24%, respectively, which may be attributed to the introduction of beta-CD with superior ability of both adsorption and in-situ lignin protection to efficiently remove lignin with intact structure from cellulose surface. The mechanisms of high-efficiency lignin extraction/protection were systematically illustrated by adsorption kinetics. Moreover, Trichosporon cutaneum grown on the hemicellulose and cellulose fractions after pretreatment afforded 8.8 g total lipids from 100 g wheat straw. The green SUPARDES pretreatment strategy offers a new avenue for upgrading lignocellulose to biofuels.

Automated summary

A novel supramolecular deep eutectic solvent (SUPRADES) was developed for efficient pretreatment of wheat straw, which can effectively remove lignin and protect cellulose, thereby improving cellulose enzymatic digestibility and hemicellulose removal. This green pretreatment strategy offers a new avenue for upgrading lignocellulose to biofuels.