☆ 4.5 Review

A critical review of enzymes immobilized on chitosan composites: characterization and applications

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2023)

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Article Chemistry, Applied

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

Xin Gao et al.

Summary: D-allulose 3-epimerase (DAEase) from Clostridium cellulolyticum was successfully immobilized via covalent attachment (RI-CcDAE) and modular approach (DI-CcDAE). Both immobilized CcDAEs showed improved thermal and pH stabilities compared to the free enzyme, maintaining 80.0% relative activity after 7 cycles and 25 days of storage. DI-CcDAE demonstrated superior catalytic efficiency with a 2.4-fold increase in k(cat)/K-m compared to RI-CcDAE. Application of RI-CcDAE and DI-CcDAE in fruit Jiaosu resulted in the conversion of D-fructose into D-allulose with productivities of 1.08 g/Lh(-1) and 1.57 g/Lh(-1) respectively. This research provides a promising strategy for directional immobilization of DAEase and robust biocatalyst for producing functional foodstuff containing D-allulose.

FOOD CHEMISTRY (2023)

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Article Food Science & Technology

Graphitic carbon nitride/magnetic chitosan composite for rapid electrochemical detection of lactose

Hasan Nasiri et al.

Summary: Graphitic carbon nitride supported on magnetic chitosan was synthesized and used as a support for cellobiose dehydrogenase immobilization and as a modifier for glassy carbon electrode in the fabrication of an electrochemical lactose sensor. The composite exhibited excellent electrochemical performance and was successfully applied in lactose quantification in dairy products.

INTERNATIONAL DAIRY JOURNAL (2023)

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Article Biochemical Research Methods

A general strategy for protein affinity-ligand oriented-immobilization and screening for bioactive compounds

Yu Yi et al.

Summary: In this study, a facile and efficient protein affinity-ligand oriented-immobilization strategy based on SpyTag/SpyCatcher chemistry was developed for bioactive compound screening. The feasibility of this method was verified using GFP and PqsA as model proteins. The results showed that this strategy can immobilize protein ligands in one-step and screen compounds that interact specifically with the ligands.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2023)

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Article Chemistry, Multidisciplinary

Immobilization of laccase on carboxyl-functionalized chitosan-coated magnetic nanoparticles with improved stability and reusability

Houman Maftoon et al.

Summary: In this study, carboxyl-functionalized chitosan-coated magnetic nanoparticles were synthesized and used for the immobilization of laccase enzyme. The immobilized laccase showed improved catalytic activity and stability, with higher activity at higher temperatures and better storage stability and reusability compared to the free enzyme.

MONATSHEFTE FUR CHEMIE (2023)

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Review Multidisciplinary Sciences

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Hilda Dinah Kyomuhimbo et al.

Summary: Laccase is a multi-copper enzyme that can directly reduce molecular oxygen to water without producing hydrogen peroxide. It has non-specificity and uses molecular oxygen as a secondary substrate, making it attractive for biotechnological applications. This review discusses the diverse applications of laccase in food, paper and pulp, wastewater treatment, pharmaceuticals, sensors, and fuel cells. Challenges such as high cost, instability in harsh environmental conditions, and proteolysis can be mitigated through immobilization, which is extensively discussed in this review.

HELIYON (2023)

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Article Chemistry, Applied

Construction of porous chitosan macrospheres via dual pore-forming strategy as host for alkaline protease immobilization with high activity and stability

Ying Tang et al.

Summary: Fabrication of chitosan macro-spheres with interconnected and interlaced network pores through a dual pore-forming strategy enables effective immobilization of alkaline protease. The resulting porous support P2CSM exhibits high loading capacity and activity recovery for alkaline protease. Protease@P2CSM demonstrates superior structural stability, reusability, SDS-resistance, and proteolytic ability, making it a promising enzyme-composite for practical applications.

CARBOHYDRATE POLYMERS (2023)

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Article Chemistry, Physical

Optimization of Biocatalytic Steps via Response Surface Methodology to Produce Immobilized Peroxidase on Chitosan-Decorated AZT Composites for Enhanced Reusability and Storage Stability

Yaaser Q. Almulaiky et al.

Summary: The aim of this study was to investigate and identify the optimal conditions for immobilizing peroxidase on chitosan-decorated alumina-zirconia-titania composites (AZT) activated with glutaraldehyde. Response surface methodology was used to optimize the immobilization conditions, and the results showed that the best immobilization efficiency and enzyme activity were achieved with 40 mg of AZT composites, 1.5% glutaraldehyde, 200 units of enzyme, and a pH of 6.5. The mineralogical structure of the prepared AZT and CS/AZT was studied using X-ray diffraction patterns, while scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy were used to analyze the morphological and chemical properties of the immobilized peroxidase on CS/AZT. The study also found that the immobilized peroxidase exhibited a residual activity of 85% after 15 repetitions, and the residual activity of both immobilized and free enzymes after 12 weeks of storage was 81% and 32% of their initial activity, respectively. Based on these findings, it can be concluded that peroxidase immobilization technology is promising for industrial applications.

CATALYSIS LETTERS (2023)

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Article Biochemistry & Molecular Biology

Immobilization of lipase in chitosan-mesoporous silica material and pore size adjustment

Xiaonan Zhou et al.

Summary: In this study, carboxyl-modified mesoporous silica MCM-41 was assembled with chitosan to create a novel nanocarrier for the immobilization of lipase. The composite was further modified with silane coupling agent KH560 to decrease pore size and prevent enzyme loss. The lipase fixed in the CTS-MCM-41 carrier showed a residual activity of 85% after seven repeated use cycles. The optimal conditions for enzyme immobilization involved a carrier to enzyme mass ratio of 4:1, 3% glutaraldehyde solution volume, 3-hour reaction time, and 45°C reaction temperature. Overall, the proposed innovative carrier allows for stable immobilization of lipase and offers potential for large-scale industrial production in an efficient miniature reactor.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

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Article Biochemistry & Molecular Biology

Study of stability, kinetic parameters and release of lysozyme immobilized on chitosan microspheres by crosslinking and covalent attachment for cotton fabric functionalization

Annie Ceron et al.

Summary: Chitosan microspheres containing lysozyme were synthesized using crosslinking and covalent attachment methods. The synthesis conditions were optimized, and the microspheres were characterized by SEM and ATR-FTIR. The influence of immobilization methods on enzymatic activity and stability were evaluated, and the enzymatic activity recovery yields were determined. The functionalization of cotton fabric with lysozyme-chitosan microspheres resulted in slight modifications to fabric properties and demonstrated antimicrobial properties against S. aureus and P. aeruginosa.

PROCESS BIOCHEMISTRY (2023)

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Article Materials Science, Multidisciplinary

Purification of alkaline phosphatase from bovine milk through metal ion affinity by a novel magnetic nanocomposite based on functionalized chitosan with dopamine and nickel

Reza Eivazzadeh-Keihan et al.

Summary: The use of magnetic functionalized chitosan-based nanocarrier for protein and enzyme purification is convenient and rapid. Surface modification of chitosan with terephthaloyl chloride, dopamine, and NiCl2 followed by magnetization with Fe3O4 nanoparticles resulted in a magnetic nanocomposite. This nanocomposite was successfully utilized for the separation of alkaline phosphatase from bovine milk with a purification yield of 67%.

MATERIALS TODAY COMMUNICATIONS (2023)

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Article Chemistry, Physical

Dihydroflavonol 4-reductase immobilized on Fe3O4-chitosan nanoparticles as a nano-biocatalyst for synthesis of anthocyanidins

Xuefeng Li et al.

Summary: In this study, we successfully immobilized recombinant DFR protein onto Fe3O4-Chitosan nanocarrier and characterized its properties. The immobilized enzyme showed enhanced thermal and acid stabilities and tolerated inhibitions from Zn2+, Cu2+, Fe2+, and various organic solvents. However, the catalytic yield of anthocyanidins was relatively low. This work developed a new recyclable nano-immobilized biocatalyst for anthocyanidins synthesis.

CHEMICAL PHYSICS LETTERS (2023)

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Article Chemistry, Inorganic & Nuclear

A novel effective immobilization of glucose oxidase on Ni0.25Zn0.25Cu0.25Co0.25La0.06Fe1.94O4-Chitosan nanocomposite as an enzymatic glucose biosensor

S. Deepapriya et al.

Summary: In recent years, there has been significant interest in enzymatic glucose biosensors for continuous glucose monitoring. To meet the demand for accurate and reliable glucose biosensors, a novel Ni0.25Zn0.25Cu0.25Co0.25La0.06Fe1.94O4 nanocomposite was synthesized through the combustion method. This nanocomposite was used to develop an effective glucose biosensor by immobilizing glucose oxidase onto chitosan-Ni0.25Zn0.25Cu0.25Co0.25La0.06Fe1.94O4 hetero-junction nanocomposite on FTO glass substrate.

INORGANIC CHEMISTRY COMMUNICATIONS (2023)

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Article Chemistry, Analytical

Chitosan-based Maillard products for enzyme immobilization in multilayers structure: Its application in electrochemical sensing

Florencia Alejandra Gulotta et al.

Summary: In this study, water-soluble chitosan polycation obtained by Maillard reaction with glucose (G-Chit) or lactose residues was used for the immobilization of glucose oxidase (GOx) and lactate oxidase onto carbon paste electrodes using the layer-by-layer adsorption method. The G-Chit and GOx multilayer structure showed the best performance, with a sensitivity of (12.4 +/- 0.3) mu A mM-1 cm-2 in a glucose concentration range from 180 μM to 1.75 mM. The biosensor exhibited fast responses for quantification of glucose even in milk samples, confirming the suitability of these chitosan derivatives for enzyme adsorption and supramolecular structure construction.

MICROCHEMICAL JOURNAL (2023)

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Article Biotechnology & Applied Microbiology

Glutaraldehyde-copper gelled chitosan beads: Characterization and utilization as covalent immobilizers

Marwa I. Wahba

Summary: CHS beads were prepared by utilizing the strong chitosan (CHS)-Cu2+ interaction, and glutaraldehyde (GA) was added to the gelling medium for β-galactosidase (βG) covalent immobilization. The concentrations of CuSO4, CHS, and GA solutions were optimized for the preparation of GA-Cu-CHS beads, with a CHS concentration of 2% and the optimal gelling medium comprising 0.2% CuSO4-1.12% GA. The beads were characterized and showed enhanced mechanical properties.

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY (2023)

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Review Materials Science, Paper & Wood

Sources, production and commercial applications of fungal chitosan: A review

Tanzina Huq et al.

Summary: Chitosan, a biopolymer, can be obtained from animal/marine sources or derived from agricultural waste like mushrooms or various fungi through chitin deacetylation. The cell walls of filamentous fungi provide an easy method for chitin extraction, allowing for control over the physicochemical characteristics of fungal chitosan compared to chitosan from crustacean sources. Fungal sourced chitosan finds applications in food, pharmaceutical, and biomedical fields, serving as an antimicrobial agent, coating material, water purification agent, or bio-pesticide. This review focuses on the extraction of chitin from mushrooms or different fungal sources, as well as highlighting various applications of commercial chitosan products.

JOURNAL OF BIORESOURCES AND BIOPRODUCTS (2023)

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Article Biochemistry & Molecular Biology

The Neutral Protease Immobilization: Physical Characterization of Sodium Alginate-Chitosan Gel Beads

Yuan Bai et al.

Summary: In this study, composite gel spheres were formed by cross-linking sodium alginate and chitosan to immobilize neutral protease. The immobilized enzyme exhibited high relative activity under various conditions and showed good stability in terms of pH and temperature. These findings suggest that the immobilized neutral protease is a promising alternative to the free enzyme.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022)

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Article Chemistry, Applied

Recent advances in extraction, modification, and application of chitosan in packaging industry

Nazila Oladzadabbasabadi et al.

Summary: Chitosan, a renewable material with good biodegradability and natural availability, shows great potential as a food packaging material to extend the shelf-life and maintain the qualities of food commodities. Chitosan-reinforced composites also exhibit antimicrobial, antioxidant, and antifungal activities, making them versatile for various applications.

CARBOHYDRATE POLYMERS (2022)

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Article Engineering, Chemical

Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies

Katerine da S. Moreira et al.

Summary: In this study, co-immobilization of lipase A and lipase B from Candida antarctica in glutaraldehyde-activated chitosan was optimized using the Taguchi method, achieving a high immobilization yield and activity. The CALA-CALB-CHI derivative showed a longer half-life and higher catalytic activity compared to the soluble enzyme mixture under different pH conditions. Additionally, the immobilized derivative demonstrated excellent performance in the kinetic resolution of halohydrins acetates, producing enantiomerically enriched products that are important intermediates in drug synthesis.

CHEMICAL ENGINEERING RESEARCH & DESIGN (2022)

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Article Agricultural Engineering

One-step solid-state fermentation for efficient erythritol production from the simultaneous saccharified crop wastes by incorporating immobilized cellulase

Xiaoyan Liu et al.

Summary: The study introduced a one-step solid-state fermentation method for erythritol production from oil crop waste, utilizing immobilized cellulase for simultaneous saccharification, resulting in enhanced erythritol production with high stability and reusability.

INDUSTRIAL CROPS AND PRODUCTS (2022)

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Article Materials Science, Multidisciplinary

Concurrent electrophoretic deposition of enzyme-laden chitosan/graphene oxide composite films for biosensing

Fereshteh Moharramzadeh et al.

Summary: The study presents a method for simultaneous deposition of enzyme-laden chitosan/rGO films using electrophoretic deposition (EPD) for fast and efficient glucose detection. The performance of the biosensor shows high sensitivity, low limit of detection, and suitable selectivity as indicated by electrochemical techniques.

MATERIALS LETTERS (2022)

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Article Polymer Science

Development of PAMAM dendrimer-modified magnetic chitosan: a novel platform for α-amylase immobilization

Bindu Vadakethil Unniganapathi et al.

Summary: The study synthesized two generations of PAMAM-modified magnetic chitosan as enzyme carriers, with high immobilization efficiency using adsorption and covalent binding methods. The immobilized enzymes showed stability over a wide pH range and optimal activity at higher temperatures. The carriers exhibited thermal stability, high affinity, improved activity, and ease of handling through magnetic separation, making them suitable for various industrial applications.

POLYMER BULLETIN (2022)

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Review Chemistry, Multidisciplinary

Insights into recent advances of chitosan-based adsorbents for sustainable removal of heavy metals and anions

Ahmed M. Omer et al.

Summary: With the global increase in population and industrialization, natural water sources are becoming scarce and contaminated, making the development of efficient water treatment technologies a priority for all nations. This review focuses on the recent advancements in chitosan-based adsorbents for the removal of heavy metals and anions, discussing the adsorption mechanisms, optimal conditions, and research gaps for future studies. The study aims to inspire further research to find the best solutions for water treatment issues.

ARABIAN JOURNAL OF CHEMISTRY (2022)

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Article Chemistry, Multidisciplinary

Enzyme immobilization strategies for the design of robust and efficient biocatalysts

Jose M. Guisan et al.

Summary: This review discusses different strategies for the preparation of efficient and robust immobilized biocatalysts, including immobilization on pre-existing porous supports, multipoint covalent attachment on support surfaces, and additional stabilization through physical or chemical modification with polymers. These strategies can be easily developed and result in stabilizations ranging between 1000 and 100,000 folds compared to diluted soluble enzymes.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2022)

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Review Biotechnology & Applied Microbiology

Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules

Meena Nemiwal et al.

Summary: Biosensors have emerged as important tools for selective and sensitive detection of biomolecules. Enzymes are widely used as recognition sites in biosensors, but their immobilization remains challenging. Researchers are exploring the use of functionalized nanomaterials/nanoparticles to enhance the performance of biosensors.

ENZYME AND MICROBIAL TECHNOLOGY (2022)

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Article Polymer Science

Chitosan-based drug delivery systems: Current strategic design and potential application in human hard tissue repair

Zhenqi Liu et al.

Summary: Chitosan, a natural polysaccharide, has been extensively studied as a bioactive carrier in drug delivery systems (DDSs) due to its excellent biocompatibility and biodegradability. By combining with other materials and bioactive molecules, chitosan-based DDSs have been utilized for promoting remineralization and osteogenesis, making them suitable for human hard tissue repair.

EUROPEAN POLYMER JOURNAL (2022)

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Article Chemistry, Applied

Chitosan for food packaging: Recent advances in active and intelligent films

Maria Florez et al.

Summary: The massive use of petroleum-based plastics in food packaging has led to serious environmental issues, prompting the need to develop biodegradable polymer packaging. Chitosan, a natural polysaccharide with non-toxic, antimicrobial, and antifungal properties, is considered a promising material for food packaging. Recent studies have evaluated the potential of active and/or intelligent chitosan-based films in improving food preservation and monitoring. The addition of active compounds has been shown to enhance the mechanical, barrier and functional properties of chitosan films, with potential applications in antimicrobial and antioxidant activities.

FOOD HYDROCOLLOIDS (2022)

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Article Biochemistry & Molecular Biology

Post-hydrolysis of cellulose oligomers by cellulase immobilized on chitosan-grafted magnetic nanoparticles: A key stage of butanol production from waste textile

Armaghan Javid et al.

Summary: The recently developed immobilization technologies for cellulase can address the cost challenges in cellulose hydrolysis for cellulosic butanol production. A hybrid hydrolysis technique was developed in this study, based on chemical hydrolysis followed by enzymatic post-hydrolysis using immobilized cellulase on chitosan-coated Fe3O4 nanoparticles. This hybrid hydrolysis was successfully used in the production of biobutanol from jeans waste, resulting in selective formation of glucose and high yield of butanol production.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Biochemistry & Molecular Biology

Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies

Shalu Aggarwal et al.

Summary: In this study, trypsin was effectively immobilized on the surface of ZnO/Chitosan nanocomposite through covalent attachment. The size, structure, surface morphology, and elemental composition of the nanoparticles and nanocomposite were studied. Optimal immobilization conditions and catalytic activity were investigated. The results showed improved efficiency and stability of the immobilized trypsin, suggesting its potential application in biotechnological processes.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Review Biochemistry & Molecular Biology

A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

Roberto Morellon-Sterling et al.

Summary: This article highlights the complexities of immobilizing pepsin and discusses potential methods and applications. The lack of primary amino groups on the enzyme's surface and its poor stability at alkaline pH values present challenges for immobilization. The review also proposes possibilities for achieving both covalent immobilization and stabilization of pepsin through multipoint covalent attachment.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Food Science & Technology

Thermostable Chitosan-L-Asparaginase conjugate from Aspergillus fumigatus is a novel structurally stable composite for abolishing acrylamide formation in French fried potatoes

Marwa A. Yassin et al.

Summary: The objective of this study was to purify highly active thermostable Asnase from fungi and increase its structural stability for acrylamide reduction by conjugating it with biocompatible polymers. The results showed that chitosan-Af-Asnase conjugates exhibited high structural stability and efficiency for abolishing acrylamide formation.

LWT-FOOD SCIENCE AND TECHNOLOGY (2022)

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Review Biochemistry & Molecular Biology

Enzyme immobilization: Implementation of nanoparticles and an insight into polystyrene as the contemporary immobilization matrix

Shivam Sharma et al.

Summary: Enzyme immobilization has various applications in industrial and production activities. The use of nanoparticles as carriers can enhance the stability and activity of immobilized enzymes. Polystyrene surface as a support material for enzyme immobilization offers numerous advantages.

PROCESS BIOCHEMISTRY (2022)

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Article Chemistry, Applied

Comparative affinity immobilization of α-galactosidase on chitosan functionalized with Concanavalin A and its useability for the hydrolysis of raffinose

Esin Calci et al.

Summary: This study presents a new strategy for enzyme immobilization, called bioaffinity immobilization, which utilizes biospecific affinity interactions between enzymes and matrices. The authors immobilized alpha-galactosidase on chitosan functionalized with Concanavalin A using three different methods. The results showed that each method had different immobilization yields and activity levels, with method II achieving the highest immobilization yield and method III exhibiting the greatest hydrolysis efficiency for raffinose. The immobilized enzymes also demonstrated promising biochemical characterization and stability, indicating potential applications in the food and feed industries.

REACTIVE & FUNCTIONAL POLYMERS (2022)

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Article Green & Sustainable Science & Technology

Phosphomolybdic acid/chitosan as acid solid catalyst using for biodiesel production from pomegranate seed oil via microwave heating system: RSM optimization and kinetic study

Fatemeh Helmi et al.

Summary: In this research, a solid catalyst of phosphomolybdic acid (HPA) prated on Chitosan was synthesized and used in the production of high-quality Pomegranate biodiesel through microwave-assisted transesterification. The catalyst showed good reusability and has great potential for large-scale biodiesel production.

RENEWABLE ENERGY (2022)

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Review Chemistry, Multidisciplinary

Sustainable adsorptive removal of antibiotic residues by chitosan composites: An insight into current developments and future recommendations

Eman M. Abd El-Monaem et al.

Summary: This article provides a comprehensive overview of recent research on the removal of antibiotics by chitosan composite-based adsorbents. The structure, optimal removal conditions, and adsorption mechanisms of various antibiotics are summarized. The development of chitosan composite-based adsorbents and the factors influencing the adsorption process are discussed. Conclusions and future recommendations are also provided.

ARABIAN JOURNAL OF CHEMISTRY (2022)

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Review Energy & Fuels

Biotechnological advances in biomass pretreatment for bio-renewable production through nanotechnological intervention

Heena Chandel et al.

Summary: This article surveys different biomass pretreatment methods and explores nanotechnological interventions to enhance the production efficiency and economic viability of biofuels and other by-products.

BIOMASS CONVERSION AND BIOREFINERY (2022)

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Article Engineering, Environmental

Decolorization and detoxification of textile effluent by immobilized laccase-ACS into chitosan-clay composite beads using a packed bed reactor system: An ecofriendly approach

Seema Mehandia et al.

Summary: In this study, a bacterial laccase from Alcaligenes faecalis XF1 was immobilized into chitosan-clay composite beads to form an immobilized laccase mediator system (CCB-L-ACS). The CCB-L-ACS system effectively decolorized textile effluent and reduced BOD and COD levels. Micro-toxicity assay confirmed a significant decrease in toxicity after treatment with the CCB-L-ACS system.

JOURNAL OF WATER PROCESS ENGINEERING (2022)

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Article Biotechnology & Applied Microbiology

Development and applications of nanobiosensors for sustainable agricultural and food industries: Recent developments, challenges and perspectives

Meenu Thakur et al.

Summary: The increasing global population and limited natural resources pose major challenges to the sustainability of agricultural and food industries. The application of nanobiosensors can enhance resource utilization efficiency and help address these challenges.

ENVIRONMENTAL TECHNOLOGY & INNOVATION (2022)

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Article Biochemistry & Molecular Biology

Enzymatic synthesis of sodium caseinate-EGCG-carboxymethyl chitosan ternary film: Structure, physical properties, antioxidant and antibacterial properties

Qiankun Wang et al.

Summary: Proteins and polysaccharides are commonly used to prepare eco-friendly packaging materials. However, films based on proteins or polysaccharides alone often have poor performance, so combining them is necessary to improve properties. In this study, enzyme technology was used to prepare films with sodium caseinate-carboxymethyl chitosan, incorporating epigallocatechin gallate as bridging molecules and antibacterial agents. The films showed improved mechanical properties, antibacterial activity, and free radical scavenging ability with the increase of epigallocatechin gallate and tyrosinase content. This work provides an efficient enzymatic method to prepare films with good strength and antibacterial properties for improving food storage quality.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Review Chemistry, Applied

Chitosan nanomaterials: A prelim of next-generation fertilizers; existing and future prospects

Damyanti Prajapati et al.

Summary: Global agriculture is searching for ways to mitigate the negative effects of conventional chemical fertilizers on the environment. Chitosan nanomaterials have shown promise as next-generation fertilizers, improving nutrient use efficiency and reducing the environmental footprint.

CARBOHYDRATE POLYMERS (2022)

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Article Engineering, Environmental

Laccase immobilized on chitosan-polyacrylic acid microspheres as highly efficient biocatalyst for naphthol green B and indigo carmine degradation

Anca Ruxandra Leonties et al.

Summary: Low-cost and non-toxic microspheres made of chitosan and polyacrylic acid were used as carriers for laccase enzyme. The microspheres' properties were influenced by the ratio of polyacrylic acid and played a role in enzyme immobilization. The product with the highest enzyme activity and stability was 10CsPAALc(3), which also showed faster degradation of model dyes.

CHEMICAL ENGINEERING JOURNAL (2022)

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Article Engineering, Chemical

Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance

T.A. Costa-Silva et al.

CHEMICAL ENGINEERING RESEARCH & DESIGN (2022)

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Article Environmental Sciences

Catalyzed degradation of polycyclic aromatic hydrocarbons by recoverable magnetic chitosan immobilized laccase from Trametes versicolor

Jibao Deng et al.

Summary: This study immobilized laccase on magnetic particles to improve its operational stability and reusability in the treatment of PAH pollution. The immobilized laccase showed high degradation efficiencies for anthracene and benzo[a]pyrene. The findings provide valuable insight in promoting the application of immobilized laccase technology in the remediation of PAH contamination.

CHEMOSPHERE (2022)

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Review Chemistry, Analytical

Chitosan- and/or cellulose-based materials in analytical extraction processes: A review

Ahmad Reza Bagheri et al.

Summary: The application of bio-based materials in analytical chemistry has become increasingly important. This article reviews recent advancements in the use of chitosan (CS), cellulose (CL), and modified CS-/CL-based materials (CSCLBMs) as sorbents in analytical sample preparation methods. The article discusses functionalization and modification of CS or CL with different materials and provides guidance on the capability of CSCLBMs as sorbents. The review also summarizes the current challenges, future trends, and directions in relation to these materials in extraction techniques.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

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Review Biochemistry & Molecular Biology

Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects - A review

Camila S. Sampaio et al.

Summary: This review focuses on the preparation and application of cross-linked enzyme aggregates (CLEAs) from lipases. CLEAs offer advantages such as simplicity, the ability to use crude enzyme extracts, and stability under extreme conditions. However, there are challenges in the preparation process, which can be addressed through improved cross-linking methods and the design of magnetic-CLEAs.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Environmental Sciences

Enzyme-loaded nanostructured materials for the degradation of environmental pollutants

Mahpara Qamar et al.

Summary: Nanomaterials are commonly used for enzyme immobilization, providing desired functionality and reducing the cost of bioprocess. Immobilization technology protects the structure of enzymes, making them more stable. Enzyme-loaded nanocarriers can be recovered and reused in multiple cycles.

CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH (2022)

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Review Agricultural Engineering

Advances in bioremediation of emerging contaminants from industrial wastewater by oxidoreductase enzymes

P. Sai Preethi et al.

Summary: The use of enzyme biotechnology for the bioremediation of emerging pollutants in wastewater offers a cost-effective and low-energy approach. However, this technology is still not fully developed for commercial use. Oxidoreductases, as dominant biocatalysts, show promise for wastewater treatments. Therefore, understanding their global market and biotransformation efficacy is essential for establishing these bio-enzymes in commercial scale. Combining the biocatalytic strategy with existing treatment technology can achieve effective removal of emerging pollutants from wastewater. This review provides insights into the treatment of toxicological xenobiotics from various industries and the potential of peroxidases, nitroreductase, and cellobiose dehydrogenase in their bio-based treatment. Additionally, it comprehensively covers the environmental relevance of wastewater pollution and the challenges faced in terms of biocatalytic remediation for future prospects.

BIORESOURCE TECHNOLOGY (2022)

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Article Biotechnology & Applied Microbiology

Cellulose- and xylan-degrading yeasts: Enzymes, applications and biotechnological potential

Katarína Šuchová et al.

BIOTECHNOLOGY ADVANCES (2022)

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Article Chemistry, Applied

Novel chitosan based metal-organic polyhedrons/enzyme hybrid hydrogel with antibacterial activity to promote wound healing

Jie Song et al.

Summary: This study developed a novel chitosan based metal-organic polyhedrons (MOPs)/enzyme hybrid hydrogel with superior antimicrobial properties for wound healing treatment. The hydrogel exhibited excellent bactericidal effect on both gram-negative and gram-positive bacteria, and also showed good cell compatibility. In vivo experiments demonstrated that the hydrogel effectively promoted wound healing. These findings suggest that chitosan based MOPs/enzyme hybrid hydrogel holds great potential for antibacterial therapy in clinical applications.

CARBOHYDRATE POLYMERS (2022)

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Review Biophysics

Design of composite nanosupports and applications thereof in enzyme immobilization: A review

Jiawei Lu et al.

Summary: Enzyme immobilization techniques have evolved significantly, with composite materials playing a crucial role as support materials. Composite nanomaterials, compared to single-component nanomaterials, demonstrate superior performance in enzyme immobilization and other applications. This review aims to provide insights into the design principles of composite nanomaterials for immobilization supports.

COLLOIDS AND SURFACES B-BIOINTERFACES (2022)

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Article Chemistry, Applied

Analysis of film-forming properties of chitosan with different molecular weights and its adhesion properties with different postharvest fruit surfaces

Wanli Zhang et al.

Summary: This study analyzed the film-forming properties and adhesion properties of chitosan films with different molecular weights on fruit surfaces. Higher molecular weight chitosan films showed improved water resistance, water vapor barrier properties, and mechanical properties. The adhesion work of chitosan films with different molecular weights on fruit surfaces was similar, but the 100 and 200 kDa chitosan films had higher adhesion work.

FOOD CHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Gallic acid functionalized chitosan immobilized nanosilver for modified chitosan/Poly (vinyl alcohol) composite film

Yanzhen Zhao et al.

Summary: In this study, silver nanoparticles were reduced and immobilized in situ using gallic acid-functionalized chitosan to achieve a long-lasting and stable controlled release of silver ions. The CG/PVA/AgNPs composite film demonstrated persistent antibacterial effects and improved freshness preservation, showing potential as an effective and safe packaging material to extend the shelf life of food products.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Biochemistry & Molecular Biology

Graphene oxide/chitosan composites as novel support to provide high yield and stable formulations of pectinase for industrial applications

Shagufta Kamal et al.

Summary: In this study, an extracellular pectinase was immobilized onto a graphene oxide/chitosan composite through covalent binding, resulting in enhanced recycling and operational stability features. Optimum conditions were determined, and the immobilized pectinase exhibited higher half-life and retained conformational flexibility compared to the free enzyme. The immobilized pectinase also showed retained catalytic activity after multiple reaction cycles and achieved significant turbidity reduction in guava juice clarification.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Biochemistry & Molecular Biology

Water-soluble amino functionalized chitosan: Preparation, characterization, antioxidant and antibacterial activities

Yuting Yuan et al.

Summary: In this study, a novel water-soluble amino functionalized chitosan was prepared and characterized. The results showed that it exhibited improved water-solubility and excellent antioxidant and antibacterial activities, making it a promising candidate for the development of antioxidant and antibacterial agents.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Materials Science, Multidisciplinary

Highly Stable and Reproducible Biosensor Interface based on Chitosan and 2,5-Di(thienyl)pyrrole based Conjugated Polymer

Betul Cicek Ozkan et al.

Summary: Interface design is crucial for biosensor development, as it directly influences the sensing performance. This study synthesized a new electroactive monomer and polymerized it onto chitosan and chitosan-reduced graphene oxide electrodes to create stable and reproducible sensor platforms. These sensors exhibited higher sensitivity, good selectivity, and long-term stability.

MATERIALS CHEMISTRY AND PHYSICS (2022)

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Review Biochemistry & Molecular Biology

Multifunctional biosensor activities in food technology, microbes and toxins-A systematic mini review

Sugumari Vallinayagam et al.

Summary: Biosensors have significant applications in food safety and health, as they can quickly detect microorganisms and toxins, helping to monitor the quality and freshness of food materials.

PROCESS BIOCHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Fabrication of chitosan-coated magnetite nanobiocatalyst with Bacillus atrophaeus γ-glutamyl transpeptidase and its application to the synthesis of a bioactive peptide SCV-07

Meenu Saini et al.

Summary: Gamma-glutamyl transpeptidase from Bacillus atrophaeus was successfully immobilized onto chitosan-coated magnetite nanoparticles. The immobilized enzyme showed high storage stability and conversion rate, indicating its potential for sustainable synthesis of gamma-glutamyl compounds.

PROCESS BIOCHEMISTRY (2022)

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Review Chemistry, Applied

A review on chitosan-gelatin nanocomposites: Synthesis, characterization and biomedical applications

Sapna Sethi et al.

Summary: Chitosan-gelatin nanocomposites are emerging rapidly as a wide range of materials with diverse properties for biomedical applications. They provide a biocompatible and bioactive base, and can improve the mechanical strength and drug release capacity of the resulting material.

REACTIVE & FUNCTIONAL POLYMERS (2022)

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Article Chemistry, Multidisciplinary

The effect of nano-calcium carbonate on fl-glucosidase immobilized by alginate and chitosan

Mariam Iqbal et al.

Summary: Nanotechnology has been applied to the immobilization process of enzymes, and it has been found that using nano-calcium carbonate as a carrier can improve the stability and reusability of fl-glucosidase. By coating nano-calcium carbonate with natural polymer sodium alginate and adsorbing chitosan, and crosslinking with genipin, the immobilized enzyme has high recovery efficiency and immobilization yield, and shows improved performance under different conditions.

GREEN SYNTHESIS AND CATALYSIS (2022)

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Article Chemistry, Applied

Novel chitosan based metal-organic polyhedrons/enzyme hybrid hydrogel with antibacterial activity to promote wound healing

Jie Song et al.

Summary: This study aims to develop a novel chitosan based metal-organic polyhedrons (MOPs)/enzyme hybrid hydrogel with superior antimicrobial properties for wound healing treatment. The results show that the hydrogel exhibits better bactericidal effect and good cell compatibility, effectively facilitating wound healing. Therefore, the chitosan based MOPs/enzyme hybrid hydrogel holds great promise for antibacterial therapy in clinical applications.

CARBOHYDRATE POLYMERS (2022)

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Article Chemistry, Physical

Electrochemical properties of enzyme electrode covalently immobilized on a graphite oxide/cobalt hydroxide/chitosan composite mediator for biofuel cells

Ji Hyun Yang et al.

Summary: The study aimed to enhance power output by optimizing electrical communication for GOx on a composite surface and found that immobilized GOx at a concentration of 40:80 mM EDC:NHS exhibited the highest activity with a power density of 2.24 mWcm(-2) under optimal conditions.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY (2021)

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Article Chemistry, Applied

Review of chitosan composite as a heavy metal adsorbent: Material preparation and properties

Shabbah Begum et al.

Summary: The focus of the research is on the application of chitosan and its derivatives in removing heavy metals from water bodies, exploring different modification strategies and specific application methods.

CARBOHYDRATE POLYMERS (2021)

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Review Chemistry, Applied

Chitosan based adsorbents for the removal of phosphate and nitrate: A critical review

S. Abdelazeem Eltaweil et al.

Summary: The industrial sector's development leads to harmful effluent discharge, impacting aquatic systems. Chitosan as an adsorbent holds promise for contaminant removal but faces limitations. Research on modified chitosan for phosphate and nitrate removal is ongoing, with a focus on enhancing adsorption characteristics and addressing reusability issues.

CARBOHYDRATE POLYMERS (2021)

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Article Chemistry, Applied

Limited hydrolysis and conjugation of zein with chitosan oligosaccharide by enzymatic reaction to improve functional properties

Wanying He et al.

Summary: By partially hydrolyzing zein with trypsin and conjugating it to chitosan oligosaccharide lactate with transglutaminase, its aqueous solubility and emulsifying function can be significantly improved. Enzymatic glycosylation of chitosan oligosaccharide under optimized conditions shows great potential in enhancing functional properties.

FOOD CHEMISTRY (2021)

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Review Biochemical Research Methods

Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry

Gregory A. Ellis et al.

Summary: This article discusses the method of immobilizing enzymes onto nanoparticles to improve their industrial applicability, with a focus on the analytical methods used and insights into the phenomena that give rise to this enhancement.

CURRENT OPINION IN BIOTECHNOLOGY (2021)

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Article Engineering, Environmental

Co-immobilization of laccase and ABTS onto amino-functionalized ionic liquid-modified magnetic chitosan nanoparticles for pollutants removal

Xiang Qiu et al.

Summary: This study aimed to achieve the co-immobilization of laccase and electron mediator ABTS for improved catalytic performance in removing pollutants. The novel biocatalyst showed high immobilization capacity, enhanced activity, and good removal efficiency for various pollutants in water, indicating its potential practical application value.

JOURNAL OF HAZARDOUS MATERIALS (2021)

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Article Biotechnology & Applied Microbiology

Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance

Hongyi Tu et al.

Summary: A novel magnetic thermosensitive polymer composite carrier with target spacing was developed to enhance the performance of immobilized penicillin G acylase (PGA). Fe3O4 nanoparticles were synthesized and modified using a silane coupling agent and then grafted with a thermosensitive polymer. The immobilized PGA showed improved catalytic activity and recovery rate, making it a promising candidate for biocatalysis applications.

ENZYME AND MICROBIAL TECHNOLOGY (2021)

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Article Chemistry, Applied

High performance biocatalyst based on β-D-galactosidase immobilized on mesoporous silica/titania/chitosan material

Natalia Carminatti Ricardi et al.

Summary: A new support consisting of mesoporous silica/titania with a chitosan coating was developed for immobilizing /9-D-galactosidase from Kluyveromyces lactis. The support showed high immobilization efficiency, maintained enzymatic activity, and exhibited excellent stability in both continuous flow and batch systems, making it suitable for food manufacturing applications.

FOOD CHEMISTRY (2021)

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Article Chemistry, Applied

An enzymatic histamine biosensor based on a screen-printed carbon electrode modified with a chitosan-gold nanoparticles composite cryogel on Prussian blue-coated multi-walled carbon nanotubes

Natha Nontipichet et al.

Summary: A histamine biosensor was developed using a Prussian blue-modified carbon electrode for detecting histamine levels in fish and shrimps. The biosensor demonstrated good specificity, high operational stability, and excellent long-term stability. It successfully measured different levels of histamine through two linear ranges.

FOOD CHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

Production of lactobionic acid using an immobilized cellobiose dehydrogenase/laccase system on magnetic chitosan spheres

Junhua Yang et al.

Summary: Lactobionic acid (LBA) has promising applications in various fields and can be efficiently produced using a new cellobiose dehydrogenase. The immobilized AfCDH/LAC system demonstrates high efficiency and reusability in LBA production, making it a viable option for industrial implementation.

PROCESS BIOCHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

Immobilization of Trametes versicolor laccase on chitosan/halloysite as a biocatalyst in the Remazol Red RR dye

Ruya Hurmuzlu et al.

Summary: The study demonstrated that immobilized laccase on CTS/HNT beads can achieve efficient dye removal and has good reusability. Different parameters and mediators have significant effects on laccase activity and dye removal efficiency.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Article Biochemistry & Molecular Biology

Fabrication of stable electrospun blended chitosan-poly(vinyl alcohol) nanofibers for designing naked-eye colorimetric glucose biosensor based on GOx/HRP

Bilge Coskuner Filiz et al.

Summary: This study successfully designed a stable CS/PVA nanofiber structure for naked-eye colorimetric glucose detection using cost-effective electrospinning technology. The nanofibers exhibited good stability and readability, showing linear glucose analysis response in experiments and achieving significant color changes.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Article Biotechnology & Applied Microbiology

Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads

Roberto Morellon-Sterling et al.

Summary: Enzymes such as trypsin, chymotrypsin, penicillin G acylase, and ficin extract were stabilized through immobilization on glyoxyl agarose with the addition of different aliphatic compounds containing a primary amine group. The presence of amines was found to decrease the enzyme-support attachment covalently and reduce enzyme stability, with octyl amine having the most significant effect. The steric hindrance caused by aminated compounds interacting with aldehyde groups on the enzyme/support multi-reaction plays a key role in decreasing enzyme stability, even with just 1 mM of aminated compounds.

JOURNAL OF BIOTECHNOLOGY (2021)

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Review Chemistry, Multidisciplinary

Chitosan-based membranes preparation and applications: Challenges and opportunities

Shivani S. Vedula et al.

Summary: Chitosan, as an abundant natural biopolymer, is widely used in pharmacology, wastewater treatment, biomedical engineering, and other fields. Through modification and derivatization, chitosan can be transformed into various forms such as membranes, sponges, and fibers. The degradable membranes made from chitosan in conjunction with other materials have diverse applications in areas such as adsorption, medicine, and catalysis.

JOURNAL OF THE INDIAN CHEMICAL SOCIETY (2021)

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Review Chemistry, Applied

Employment of polysaccharides in enzyme immobilization

Archita Sharma et al.

Summary: Immobilized enzymes, with their sturdy and resistant characteristics, have high catalytic activity and show potential for practical applications. The exploitation of polysaccharides as enzyme supports has gained attention for its versatility and wide application in various industries.

REACTIVE & FUNCTIONAL POLYMERS (2021)

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Review Environmental Sciences

Highly effective enzymes immobilization on ceramics: Requirements for supports and enzymes

Joanna Kujawa et al.

Summary: The article summarizes the methods and key elements of enzyme immobilization, including the selection and properties of supports, the impact of modifiers and crosslinkers on enzymes, and the functional groups of immobilized enzymes. By optimizing these factors, efficient immobilization of enzymes and good stability can be achieved.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

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Article Environmental Sciences

Electrochemical Sensor Based on Reduced Graphene Oxide/Double-Walled Carbon Nanotubes/Octahedral Fe3O4/Chitosan Composite for Glyphosate Detection

Cao Thi Thanh et al.

Summary: A novel method for detecting glyphosate using composite material modified electrodes was developed, showing high accuracy and recovery rates in detecting glyphosate in river water. The method has great potential for environmental monitoring applications.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY (2021)

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Article Chemistry, Applied

Intermolecular interactions of chitosan: Degree of acetylation and molecular weight

Chanoong Lim et al.

Summary: The degree of acetylation (DA) of chitosan is crucial for determining its physical, chemical, and biological properties. This study investigated the molecular interactions of chitosan thin films under different molecular weight and DA conditions. Results showed that the interactions of low molecular weight and high molecular weight chitosan were influenced by different factors.

CARBOHYDRATE POLYMERS (2021)

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Article Chemistry, Applied

Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials

Yixin Xu et al.

Summary: The research focused on constructing biodegradable films using chitosan, bacterial cellulose, and curcumin. Results showed that chitosan with higher molecular weight exhibited superior properties and released active substances when placed in 95% ethanol, indicating potential use as promising materials for food packaging.

CARBOHYDRATE POLYMERS (2021)

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Review Biochemistry & Molecular Biology

Different strategies for the lipase immobilization on the chitosan based supports and their applications

F. Rafiee et al.

Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Review Biochemistry & Molecular Biology

The bonding nature of the chemical interaction between trypsin and chitosan based carriers in immobilization process depend on entrapped method: A review

Shamo Zokhrab Tapdigov

Summary: The review article focuses on the chemical bond formed during the immobilization of the proteolytic enzyme pancreatic trypsin in chitosan-based polymer matrixes and its derivatives. By studying the chemical nature of functional groups, the degree of ionization, and other factors, researchers found that the mechanism of trypsin immobilization is affected, resulting in attraction between the enzyme and the matrix due to various forces such as polar covalent bonds and hydrogen bonds.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Review Engineering, Environmental

Chitosan modifications for adsorption of pollutants-A review

Ismaila Olalekan Saheed et al.

Summary: Modification of chitosan through methods such as grafting and cross-linking is a promising approach for removing organic and inorganic pollutants from aqueous medium. The performance of modified chitosan for adsorption of pollutants depends largely on the modification method adopted, materials used, and adsorption experimental conditions. Cross-linking is commonly used for improving the stability of chitosan, although it may decrease adsorption capacity, but recent works have shown enhancement through grafting or incorporation of solid adsorbents before cross-linking.

JOURNAL OF HAZARDOUS MATERIALS (2021)

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Article Materials Science, Multidisciplinary

Electrospun chitosan coated polylactic acid nanofiber: A novel immobilization matrix for α -Amylase and its application in hydrolysis of cassava fibrous waste

Kiran Kumar Gali et al.

Summary: This study explores the potential of using Cassava fibrous waste for production of fermentable sugars through enzymatic hydrolysis, with the use of tailor-made electrospun nanofibers for immobilization of alpha-amylase to overcome processing issues. The chitosan coated nano-fiber matrix showed enhanced performance in immobilizing the enzyme, resulting in improved conversion ratios at optimized conditions.

JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T (2021)

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Article Biotechnology & Applied Microbiology

High specific immobilization of His-tagged recombinant Microbacterium esterase by Ni-NTA magnetic chitosan microspheres for efficient synthesis of key chiral intermediate of d-biotin

Song He et al.

Summary: The novel Ni-NTA-functionalized magnetic chitosan microspheres (MCS-NTA-Ni) were prepared, providing high enzyme loading and selective adsorption of his-tagged recombinant protein. Immobilized esterase showed higher pH stability, thermal stability, and excellent reusability for substrate conversion.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2021)

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Review Chemistry, Applied

Current trends and challenges in the synthesis and applications of chitosan-based nanocomposites for plants: A review

Juntong Yu et al.

Summary: Chitosan, a low-cost and versatile polymer, has been extensively used in agriculture, pharmacy, and biomedicine industries. Chitosan-based nanocomposites can enhance efficiency and widen application areas in plants.

CARBOHYDRATE POLYMERS (2021)

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Review Chemistry, Applied

Laccase immobilization onto natural polysaccharides for biosensing and biodegradation

Zahra Shokri et al.

Summary: This review paper discusses the significance and potential of using natural polysaccharides as support materials for enzyme immobilization, especially laccases, to improve their stability and selectivity in industrial applications. Additionally, the roles of immobilized laccases are examined from a fundamental perspective to elucidate their catalytic mechanisms in detecting and degrading environmental contaminants.

CARBOHYDRATE POLYMERS (2021)

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Review Biochemistry & Molecular Biology

Chitosan-based nanofibers for enzyme immobilization

Eduardo Silveira Ribeiro et al.

Summary: Chitosan-based nanofibers exhibit outstanding properties for enzyme immobilization, enhancing stability and efficiency. Different methods of enzyme immobilization, such as adsorption, cross-linking, and entrapment, each have their own advantages and challenges that need to be addressed. The application of chitosan-based nanofibers in enzyme immobilization offers opportunities to improve catalytic activity and extend the lifetime of enzymes.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Article Biotechnology & Applied Microbiology

Design of bioreactor based on immobilized laccase on silica-chitosan support for phenol removal in continuous mode

A. M. Girelli et al.

Summary: The immobilization of laccase on a silica-chitosan support using calcium ion as a linking agent resulted in efficient phenol removal in a flow system. The immobilized enzyme reactor showed good storage stability and reusability. The optimal phenol removal performance was achieved with low molecular weight chitosan from crab shells at pH 5 and a flow rate of 0.7 mL/min.

JOURNAL OF BIOTECHNOLOGY (2021)

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Article Food Science & Technology

Chitosan derivative functionalized carbon nanotubes as carriers for enzyme immobilization to improve synthetic efficiency of ethyl caproate

Suping Ji et al.

Summary: The study successfully utilized CA-CS conjugates to modify carbon nanotubes, enabling the immobilization of porcine pancreatic lipase on the nanotubes with good dispersion and biocompatibility. The resulting CA-CS-CNTs-PPL biocatalyst exhibited excellent catalytic performance.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

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Article Engineering, Environmental

Ofloxacin exclusion using novel HRP immobilized chitosan cross-link with graphene-oxide nanocomposite

Akansha Suri et al.

Summary: The green nano adsorbent HRP-CsGOn, prepared by cross-linking chitosan with graphene oxide and immobilizing horseradish peroxidase onto the material, showed effective adsorption of Ofloxacin and demonstrated good temperature, pH stability, and lifespan. Additionally, the adsorbent exhibited excellent reusability, making it a promising material for various applications.

GROUNDWATER FOR SUSTAINABLE DEVELOPMENT (2021)

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Article Chemistry, Multidisciplinary

Improvement of power generation of enzyme fuel cell by novel GO/Co/chitosan electrodeposition

Dong Sup Kim et al.

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY (2020)

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Review Chemistry, Multidisciplinary

Chitin and chitosan-based support materials for enzyme immobilization and biotechnological applications

Madan L. Verma et al.

ENVIRONMENTAL CHEMISTRY LETTERS (2020)

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Article Biochemistry & Molecular Biology

Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces

C. Salvo-Comino et al.

BIOELECTROCHEMISTRY (2020)

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Article Chemistry, Applied

Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Aurileide M. B. F. Soares et al.

CARBOHYDRATE POLYMERS (2020)

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Article Biochemistry & Molecular Biology

Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes

Ahmet Ulu et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Biochemistry & Molecular Biology

Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds

Han Wang et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Chemistry, Analytical

Uric acid enzyme biosensor based on a screen-printed electrode coated with Prussian blue and modified with chitosan-graphene composite cryogel

Ratchaneekorn Jirakunakorn et al.

MICROCHEMICAL JOURNAL (2020)

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Article Biochemistry & Molecular Biology

Recombinant Horseradish Peroxidase C1A Immobilized on Hydrogel Matrix for Dye Decolorization and Its Mechanism on Acid Blue 129 Decolorization

Ya-Jing Wang et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2020)

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Article Biotechnology & Applied Microbiology

Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde

Haodao Mo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

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Article Chemistry, Applied

Effect of eggshell membrane as porogen on the physicochemical structure and protease immobilization of chitosan-based macroparticles

Yuanyuan Liu et al.

CARBOHYDRATE POLYMERS (2020)

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Review Biochemistry & Molecular Biology

Carbohydrate and protein based biopolymeric nanoparticles: Current status and biotechnological applications

Madan L. Verma et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Biotechnology & Applied Microbiology

Silica-chitosan hybrid support for laccase immobilization

A. M. Girelli et al.

JOURNAL OF BIOTECHNOLOGY (2020)

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Article Materials Science, Multidisciplinary

Immobilization of laccase on chitosan-clay composite beads to improve its catalytic efficiency to degrade industrial dyes

Seema Mehandia et al.

MATERIALS TODAY COMMUNICATIONS (2020)

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Article Biochemistry & Molecular Biology

Fabrication of an organic-inorganic nanocomposite carrier for enzyme immobilization based on metal-organic coordination

Xiang Qiu et al.

PROCESS BIOCHEMISTRY (2020)

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Review Chemistry, Applied

On the taught new tricks of enzymes immobilization: An all-inclusive overview

Roswanira Abdul Wahab et al.

REACTIVE & FUNCTIONAL POLYMERS (2020)

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Article Chemistry, Applied

Effect of deacetylation degree of chitosan on rheological properties and physical chemical characteristics of genipin-crosslinked chitosan beads

Loleny Tavares et al.

FOOD HYDROCOLLOIDS (2020)

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Article Multidisciplinary Sciences

Applications of chitosan (CHI)-reduced graphene oxide (rGO)-polyaniline (PAni) conducting composite electrode for energy generation in glucose biofuel cell

Sufia ul Haque et al.

SCIENTIFIC REPORTS (2020)

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Article Biochemistry & Molecular Biology

The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal

Jakub Zdarta et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Engineering, Chemical

Development of Co-hemin MOF/chitosan composite based biosensor for rapid detection of lactose

Han Suk Choi et al.

JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS (2020)

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Article Materials Science, Multidisciplinary

Novel enzymatically crosslinked chitosan hydrogels with free-radical-scavenging property and promoted cellular behaviors under hyperglycemia

Dieu Linh Tran et al.

PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL (2020)

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Article Biochemistry & Molecular Biology

Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study

Thitinun Karpkird et al.

CARBOHYDRATE RESEARCH (2020)

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Article Chemistry, Physical

Synthesis and characterization of a novel magnetic chitosan microsphere for lactase immobilization

Ping Ke et al.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2020)

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Article Chemistry, Multidisciplinary

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Avinash A. Kadam et al.

NANOMATERIALS (2020)

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Article Food Science & Technology

Modulating properties of polysaccharides nanocomplexes from enzymatic hydrolysis of chitosan

Larissa Ribas Fonseca et al.

FOOD RESEARCH INTERNATIONAL (2020)

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Article Chemistry, Physical

Laccase immobilized on chitosan-coated Fe3O4 nanoparticles as reusable biocatalyst for degradation of chlorophenol

Kai Zhang et al.

JOURNAL OF MOLECULAR STRUCTURE (2020)

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Article Chemistry, Applied

Potential of chitosan-based membranes for the separation of essential oil components by target-organophilic pervaporation

Wendel P. Silvestre et al.

CARBOHYDRATE POLYMERS (2020)

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Article Agricultural Engineering

Conversion of biogas to methanol by methanotrophs immobilized on chemically modified chitosan

Sanjay K. S. Patel et al.

BIORESOURCE TECHNOLOGY (2020)

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Article Biotechnology & Applied Microbiology

Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads

Liu Yang et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2019)

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Article Biochemistry & Molecular Biology

Development of Voltammetric Glucose-6-phosphate Biosensors Based on the Immobilization of Glucose-6-phosphate Dehydrogenase on Polypyrrole- and Chitosan-Coated Fe3O4 Nanoparticles/Polypyrrole Nanocomposite Films

Selmihan Sahin et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2019)

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Article Biotechnology & Applied Microbiology

Lipase-immobilized chitosan-crosslinked magnetic nanoparticle as a biocatalyst for ring opening esterification of itaconic anhydride

Seyed Mehdi Hosseini et al.

BIOCHEMICAL ENGINEERING JOURNAL (2019)

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Review Biochemistry & Molecular Biology

Chitosan-functionalized nanofibers: A comprehensive review on challenge's and prospects for food applications

Bruna Silva de Farias et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Article Biochemistry & Molecular Biology

Agarose-chitosan hydrogel-immobilized horseradish peroxidase with sustainable bio-catalytic and dye degradation properties

Muhammad Bilal et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Article Biochemistry & Molecular Biology

Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite

Mitra Naghdi et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Article Materials Science, Biomaterials

Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization

Hongbo Suo et al.

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2019)

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Article Chemistry, Applied

Chitosan Co-polymeric nanostructures for catalase immobilization

T. Inanan

REACTIVE & FUNCTIONAL POLYMERS (2019)

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Review Biochemistry & Molecular Biology

Chitosan oligosaccharide (COS): An overview

Muhammad Naveed et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Article Biochemistry & Molecular Biology

Immobilization of β-galactosidase on chitosan-coated magnetic nanoparticles and its application for synthesis of lactulose-based galactooligosaccharides

Vuong D. Nguyen et al.

PROCESS BIOCHEMISTRY (2019)

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Article Green & Sustainable Science & Technology

Synthesis of chitosan-cellulase nanohybrid and immobilization on alginate beads for hydrolysis of ionic liquid pretreated sugarcane bagasse

Koel Saha et al.

RENEWABLE ENERGY (2019)

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Article Biochemistry & Molecular Biology

Chitosan activated with divinyl sulfone: a new heterofunctional support for enzyme immobilization. Application in the immobilization of lipase B from Candida antarctica

Bruna B. Pinheiro et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Review Biochemistry & Molecular Biology

Naturally-derived biopolymers: Potential platforms for enzyme immobilization

Muhammad Bilal et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

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Article Materials Science, Multidisciplinary

Clay/chitosan biocomposite systems as novel green carriers for covalent immobilization of food enzymes

Ilaria Cacciotti et al.

JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T (2019)

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Article Biophysics

An acetylcholinesterase biosensor based on doping Au nanorod@SiO2 nanoparticles into TiO2-chitosan hydrogel for detection of organophosphate pesticides

Hui-Fang Cui et al.

BIOSENSORS & BIOELECTRONICS (2019)

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Review Chemistry, Applied

A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production

Xubing Yuan et al.

CARBOHYDRATE POLYMERS (2019)

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Article Food Science & Technology

Production of low molecular weight chitosan by acid and oxidative pathways: Effect on physicochemical properties

Bruna Silva de Farias et al.

FOOD RESEARCH INTERNATIONAL (2019)

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Article Engineering, Environmental

Biodegradation of some azo dyes from wastewater with laccase from Weissella viridescens LB37 immobilized on magnetic chitosan nanoparticles

Hayrunnisa Nadaroglu et al.

JOURNAL OF WATER PROCESS ENGINEERING (2019)

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Article Chemistry, Multidisciplinary

Facile and green fabrication of biocatalytic chitosan beads by one-step genipin-mediated β-glucosidase immobilization for production of bioactive genistein

Noppharat Phadungcharoen et al.

SUSTAINABLE CHEMISTRY AND PHARMACY (2019)

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Article Biotechnology & Applied Microbiology

Covalent immobilization of soybean seed hull urease on chitosan mini-spheres and the impact on their properties

Lautaro Fidel Bracco et al.

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY (2019)

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Article Multidisciplinary Sciences

Ternary Blended Chitosan/Chitin/FE3O4 Nanosupport for Lipase Activation and Stabilization

Ida Nurhazwani Abdul Rahman et al.

ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING (2019)

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Article Environmental Sciences

Laccase Validation as Pretreatment of Agave Waste Prior to Saccharification: Free and Immobilized in Superparamagnetic Nanoparticles Enzyme Preparations

Jaquelina Sanchez-Ramirez et al.

WASTE AND BIOMASS VALORIZATION (2018)

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Article Biochemistry & Molecular Biology

Immobilization of Carboxypeptidase A into Modified Chitosan Matrixes by Covalent Attachment

Ricardo M. Manzo et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2018)

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Article Chemistry, Physical

Immobilization of Rhus vernicifera laccase on sepiolite; effect of chitosan and copper modification on laccase adsorption and activity

Yaniv Olshansky et al.

APPLIED CLAY SCIENCE (2018)

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Article Chemistry, Applied

The role played by the molecular weight and acetylation degree in modulating the stiffness and elasticity of chitosan gels

Pasquale Sacco et al.

CARBOHYDRATE POLYMERS (2018)

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Review Chemistry, Applied

Antioxidant activities of chitosans and its derivatives in in vitro and in vivo studies

Makoto Anraku et al.

CARBOHYDRATE POLYMERS (2018)

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Article Biophysics

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent

Xinran Xiang et al.

COLLOIDS AND SURFACES B-BIOINTERFACES (2018)

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Article Biophysics

Preparation and characterization of a novel nanocomposite with double enzymes immobilized on magnetic Fe3O4-chitosan-sodium tripolyphosphate

Zhongqin Chen et al.

COLLOIDS AND SURFACES B-BIOINTERFACES (2018)

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Article Chemistry, Applied

Effective production of resistant starch using pullulanase immobilized onto magnetic chitosan/Fe3O4 nanoparticles

Jie Long et al.

FOOD CHEMISTRY (2018)

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Article Chemistry, Applied

Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds

Rafael D. S. Azevedo et al.

FOOD CHEMISTRY (2018)

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Article Chemistry, Applied

Highly stable novel silica/chitosan support for beta-galactosidase immobilization for application in dairy technology

Natalia Carminatti Ricardi et al.

FOOD CHEMISTRY (2018)

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Article Biochemistry & Molecular Biology

Tannase immobilisation by amino-functionalised magnetic Fe3O4-chitosan nanoparticles and its application in tea infusion

Ruyi Li et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

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Article Polymer Science

Preparation and Evaluation of Coal Fly Ash/Chitosan Composites as Magnetic Supports for Highly Efficient Cellulase Immobilization and Cellulose Bioconversion

Limin Zang et al.

POLYMERS (2018)

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Article Chemistry, Applied

Affecting parameters on fabrication of beta-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers

Sara Haghju et al.

CARBOHYDRATE POLYMERS (2018)

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Article Biochemistry & Molecular Biology

Chitosan-alginate immobilized lipase based catalytic constructs: Development, characterization and potential applications

Robina Rashid et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

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Article Chemistry, Analytical

Amperometric Glucose Biosensor Utilizing Zinc Oxide-chitosan-glucose Oxidase Hybrid Composite Films on Electrodeposited Pt-Fe(III)

Eun-Sook Paik et al.

ANALYTICAL SCIENCES (2018)

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Review Chemistry, Multidisciplinary

Nanobiotechnology advances in enzymatic biosensors for the agri-food industry

Madan L. Verma

ENVIRONMENTAL CHEMISTRY LETTERS (2017)

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Article Biochemistry & Molecular Biology

α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening

Dong-Mei Liu et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)

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Article Biochemistry & Molecular Biology

Improved catalytic properties of Penicillium notatum lipase immobilized in nanoscale silicone polymeric films

Saimaa Rehman et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)

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Article Biochemistry & Molecular Biology

Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst

Ali Baghban et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)

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Article Food Science & Technology

Delignification and fruit juice clarification properties of alginate-chitosan-immobilized ligninolytic cocktail

Muhammad Bilal et al.

LWT-FOOD SCIENCE AND TECHNOLOGY (2017)

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Article Biochemistry & Molecular Biology

Physical crosslinking of lipase from Rhizomucor miehei immobilized on octyl agarose via coating with ionic polymers Avoiding enzyme release from the support

Laura Fernandez-Lopez et al.

PROCESS BIOCHEMISTRY (2017)

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Article Chemistry, Applied

Chitosan crosslinked with genipin as support matrix for application in food process: Support characterization and β-D-galactosidase immobilization

Manuela P. Klein et al.

CARBOHYDRATE POLYMERS (2016)

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Article Chemistry, Applied

Chemical amination of lipases improves their immobilization on octyl-glyoxyl agarose beads

Nazzoly Rueda et al.

CATALYSIS TODAY (2016)

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Article Chemistry, Applied

Effect of chitosan molecular weight on the formation of chitosan-pullulanase soluble complexes and their application in the immobilization of pullulanase onto Fe3O4-κ-carrageenan nanoparticles

Jie Long et al.

FOOD CHEMISTRY (2016)

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Article Biochemistry & Molecular Biology

Characterization of β-glucosidase immobilized on chitosan-multiwalled carbon nanotubes (MWCNTS) and their application on tea extracts for aroma enhancement

Akile Celik et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2016)

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Article Biochemistry & Molecular Biology

Enantioselective acetylation of (R,S)-atenolol: The use of Candida rugosa lipases immobilized onto magnetic chitosan nanoparticles in enzyme-catalyzed biotransformation

Adam Sikora et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2016)

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Article Biochemistry & Molecular Biology

Enhancing catalytic performance of laccase via immobilization on chitosan/CeO2 microspheres

Jiahong Lin et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2015)

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Article Biochemistry & Molecular Biology

Immobilization of Pepsin on Chitosan Magnetic Nanoparticles and Its Application in Deacetylation of Amides

Moslem Mansour Lakouraj et al.

JOURNAL OF CARBOHYDRATE CHEMISTRY (2015)

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Article Biophysics

An amperometric β-glucan biosensor based on the immobilization of bi-enzyme on Prussian blue-chitosan and gold nanoparticles-chitosan nanocomposite films

Beibei Wang et al.

BIOSENSORS & BIOELECTRONICS (2014)

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Article Chemistry, Analytical

Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system

Dipali R. Bagal-Kestwal et al.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2014)

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Article Chemistry, Analytical

Construction and application of an amperometric uric acid biosensor based on covalent immobilization of uricase on iron oxide nanoparticles/chitosan-g-polyaniline composite film electrodeposited on Pt electrode

Rooma Devi et al.

SENSORS AND ACTUATORS B-CHEMICAL (2014)

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Article Biophysics

An amperometric glutamate biosensor based on immobilization of glutamate oxidase onto carboxylated multiwalled carbon nanotubes/gold nanoparticles/chitosan composite film modified Au electrode

Bhawna Batra et al.

BIOSENSORS & BIOELECTRONICS (2013)

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Article Chemistry, Applied

Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization

Kristyna Pospiskova et al.

CARBOHYDRATE POLYMERS (2013)

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Article Engineering, Environmental

Chitosan-halloysite hybrid-nanotubes: Horseradish peroxidase immobilization and applications in phenol removal

Rui Zhai et al.

CHEMICAL ENGINEERING JOURNAL (2013)

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Article Biotechnology & Applied Microbiology

Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors

Heru Susanto et al.

ENZYME AND MICROBIAL TECHNOLOGY (2013)

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Article Biochemistry & Molecular Biology

Immobilization of lysozyme-CLEA onto electrospun chitosan nanofiber for effective antibacterial applications

Jae-Min Park et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2013)

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Article Engineering, Biomedical

Positive charge of chitosan retards blood coagulation on chitosan films

Qing He et al.

JOURNAL OF BIOMATERIALS APPLICATIONS (2013)

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Article Chemistry, Applied

Optimum conditions for lipase immobilization on chitosan-coated Fe3O4 nanoparticles

Chia-Hung Kuo et al.

CARBOHYDRATE POLYMERS (2012)

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Article Chemistry, Analytical

Immobilization of laccase in N-doped carbon hollow spheres/chitosan composite film for electrochemical detection of kraft lignin

Ying Li et al.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY (2012)

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Article Chemistry, Analytical

Potentiometric glucose sensor based on the glucose oxidase immobilized iron ferrite magnetic particle/chitosan composite modified gold coated glass electrode

K. Khun et al.

SENSORS AND ACTUATORS B-CHEMICAL (2012)

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Article Chemistry, Physical

Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized on bamboo shaped carbon nanotubes/chitosan matrix

Zanzan Zhu et al.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2011)

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Article Chemistry, Analytical

Development of dip-strip sucrose sensors: Application of plant invertase immobilized in chitosan-guar gum, gelatin and poly-acrylamide films

Dipali Bagal-Kestwal et al.

SENSORS AND ACTUATORS B-CHEMICAL (2011)

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Article Biophysics

Biocompatible electrochemiluminescent biosensor for choline based on enzyme/titanate nanotubes/chitosan composite modified electrode

Hong Dai et al.

BIOSENSORS & BIOELECTRONICS (2010)

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Article Materials Science, Multidisciplinary

Surface functionalization of chitosan-coated magnetic nanoparticles for covalent immobilization of yeast alcohol dehydrogenase from Saccharomyces cerevisiae

Gui-yin Li et al.

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS (2010)

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Article Materials Science, Biomaterials

Development of amperometric laccase biosensor through immobilizing enzyme in copper-containing ordered mesoporous carbon (Cu-OMC)/chitosan matrix

Xinhua Xu et al.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2010)

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Article Biochemistry & Molecular Biology

Direct electrochemistry of glucose oxidase immobilized on NdPO4 nanoparticles/chitosan composite film on glassy carbon electrodes and its biosensing application

Qinglin Sheng et al.

BIOELECTROCHEMISTRY (2009)

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Article Chemistry, Applied

Effects of degree of deacetylation on enzyme immobilization in hydrophobically modified chitosan

Kyle H. Sjoholm et al.

CARBOHYDRATE POLYMERS (2009)

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Article Electrochemistry

One-step fabrication of three-dimensional porous calcium carbonate-chitosan composite film as the immobilization matrix of acetylcholinesterase and its biosensing on pesticide

Jingming Gong et al.

ELECTROCHEMISTRY COMMUNICATIONS (2009)

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Article Biochemistry & Molecular Biology

Amperometric hydrogen peroxide biosensor based on the immobilization of HRP on DNA-silver nanohybrids and PDDA-protected gold nanoparticles

Liping Ma et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2009)

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Article Chemistry, Analytical

Application of functionalised carbon nanotubes immobilised into chitosan films in amperometric enzyme biosensors

Mariana Emilia Ghica et al.

SENSORS AND ACTUATORS B-CHEMICAL (2009)

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Article Biotechnology & Applied Microbiology

Use of chitosan-clay composite as immobilization support for improved activity and stability of β-glucosidase

Min-Yun Chang et al.

BIOCHEMICAL ENGINEERING JOURNAL (2007)

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Article Chemistry, Analytical

Electrogenerated chemiluminescence biosensor based on Ru(bpy)32+ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material

Lihua Zhang et al.

ANALYTICA CHIMICA ACTA (2006)

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Review Biotechnology & Applied Microbiology

Application of chitin- and chitosan-based materials for enzyme immobilizations: a review

B Krajewska

ENZYME AND MICROBIAL TECHNOLOGY (2004)

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