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Selective inhibitory effects of suberosin on CYP1A2 in human liver microsomes

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Identification of suberosin metabolites in human liver microsomes by high-performance liquid chromatography combined with high-resolution quadrupole-orbitrap mass spectrometer

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Summary: Suberosin, a natural prenylated coumarin derivative, has been found to have various pharmacological properties, particularly as an anticoagulant. This study characterized the metabolic pathway and metabolites of suberosin in human liver microsomes, identifying eight metabolites and determining CYP1A2 and CYP2B6 as the major enzymes involved in its metabolism. Hydroxylation was found to be the predominant metabolic pathway in the phase I metabolism of suberosin. Further research is needed to evaluate the efficacy and toxicity of these metabolites in an in vivo system.

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