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Anticancer activity of thymoquinone against breast cancer cells: Mechanisms of action and delivery approaches

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Nida Alsaffar et al.

Summary: An increasing body of literature demonstrates the therapeutic relevance of polyphenols. Thymoquinone, a major bioactive polyphenol constituent, affects the glutathione S-transferases (GST) and cell cycle in eukaryotic cells. The inhibition of cell proliferation, altered enzyme activity and isozyme expression, and reduced morphogenesis and fruiting body formation were observed in the eukaryotic model Dictyostelium discoideum under the influence of thymoquinone.

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Thymoquinone, piperine, and sorafenib combinations attenuate liver and breast cancers progression: epigenetic and molecular docking approaches

Ashraf A. El-Shehawy et al.

Summary: This study found that the combination treatment of TQ and PIP with SOR has potential chemo-modulatory effects against human triple-negative breast cancer and liver cancer cells. The combination treatment enhanced the anti-proliferative and cytotoxic effects of SOR by regulating cell cycle, inducing apoptosis, and modulating the expression of DNMT3B, HDAC3, and miRNA-29c. Molecular docking study identified strong interactions between TQ, PIP, and SOR with DNMT3B and HDAC3, leading to growth arrest and cell death.

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Safety, Stability, and Therapeutic Efficacy of Long-Circulating TQ-Incorporated Liposomes: Implication in the Treatment of Lung Cancer

Arif Khan et al.

Summary: A novel formulation of Thymoquinone (TQ), called PEG-coated DSPC/cholesterol comprising TQ liposomes (PEG-Lip-TQ), has been developed and shown to have high anticancer activity and low toxicity in lung cancer cells. This study suggests that PEG-Lip-TQ could be a potential treatment for lung cancer in clinical settings.

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Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice

Arif Khan et al.

Summary: This study successfully prepared a novel formulation of TQ and evaluated its potential in chemoprevention in a chemically induced lung cancer animal model. The results showed that TQPSL could restore lung organ weight, cancer marker enzyme levels, and serum antioxidant enzyme levels, reduce malignancy in the lungs, induce apoptosis, and decrease intracellular ROS levels. Furthermore, molecular docking results suggested that TQ may have a strong affinity for the BRAF target.

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Zaynab Fatfat et al.

Summary: The long-term success of standard anticancer monotherapeutic strategies has been limited by tolerability issues, treatment resistance, and cancer relapse. Combination therapy has shown promising results in strengthening the anti-tumor potential, reducing toxic effects, and overcoming resistance. Interest in combining natural products with conventional chemotherapeutics to improve treatment efficacy has been growing, with Thymoquinone (TQ) emerging as a promising candidate due to its low toxicity and anticancer efficacy.

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Sultan Alshehri et al.

Summary: The study successfully formulated thymoquinone-encapsulated chitosan-coated nanoparticles using the emulsion evaporation method for improved efficacy against breast cancer. The optimized nanoparticles showed enhanced antioxidant potential and cytotoxicity, demonstrating great potential for breast cancer management.

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Safety and Therapeutic Efficacy of Thymoquinone-Loaded Liposomes against Drug-Sensitive and Drug-Resistant Acinetobacter baumannii

Khaled S. Allemailem et al.

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Thymoquinone, as a Novel Therapeutic Candidate of Cancers

Belal Almajali et al.

Summary: Natural product thymoquinone (TQ) from Nigella sativa has shown promising anticancer activities through inhibiting cell proliferation, migration, and invasion in various human cancers. Its efficacy in inducing apoptosis and mechanisms on different cancer types are still not fully understood, but there is ongoing research on its potential as a future anticancer therapy nominee, especially in combination with other anticancer agents using nanotechnology.

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The role of thymoquinone, a major constituent of Nigella sativa, in the treatment of inflammatory and infectious diseases

Kaneez Fatima Shad et al.

Summary: Nigella sativa and thymoquinone have potential pharmacological properties for a wide range of human diseases. Recent developments in nanoparticulate-based administration may allow the clinical development of these compounds as therapeutic agents. Studies have shown anti-inflammatory and antiviral properties of N. sativa and thymoquinone, making them promising agents for targeting contemporary inflammatory and infectious diseases, including Covid 19.

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Formulation, Characterization and Cytotoxicity Effects of Novel Thymoquinone-PLGA-PF68 Nanoparticles

Nurul Shahfiza Noor et al.

Summary: Thymoquinone has anti-cancer properties but limited clinical application due to its volatile characteristics. A polymeric nanoformulation with PLGA-PEG and Pluronics F68 was developed to conserve thymoquinone's biological activity. The encapsulation showed promising anti-cancer effects against breast cancer cells.

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Thymoquinone-entrapped chitosan-modified nanoparticles: formulation optimization to preclinical bioavailability assessments

Iqra Rahat et al.

Summary: A novel chitosan-modified polycaprolactone nanoparticles (THQ-CPLNPs) were designed and optimized to improve the oral bioavailability of thymoquinone (THQ). The optimized THQ-CPLNPs showed good release and mucoadhesion properties in simulated intestinal fluids. In vivo studies demonstrated a significantly improved oral bioavailability of THQ from THQ-CPLNPs compared to THQ suspension.

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