Mercury Concentration in Saliva and the Impact of Chewing: An Inductively Coupled Plasma Mass Spectrometry Study

Mercury, which is found in dental amalgams, is considered to be the most toxic non-radioactive element. However, the health policies of different countries have not reached a consensus on the use and safety of amalgam. This study aims to investigate the effect of amalgam restorations on mercury concentration in saliva, as well as the effect of restoration number, surface number, and chewing on this concentration. A total of 86 participants were included in this study (an equal number for the study and control groups). The number of amalgam restorations and their surfaces were recorded. While both unstimulated and stimulated saliva were collected from the study group, only unstimulated saliva was collected from the control group. The effect of chewing on mercury concentration was examined in the study group with unstimulated and stimulated saliva specimens using inductively coupled plasma mass spectrometry device. Mercury concentration in the unstimulated saliva was found to be higher in the study group compared to the control group (p= 0.000). As the number of amalgam restorations and the number of amalgam restoration surfaces increased, the mercury concentration in the saliva increased (p= 0.015 and p= 0.021, respectively). There was no statistically significant difference between mercury levels in the unstimulated saliva and the stimulated saliva (p=0.316). Chewing presented an insignificant difference in mercury concentration. Given this surprising result, the effect of chewing on mercury concentrations should be explored more extensively in future research.

Automated summary

This study investigates the impact of amalgam restorations on mercury concentration in saliva, as well as the influence of restoration number, surface number, and chewing. The findings reveal that mercury concentration in unstimulated saliva is higher in the study group compared to the control group, and an increase in the number of amalgam restorations and surfaces leads to higher mercury concentration in saliva. Chewing does not have a significant effect on mercury concentration. Further research should explore the effects of chewing on mercury concentrations.