Two are not enough: synthetic strategies and applications of unnatural base pairs

Article Multidisciplinary Sciences

Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase

Juntaek Oh et al.

Summary: This study reveals the mechanism of how T7 RNA polymerase recognizes unnatural base pairs, providing a theoretical basis for synthesizing RNA molecules with synthetic modifications and unnatural base pairs.

NATURE COMMUNICATIONS (2023)

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Article Chemistry, Multidisciplinary

Copper-Catalyzed Stereoselective Borylation and Palladium-Catalyzed Stereospecific Cross-Coupling to Give Aryl C-Glycosides

Kazuki Kurahayashi et al.

Summary: Metabolically stable C-glycosides play a crucial role in bioactive natural products, therapeutic agents, and biological probes. The development of synthetic methods for connecting glycosides and aglycons with strict stereocontrol is essential. In this study, a copper-catalyzed one-step borylation of glycosyl bromides to glycosyl boronates and a palladium-catalyzed cross-coupling of β-glycosyl borates with aryl bromides were successfully achieved, resulting in the synthesis of aryl β-C-glycosides.

CHEMISTRY-A EUROPEAN JOURNAL (2023)

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Article Chemistry, Multidisciplinary

Chemoselective and Diastereoselective Synthesis of C-Aryl Nucleoside Analogues by Nickel-Catalyzed Cross-Coupling of Furanosyl Acetates with Aryl Iodides

Yuxi Li et al.

Summary: A nickel-catalyzed modular approach is reported for preparing C-aryl nucleoside analogues from readily available compounds. This method features mild reaction conditions, broad substrate scope, excellent selectivity, and high functional group compatibility. The practicality of this transformation is demonstrated through the synthesis of a potent analogue of a naturally occurring NF-kappa B activator.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

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Article Chemistry, Multidisciplinary

Direct and Catalytic C-Glycosylation of Arenes: Expeditious Synthesis of the Remdesivir Nucleoside**

Carla Obradors et al.

Summary: Since early 2020, scientists have been striving to find effective solutions to combat SARS-CoV-2, focusing on developing reliable vaccines and repurposing drugs. However, the current synthetic routes for Remdesivir, the most widely used therapeutic during the early stages of infection, face challenges in cost-effective supply to patients. In this study, researchers have developed a straightforward method for adding (hetero)arenes to unprotected sugars, significantly expediting the synthesis of Remdesivir precursor GS-441524.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

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Article Biophysics

Long-range distance determination in fully deuterated RNA with pulsed EPR spectroscopy

Burkhard Endeward et al.

Summary: Pulsed electron-electron double resonance (PELDOR or DEER) spectroscopy is a powerful method for studying the structure and dynamics of biological macromolecules, providing distance distribution information from 1.8 to 6 nm when the biomolecules are labeled with paramagnetic tags. This study demonstrates the measurement of long-range distances up to 14 nm on a large RNA molecule using a combination of site-directed spin labeling and RNA perdeuteration. The perdeuteration technique extends the observation time windows of PELDOR and allows for the determination of long distances and conformational flexibility in large RNAs.

BIOPHYSICAL JOURNAL (2022)

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Article Chemistry, Multidisciplinary

Halogen-bond-assisted radical activation of glycosyl donors enables mild and stereoconvergent 1,2-cis-glycosylation

Chen Zhang et al.

Summary: The chemistry of carbohydrates has a long history, and a simple, stereoselective and efficient glycosylation method is still needed for studying sugars. This study presents a strategy for 1,2-cis-glycosylation without using metals or strong acids. The method activates glycosyl donors through a radical cascade, resulting in the formation of 1,2-cis-glycosides with high stereoselectivity. The reaction shows remarkable tolerance to functional groups and is applicable to a broad range of donors.

NATURE CHEMISTRY (2022)

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Article Chemistry, Organic

One-Pot Stereoselective Synthesis of 2,3-Diglycosylindoles and Tryptophan-C-glycosides via Palladium-Catalyzed C-H Glycosylation of Indole and Tryptophan

Ya-Nan Ding et al.

Summary: A novel palladium-catalyzed C-H glycosylation method was described for the one-pot stereoselective synthesis of 2,3-diglycosylindoles and tryptophan-C-glycosides. The method offers a convenient and efficient route to construct C-glycosides using air and base-free and ligand-free conditions, and it can be applied to various glycosyl chloride donors.

ORGANIC LETTERS (2022)

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Review Chemistry, Multidisciplinary

Total RNA Synthesis and its Covalent Labeling Innovation

Hongling Zhou et al.

Summary: RNA plays a critical role in physiological processes, but limited methods are available for visualizing specific RNAs. However, several efficient labeling methods for RNAs have been successfully demonstrated in recent years. Additionally, the detection of RNAs-of-interest by RNA modifying enzymes has also proved feasible. This review summarizes recent research on synthesis of RNAs-of-interest bearing unnatural nucleosides, RNA labeling research via biocompatible ligation, and other advancements in the field.

TOPICS IN CURRENT CHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Phosphorothioate-Based Site-Specific Labeling of Large RNAs for Structural and Dynamic Studies

Yanping Hu et al.

Summary: Pulsed electron-electron double resonance (PELDOR) spectroscopy, X-ray scattering interferometry (XSI), and single-molecule Forster resonance energy transfer (smFRET) are molecular rulers that provide inter- or intramolecular pair-wise distance distributions in the nanometer range, thus being ideally suitable for structural and dynamic studies of biomolecules including RNAs. However, the current labeling strategies may cause structural perturbation and decrease the accuracy of measurements.

ACS CHEMICAL BIOLOGY (2022)

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Article Chemistry, Multidisciplinary

Stronger together for in-cell translation: natural and unnatural base modified mRNA

Lisa Bornewasser et al.

Summary: In this study, we report the in-cell application of highly modified mRNA using in vitro transcription and visualization of their distribution in cells through click chemistry. By incorporating natural base modifications and site-specifically introduced artificial nucleotides, we were able to improve the translation efficiency of mRNA while maintaining its functionality. This approach provides a new way to enhance mRNA therapeutics.

CHEMICAL SCIENCE (2022)

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Review Chemistry, Multidisciplinary

Emerging Organometallic Methods for the Synthesis of C-Branched (Hetero)aryl, Alkenyl, and Alkyl Glycosides: C-H Functionalization and Dual Photoredox Approaches

Juba Ghouilem et al.

Summary: Transition-metal-catalyzed C-H functionalization and photoredox nickel dual catalysis are innovative approaches for the synthesis of C-branched glycosides, providing efficient and mild methods for accessing a variety of complex C-branched glycosides of great interest.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

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Article Chemistry, Multidisciplinary

Diastereoselective Synthesis of Aryl C-Glycosides from Glycosyl Esters via C-O Bond Homolysis

Yongliang Wei et al.

Summary: C-aryl glycosyl compounds offer improved in vivo stability compared to O- and N-glycoside analogues, making them attractive candidates for drug development and chemical biology studies. A new cross-coupling method has been developed for the preparation of C-aryl and heteroaryl glycosides, including nucleosides and 2-deoxysugars, from easily accessible glycosyl esters and bromoarenes, demonstrating the potential of this method in medicinal chemistry.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines

Kellie D. Nance et al.

Summary: The COVID-19 mRNA vaccines have utilized the modified nucleobase N1-methylpseudouridine (m1 Psi) to enhance effectiveness, contributing to one of the most efficient vaccine development campaigns. By explaining the development and function of m1 Psi in synthetic mRNAs, this article aims to promote understanding and highlight potential opportunities for chemical innovation in the future.

ACS CENTRAL SCIENCE (2021)

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Review Biochemistry & Molecular Biology

Strategies for Covalent Labeling of Long RNAs

Hannah Depmeier et al.

Summary: This review discusses recent approaches for covalent internal labeling of long RNAs, including enzymatic ligation of short synthetic oligonucleotides, synthetic biology approaches preparing site-specifically modified RNAs, and the use of DNAzymes, ribozymes, and RNA methyltransferase for RNA labeling. The potentials, limits, and future directions of these methods on long RNAs are explored.

CHEMBIOCHEM (2021)

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Article Chemistry, Multidisciplinary

Nonenzymatic Stereoselective S-Glycosylation of Polypeptides and Proteins

Li-Qiang Wan et al.

Summary: This study presents a nonenzymatic glycosylation reaction that forms axial S-glycosidic bonds under biorelevant conditions. By using allyl glycosyl sulfones as precursors to glycosyl radicals and taking advantage of the exceptional functional group tolerance of radical processes, various unprotected glycosyl units can be selectively introduced to cysteine residues of peptides. The method was successfully applied for direct glycosylation of complex polypeptides and proteins after developing a second-generation protocol, with computational studies performed to elucidate the reaction mechanism.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Biochemistry & Molecular Biology

Transcriptional processing of an unnatural base pair by eukaryotic RNA polymerase II

Juntaek Oh et al.

Summary: The study found that eukaryotic RNA polymerase II is capable of selectively recognizing unnatural base pairs with high fidelity, especially when the template strand contains dTPT3. Additionally, a novel 3'-RNA binding site termed the swing state was identified after rNaM addition. These results may pave the way for future studies in the design of transcription and translation strategies in higher organisms with expanded genetic codes.

NATURE CHEMICAL BIOLOGY (2021)

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Article Chemistry, Organic

Light-Mediated Cross-Coupling of Anomeric Trifluoroborates

Eric M. Miller et al.

Summary: This study presents stereoselective C-arylation and etherification reactions of anomeric trifluoroborates derived from BMIDA esters, exhibiting high anomeric selectivities for 2-deoxysugars and showing a broad substrate scope, including disaccharides and trifluoroborates with free hydroxyl groups. This new class of carbohydrate reagents adds to the palette of anomeric nucleophile reagents suitable for efficient installation of C-C bonds.

ORGANIC LETTERS (2021)

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Article Chemistry, Physical

Photoredox-Catalyzed Stereoselective Synthesis of C-Nucleoside Analogues from Glycosyl Bromides and Heteroarenes

Liwen Xia et al.

Summary: The synthesis of C-nucleoside analogues through photoredox-catalyzed radical coupling allows for good functional group tolerance and site- and stereoselectivities, making it applicable for coupling various monosaccharides, disaccharides, and polysaccharides with nonfunctionalized heteroarenes. Biologically important molecules can be glycosylated directly through a C-C bond using this method, and the site- and stereoselectivities can be rationalized and predicted by density functional theory (DFT) calculations.

ACS CATALYSIS (2021)

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Review Biochemistry & Molecular Biology

mRNA vaccines for COVID-19: what, why and how

Jung Woo Park et al.

Summary: The COVID-19 pandemic caused by SARS-CoV-2 has led to significant impact on human lives, with mRNA vaccines emerging as promising candidates due to their rapid development time, cost-effectiveness, versatility in design, and proven ability to induce immune response.

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES (2021)

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Review Chemistry, Multidisciplinary

The Structural Basis for Processing of Unnatural Base Pairs by DNA Polymerases

Andreas Marx et al.

CHEMISTRY-A EUROPEAN JOURNAL (2020)

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Article Chemistry, Multidisciplinary

EPR Distance Measurements on Long Non-coding RNAs Empowered by Genetic Alphabet Expansion Transcription

Christof Domnick et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

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Review Multidisciplinary Sciences

A technical review and guide to RNA fluorescence in situ hybridization

Alexander P. Young et al.

PEERJ (2020)

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Article Chemistry, Applied

Development of a Large-Scale Cyanation Process Using Continuous Flow Chemistry En Route to the Synthesis of Remdesivir

Tiago Vieira et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2020)

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Review Biochemistry & Molecular Biology

Building better polymerases: Engineering the replication of expanded genetic alphabets

Zahra Ouaray et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2020)

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Review Chemistry, Multidisciplinary

Genetic alphabet expansion technology by creating unnatural base pairs

Michiko Kimoto et al.

CHEMICAL SOCIETY REVIEWS (2020)

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Article Biochemistry & Molecular Biology

Towards Reverse Transcription with an Expanded Genetic Alphabet

Frank Eggert et al.

CHEMBIOCHEM (2019)

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Article Multidisciplinary Sciences

Hachimoji DNA and RNA: A genetic system with eight building blocks

Shuichi Hoshika et al.

SCIENCE (2019)

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Article Chemistry, Multidisciplinary

Diversity-Oriented Desulfonylative Functionalization of Alkyl Allyl Sulfones

Yong Xia et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

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Article Chemistry, Multidisciplinary

Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism

Aaron W. Feldman et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

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Article Chemistry, Multidisciplinary

Nickel-catalyzed reductive coupling of glucosyl halides with aryl/vinyl halides enabling β-selective preparation of C-aryl/vinyl glucosides

Jiandong Liu et al.

SCIENCE CHINA-CHEMISTRY (2019)

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Article Chemistry, Multidisciplinary

Development of N-Phosphinomethyl-Substituted NHC-Nickel(0) Complexes as Robust Catalysts for Acrylate Salt Synthesis from Ethylene and CO2

Kohei Takahashi et al.

CHEMISTRY-A EUROPEAN JOURNAL (2019)

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Article Biochemistry & Molecular Biology

Site-Directed Spin Labeling of RNA with a Gem-Diethylisoindoline Spin Label: PELDOR, Relaxation, and Reduction Stability

Christine Wuebben et al.

MOLECULES (2019)

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Article Chemistry, Multidisciplinary

Photogenerated acyl/alkoxycarbonyl/carbamoyl radicals for sustainable synthesis

Carlotta Raviola et al.

GREEN CHEMISTRY (2019)

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Article Chemistry, Organic

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Christof Domnick et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2019)

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Article Chemistry, Multidisciplinary

Highly stereoselective synthesis of aryl/heteroaryl-C-nucleosides via the merger of photoredox and nickel catalysis

Yingying et al.

CHEMICAL COMMUNICATIONS (2019)

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Review Chemistry, Organic

Recent advances in synthetic approaches for medicinal chemistry of C-nucleosides

Kartik Temburnikar et al.

BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY (2018)

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Review Genetics & Heredity

Fidelity of DNA replicationa matter of proofreading

Anna Bebenek et al.

CURRENT GENETICS (2018)

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Review Biochemical Research Methods

Genetic alphabet expansion biotechnology by creating unnatural base pairs

Kyung Hyun Lee et al.

CURRENT OPINION IN BIOTECHNOLOGY (2018)

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Article Biochemistry & Molecular Biology

Synthesis of New C- and N-β-D-Glucopyranosyl Derivatives of Imidazole, 1,2,3-Triazole and Tetrazole, and Their Evaluation as Inhibitors of Glycogen Phosphorylase

Sandor Kun et al.

MOLECULES (2018)

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Article Chemistry, Organic

Glycosyl Cross-Coupling with Diaryliodonium Salts: Access to Aryl C-Glycosides of Biomedical Relevance

Duk Yi et al.

ORGANIC LETTERS (2018)

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Article Chemistry, Organic

Stereoretentive Intramolecular Glycosyl Cross-Coupling: Development, Scope, and Kinetic Isotope Effect Study

Duk Yi et al.

ORGANIC LETTERS (2018)

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Review Biochemistry & Molecular Biology

Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology

Kiyofumi Hamashima et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2018)

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Article Chemistry, Multidisciplinary

Progress Toward a Semi-Synthetic Organism with an Unrestricted Expanded Genetic Alphabet

Vivian T. Dien et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

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Article Chemistry, Organic

Stereoselective Preparation of α-C-Vinyl/Aryl Glycosides via Nickel-Catalyzed Reductive Coupling of Glycosyl Halides with Vinyl and Aryl Halides

Jiandong Liu et al.

ORGANIC LETTERS (2018)

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Article Chemistry, Multidisciplinary

In Vivo Structure-Activity Relationships and Optimization of an Unnatural Base Pair for Replication in a Semi-Synthetic Organism

Aaron W. Feldman et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

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Article Chemistry, Multidisciplinary

Synthesis of Aryl C-Glycosides via Iron-Catalyzed Cross Coupling of Halosugars: Stereoselective Anomeric Arylation of Glycosyl Radicals

Laksmikanta Adak et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

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Article Biochemical Research Methods

Iluminated by foreign letters - Strategies for site-specific cyclopropene modification of large functional RNAs via in vitro transcription

Frank Eggert et al.

METHODS (2017)

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Article Biochemistry & Molecular Biology

Effective Synthesis of Nucleosides Utilizing O-Acetyl-Glycosyl Chlorides as Glycosyl Donors in the Absence of Catalyst: Mechanism Revision and Application to Silyl-Hilbert-Johnson Reaction

Chengyuan Liang et al.

MOLECULES (2017)

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Article Multidisciplinary Sciences

A semi-synthetic organism that stores and retrieves increased genetic information

Yorke Zhang et al.

NATURE (2017)

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Article Biochemistry & Molecular Biology

An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes

James S. Italia et al.

NATURE CHEMICAL BIOLOGY (2017)

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Review Chemistry, Organic

Synergy between experimental and computational studies of aromatic stacking interactions

Jung Wun Hwang et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2017)

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Article Chemistry, Organic

Glycosyl Stille Cross-Coupling with Anomeric Nucleophiles - A General Solution to a Long-Standing Problem of Stereocontrolled Synthesis of C-Glycosides

Feng Zhu et al.

SYNLETT (2017)

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Review Chemistry, Multidisciplinary

Recent Advances in the Chemical Synthesis of C-Glycosides

You Yang et al.

CHEMICAL REVIEWS (2017)

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Article Chemistry, Multidisciplinary

A New Potent Inhibitor of Glycogen Phosphorylase Reveals the Basicity of the Catalytic Site

Michael Mamais et al.

CHEMISTRY-A EUROPEAN JOURNAL (2017)

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Article Chemistry, Medicinal

C-Nucleosides To Be Revisited

Erik De Clercq

JOURNAL OF MEDICINAL CHEMISTRY (2016)

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Article Biochemistry & Molecular Biology

FRET Characterization of Complex Conformational Changes in a Large 165 Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs

Thomas Lavergne et al.

ACS CHEMICAL BIOLOGY (2016)

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Article Chemistry, Multidisciplinary

A cyclopropene-modified nucleotide for site-specific RNA labeling using genetic alphabet expansion transcription

F. Eggert et al.

CHEMICAL COMMUNICATIONS (2016)

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Article Chemistry, Multidisciplinary

Noncovalent and site-directed spin labeling of duplex RNA

Nilesh R. Kamble et al.

CHEMICAL COMMUNICATIONS (2016)

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Article Chemistry, Multidisciplinary

Highly Stereospecific Cross-Coupling Reactions of Anomeric Stannanes for the Synthesis of C-Aryl Glycosides

Feng Zhu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)

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Article Chemistry, Multidisciplinary

Alcohols as Latent Coupling Fragments for Metallaphotoredox Catalysis: sp3-sp2 Cross-Coupling of Oxalates with Aryl Halides

Xiaheng Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)

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Article Biochemical Research Methods

Aptamers: The evolution of SELEX

Yi Xi Wu et al.

METHODS (2016)

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Article Multidisciplinary Sciences

Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys

Travis K. Warren et al.

NATURE (2016)

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Article Biochemical Research Methods

High Fidelity, Efficiency and Functionalization of Ds-Px Unnatural Base Pairs in PCR Amplification for a Genetic Alphabet Expansion System

Itaru Okamoto et al.

ACS SYNTHETIC BIOLOGY (2016)

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Article Chemistry, Multidisciplinary

Dialkyl Ether Formation by Nickel-Catalyzed Cross-Coupling of Acetals and Aryl Iodides

Kevin M. Arendt et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

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Review Chemistry, Multidisciplinary

The Expanded Genetic Alphabet

Denis A. Malyshev et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

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Article Chemistry, Multidisciplinary

Site-specific enzymatic introduction of a norbornene modified unnatural base into RNA and application in post-transcriptional labeling

Christof Domnick et al.

CHEMICAL COMMUNICATIONS (2015)

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Article Chemistry, Multidisciplinary

Structural Basis for a Six Nucleotide Genetic Alphabet

Millie M. Georgiadis et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

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Article Biochemistry & Molecular Biology

Site-specific labeling of RNA by combining genetic alphabet expansion transcription and copper-free click chemistry

Tatsuhiko Someya et al.

NUCLEIC ACIDS RESEARCH (2015)

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Article Chemistry, Organic

Synthesis of novel N-glycoside derivatives via CuSCN-catalyzed reactions and their SGLT2 inhibition activities

Shao-Tao Bai et al.

TETRAHEDRON (2015)

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Article Chemistry, Multidisciplinary

Natural-like Replication of an Unnatural Base Pair for the Expansion of the Genetic Alphabet and Biotechnology Applications

Lingjun Li et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

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Article Multidisciplinary Sciences

A semi-synthetic organism with an expanded genetic alphabet

Denis A. Malyshev et al.

NATURE (2014)

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Review Pharmacology & Pharmacy

The Major Facilitative Folate Transporters Solute Carrier 19A1 and Solute Carrier 46A1: Biology and Role in Antifolate Chemotherapy of Cancer

Larry H. Matherly et al.

DRUG METABOLISM AND DISPOSITION (2014)

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Article Chemistry, Organic

Cobalt-Catalyzed Diastereoselective Synthesis of C-Furanosides. Total Synthesis of (-)-Isoaltholactone

Lionel Nicolas et al.

JOURNAL OF ORGANIC CHEMISTRY (2013)

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Article Chemistry, Multidisciplinary

Expanding the Scope of Replicable Unnatural DNA: Stepwise Optimization of a Predominantly Hydrophobic Base Pair

Thomas Lavergne et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

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Article Chemistry, Multidisciplinary

Structural Insights into DNA Replication without Hydrogen Bonds

Karin Betz et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

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Article Biotechnology & Applied Microbiology

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

Michiko Kimoto et al.

NATURE BIOTECHNOLOGY (2013)

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Article Biochemistry & Molecular Biology

KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Karin Betz et al.

NATURE CHEMICAL BIOLOGY (2012)

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Article Biochemistry & Molecular Biology

Highly specific unnatural base pair systems as a third base pair for PCR amplification

Rie Yamashige et al.

NUCLEIC ACIDS RESEARCH (2012)

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Article Chemistry, Organic

Stereoselective C-Glycosylation Reactions with Arylzinc Reagents

Sebastien Lemaire et al.

ORGANIC LETTERS (2012)

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Article Chemistry, Multidisciplinary

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

Ivan Krstic et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)

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Article Chemistry, Multidisciplinary

Site-Specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs

Young Jun Seo et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

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Article Multidisciplinary Sciences

Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging

Jia Guo et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

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Article Chemistry, Multidisciplinary

Relative Orientation of Rigid Nitroxides by PELDOR: Beyond Distance Measurements in Nucleic Acids

Olav Schiemann et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)

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Review Chemistry, Multidisciplinary

C-Nucleosides: Synthetic Strategies and Biological Applications

Jan Stambasky et al.

CHEMICAL REVIEWS (2009)

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Review Biochemistry & Molecular Biology

Polymerase Dynamics at the Eukaryotic DNA Replication Fork

Peter M. J. Burgers

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

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Article Chemistry, Physical

Noncovalent Interactions Involving Histidine: The Effect of Charge on π-π Stacking and T-Shaped Interactions with the DNA Nucleobases

Cassandra D. M. Churchill et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2009)

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Article Biochemistry & Molecular Biology

An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Michiko Kimoto et al.

NUCLEIC ACIDS RESEARCH (2009)

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Article Chemistry, Physical

An application of the van der Waals density functional: Hydrogen bonding and stacking interactions between nucleobases

Valentino R. Cooper et al.

JOURNAL OF CHEMICAL PHYSICS (2008)

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Article Chemistry, Multidisciplinary

Discovery, characterization, and optimization of an unnatural base pair for expansion of the genetic alphabet

Aaron M. Leconte et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)

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Article Chemistry, Multidisciplinary

Double-stranded DNA dissociates into single strands when dragged into a poor solvent

Shuxun Cui et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)

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Article Biochemistry & Molecular Biology

Base-specific spin-labeling of RNA for structure determination

Nelly Piton et al.

NUCLEIC ACIDS RESEARCH (2007)

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Article Chemistry, Multidisciplinary

A room temperature negishi cross-coupling approach to C-alkyl glycosides

Hegui Gong et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)

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Article Biochemical Research Methods

Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances

Olav Schiemann et al.

NATURE PROTOCOLS (2007)

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Review Biochemical Research Methods

Methods developed for SELEX

Subash Chandra Bose Gopinath

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