期刊
BIOGERONTOLOGY
卷 -, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1007/s10522-023-10044-z
关键词
Pax6; Aging; Pan-Ras; Raf; ERK1; 2
This study aims to evaluate the expression patterns of Pax6 and its interaction with Ras, Raf, and ERK1/2 in different brain regions of mice. The results indicate age-dependent changes of Pax6, Ras, Raf, and ERK1/2 in different regions of the brain. ERK1/2 shows synergistic activities with Pax6.
Pax6, a transcription factor and multifunctional protein, changes during aging. It also interacts with regulator proteins involved in cell metabolism and survival signalling pathways including Ras-GAP. Many forms of Ras, Raf and ERK1/2 are known but information on their region-specific expression patterns are unavailable from brain during aging. Therefore, it has been intended to evaluate expressions of Pax6 and forms of Ras, Raf, ERK1/2 in hippocampus, caudate nucleus, amygdale, cerebral cortex, cerebellum and olfactory lobe. Association of Pax6 with Ras, Raf and ERK1/2 was evaluated in co-culture (PC-12, C6-glia, U-87 MG) of neuroglia cell lines. Impacts of Pax6 were evaluated by siRNA mediated knockdown and expression patterns Ras-Raf-Erk1/2. Analysis of activities of Pax6 and impacts of 5 & PRIME;AMP, wild-type and mutant ERK were done by RT-PCR and luciferase reporter assay. Results indicate age-dependent changes of Pax6, Ras, Raf, ERK1/2 in different regions of brain of young and old mice. Erk1/2 shows synergistic activities to Pax6.
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