Rapid and specific detection of Chinook salmon bafinivirus (CSBV) in flatfish using loop-mediated isothermal amplification (LAMP)

It is now understood that the flatfish farming industry faces significant threats from Chinook salmon bafinivirus (CSBV) induced hemorrhagic disease. Developing a prompt and efficient diagnostic method is crucial for effective disease control and minimizing losses in aquaculture production. This research assessed the loop-mediated isothermal amplification (LAMP) technique, using a specific primer set, for rapid and precise detection of CSBV. The primer set for LAMP detection was selected based on its demonstrated high specificity and sensitivity, as confirmed through visualization and gel electrophoresis. The CSBV-LAMP reaction system operated at an effective temperature of 63-67 & DEG;C for 60 min. Importantly, the LAMP assay could identify viral genomic cDNA at an extremely low concentration of 10 pg/& mu;L within 60 min at 65 & DEG;C. Moreover, this reaction system accurately detected CSBV in naturally infected flatfish samples, yielding a 100% positive detection rate.

Automated summary

It has been found that the flatfish farming industry is threatened by Chinook salmon bafinivirus (CSBV) induced hemorrhagic disease. Therefore, the development of a rapid and efficient diagnostic method is crucial for disease control in aquaculture. This research evaluated the loop-mediated isothermal amplification (LAMP) technique for the detection of CSBV, and it showed high specificity and sensitivity, with a positive detection rate of 100% in naturally infected flatfish samples.