4.7 Article

Insight into biomolecular interaction-based non-classical crystallization of bacterial biocement

期刊

出版社

SPRINGER
DOI: 10.1007/s00253-023-12736-5

关键词

Bacteria; Calcium carbonate; Biomineral; Extracellular polymeric substances; Nucleation; Biocement

向作者/读者索取更多资源

This study aimed to explore the correlation between organic biomolecules and calcium carbonate (CaCO3) precipitation, and it found that carbohydrates and proteins on the cell surface played a vital role in the nucleation and polymorphism of CaCO3. The research also showed that calcite stabilized at 30 days in ureolysis, while a mix of vaterite and calcite occurred in ammonification. The findings contribute to a better understanding of enzymes and EPS-driven non-classical nucleation of CaCO3.
In an attempt to draw a correlation between calcium carbonate (CaCO3) precipitation and biomacromolecules such as extracellular polymeric substances and enzyme activity in biomineralizing microbe, this report aims to elucidate the ureolytic and ammonification route in Paenibacillus alkaliterrae to explore the possible role of organic biomolecule(s) present on cell surface in mediating nucleation and crystallization of biogenic CaCO3. After 168 h of biomineralization in ureolysis and ammonification, 2.2 g/l and 0.87 g/l of CaCO3 precipitates were obtained, respectively. The highest carbonic anhydrase activity (31.8 & mu;moles/min/ml) was evidenced in ammonification as opposed to ureolysis (24.8 & mu;moles/min/ml). Highest urease activity reached up to 9.26 & mu;moles/min/ml in ureolytic pathway. Extracellular polymeric substances such as polysaccharides and proteins were found to have a vital role not only in the nucleation and crystal growth but also in addition direct polymorphic fate of CaCO3 nanoparticles. EPS production was higher during ammonification (3.1 mg/ml) than in ureolysis (0.72 mg/ml). CaCO3 nanoparticle-associated proteins were found to be 0.82 mg/ml in ureolysis and 0.56 mg/ml in ammonification. After 30 days of biomineralization, all the polymorphic forms stabilized to calcite in ureolysis but in ammonification vaterite predominated. In our study, we showed that organic template-mediated prokaryotic biomineralization follows the non-classical nucleation and varying proportions of these organic components causes selective polymorphism of CaCO3 nanoparticles. Overall, the findings are expected to further the fundamental understanding of enzymes, EPS-driven non-classical nucleation of CaCO3, and we foresee the design of fit-for-purpose futuristic biominerals arising from such renewed understanding of biomineralization.Key points & BULL; Organic-inorganic interface of cell surface promote crystallization of biominerals & BULL; Carbohydrate and proteins in the interface results selective polymorphism of CaCO3 & BULL; Calcite stabilized at 30 days in ureolysis, vaterite-calcite mix in ammonificationKey points & BULL; Organic-inorganic interface of cell surface promote crystallization of biominerals & BULL; Carbohydrate and proteins in the interface results selective polymorphism of CaCO3 & BULL; Calcite stabilized at 30 days in ureolysis, vaterite-calcite mix in ammonificationKey points & BULL; Organic-inorganic interface of cell surface promote crystallization of biominerals & BULL; Carbohydrate and proteins in the interface results selective polymorphism of CaCO3 & BULL; Calcite stabilized at 30 days in ureolysis, vaterite-calcite mix in ammonification

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据