Biosensing systems for the detection and quantification of methane gas

Climate change due to the continuous increase in the release of green-house gasses associated with anthropogenic activity has made a significant impact on the sustainability of life on our planet. Methane (CH4) is a green-house gas whose concentrations in the atmosphere are on the rise. CH4 measurement is important for both the environment and the safety at the industrial and household level. Methanotrophs are distinguished for their unique characteristic of using CH4 as the sole source of carbon and energy, due to the presence of the methane monooxygenases that oxidize CH4 under ambient temperature conditions. This has attracted interest in the use of methanotrophs in biotechnological applications as well as in the development of biosensing systems for CH4 quantification and monitoring. Biosensing systems using methanotrophs rely on the use of whole microbial cells that oxidize CH4 in presence of O-2, so that the CH4 concentration is determined in an indirect manner by measuring the decrease of O-2 level in the system. Although several biological properties of methanotrophic microorganisms still need to be characterized, different studies have demonstrated the feasibility of the use of methanotrophs in CH4 measurement. This review summarizes the contributions in methane biosensing systems and presents a prospective of the valid use of methanotrophs in this field.

Automated summary

Climate change due to increased greenhouse gas emissions has had a significant impact on the sustainability of life on Earth. Methane, a greenhouse gas, is increasing in concentration and its measurement is important for the environment and safety. The use of methane-oxidizing bacteria in biosensing systems shows promise for methane quantification and monitoring.