Environmental modulation of exopolysaccharide production in the cyanobacterium Synechocystis 6803

Microorganisms produce extracellular polymeric substances (EPS, also known as exopolysaccharides) of diverse composition and structure. The biochemical and biophysical properties of these biopolymers enable a wide range of industrial applications. EPS from cyanobacteria are particularly versatile as they incorporate a larger number and variety of building blocks and adopt more complex structures than EPS from other organisms. However, the genetic makeup and regulation of EPS biosynthetic pathways in cyanobacteria are poorly understood. Here, we measured the effect of changing culture media on titre and composition of EPS released by Synechocystis sp. PCC 6803, and we integrated this information with transcriptomic data. Across all conditions, daily EPS productivity of individual cells was highest in the early growth phase, but the total amount of EPS obtained from the cultures was highest in the later growth phases due to accumulation. Lowering the magnesium concentration in the media enhanced per-cell productivity but the produced EPS had a lower total sugar content. Levels of individual monosaccharides correlated with specific culture media components, e.g. xylose with sulfur, glucose and N-acetyl-galactosamine with NaCl. Comparison with RNA sequencing data suggests a Wzy-dependent biosynthetic pathway and a protective role for xylose-rich EPS. This multi-level analysis offers a handle to link individual genes to the dynamic modulation of a complex biopolymer.Graphical Abstract

Automated summary

Microorganisms produce diverse extracellular polymeric substances (EPS), which have a wide range of industrial applications. Cyanobacterial EPS are particularly versatile with complex structures. However, understanding the genetic makeup and regulation of EPS biosynthetic pathways in cyanobacteria is limited. This study measured the effect of changing culture media on EPS production and composition in Synechocystis sp. PCC 6803, and integrated this information with transcriptomic data. The analysis revealed a Wzy-dependent biosynthetic pathway and a protective role for xylose-rich EPS.