期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 -, 期 -, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202315185
关键词
Aptamer; Biosensing; Litmus Test; Rolling Circle Amplification; Urease
Here we report on an ultra-sensitive colorimetric sensing platform that combines the amplification power of rolling circle amplification (RCA) with the efficiency of a urease-mediated litmus test. By utilizing aptamers for target recognition, this platform can be adapted for a wide range of sensing applications.
Here we report on an ultra-sensitive colorimetric sensing platform that takes advantage of both the strong amplification power of rolling circle amplification (RCA) and the high efficiency of a simple urease-mediated litmus test. The presence of a target triggers the RCA reaction, and urease-labelled DNA can hybridize to the biotinylated RCA products and be immobilized onto streptavidin-coated magnetic beads. The urease-laden beads are then used to hydrolyze urea, leading to an increase in pH that can be detected by a simple litmus test. We show this sensing platform can be easily integrated with aptamers for sensing diverse targets via the detection of human thrombin and platelet-derived growth factor (PDGF) utilizing structure-switching aptamers as well as SARS-CoV-2 in human saliva using a spike-binding trimeric DNA aptamer. Furthermore, we demonstrate that this colorimetric sensing platform can be integrated into a simple paper-based device for sensing applications. Sensitive and simple-to-use colorimetric sensors are highly desirable for point-of-care diagnostic applications. Here we report on an ultra-sensitive colorimetric sensing platform that takes advantage of both the strong amplification power of rolling circle amplification and the high efficiency of a urease-mediated litmus test. By using aptamers for target recognition, this platform can be adapted for a wide range of sensing applications.+image
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