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Article
Medicine, General & Internal
Christopher H. van Dyck et al.
Summary: Lecanemab, a humanized monoclonal antibody, reduced amyloid markers and showed improvement in cognition and function in early Alzheimer's disease patients. However, it also resulted in adverse events. Longer trials are needed to determine its efficacy and safety in early Alzheimer's disease.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Chemistry, Analytical
Luofeng Su et al.
Summary: This work presents a novel amplification strategy, tsBMRP, for the electrochemical aptamer-based detection of thrombin at femtomolar levels. The tsBMRP-based strategy is biologically friendly, low-cost, and simple in operation. The electrochemical aptasensor based on the tsBMRP strategy shows high sensitivity and selectivity, making it promising for biomedical and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: By taking advantage of target-assisted electrochemically mediated atom transfer radical polymerization (teATRP) for signal amplification, a simple and cost-effective electrochemical aptasensor is developed for sensitive and selective detection of low-abundance endotoxin. The aptamer receptor selectively captures endotoxin, and signal amplification is achieved through the dual amplification strategy of ATRP-initiated polymer chain grafting. This aptasensor shows high sensitivity with a detection limit of 1.2 fg/mL and has great potential for detecting low-abundance endotoxin and other glycan chain containing bio-targets.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Baubek Spanov et al.
Summary: The study found that Pertuzumab undergoes changes in charge heterogeneity, primarily due to deamidation in the Fc domain and N-terminal pyroglutamate formation in the heavy chain. Peptide mapping analysis also showed that clinical samples had 2-3% deamidation in the heavy chain CDR2, 20-25% deamidation in the Fc domain, and 10-15% N-terminal pyroglutamate formation in the heavy chain.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Linghui Qian et al.
Summary: The high selectivity and affinity of antibodies have made them valuable tools in disease therapy, diagnosis, and basic research. Various chemical and genetic approaches have been developed to improve their effectiveness and expand their applications in areas such as targeted protein degradation, real-time live-cell imaging, and catalytic labeling or decaging. Advancements in chemistry and biotechnology have led to well-designed antibodies and their derivatives that improve our understanding of biological processes and offer potential treatments for various diseases.
Review
Urology & Nephrology
Naoka Murakami et al.
Summary: This article evaluates different types of therapies for treating COVID-19, with a particular focus on their relevance to patients with kidney failure and kidney transplant recipients. The research finds that anti-inflammatory and antiviral agents have a stronger evidence base and greater benefits compared to other therapeutic approaches.
NATURE REVIEWS NEPHROLOGY
(2023)
Review
Chemistry, Analytical
Jing Pan et al.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Felix Kuhne et al.
Summary: Antibody combination therapies have emerged as viable treatment options for severe diseases like cancer. However, the co-formulation production approach poses challenges for the analytical control of drug product quality due to complex variant profiles. This study developed a multi-dimensional liquid chromatography with tandem mass spectrometry method to characterize antibody homo- and hetero-aggregates. The results showed that the co-formulation of trastuzumab and pertuzumab, two monoclonal antibodies with similar properties, exhibited high stability with minimal aggregate formation under storage and stress conditions. The formation of homo- and hetero-aggregates was not significantly affected by formulation and storage conditions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sara Bracaglia et al.
Summary: Research scientists have developed programmable antigen-conjugated gene circuits that can detect target antibodies and trigger the production of detectable RNA sequences using a disposable electrochemical sensor. This cell-free electrochemical biosensor enables sensitive, specific, selective, and multiplexed detection of different antibodies in blood serum.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Oncology
Sacha Zinn et al.
Summary: In this review article, Christ and colleagues discuss recent developments in antibody-based cancer therapies. Monoclonal antibodies, characterized by their specificity, long serum half-life, high affinity, and immune effector functions, are an ever-growing class of targeted cancer therapeutics. The authors outline key advances in the field, focusing on engineered antibody therapeutic candidates, and provide updates on their molecular structure, mechanisms of action, clinical development, and practice.
Article
Chemistry, Analytical
Katie M. Kilgour et al.
Summary: The global pandemic caused by SARS-CoV-2 has had a significant impact on healthcare systems worldwide. The development of rapid and accurate tests to detect anti-SARS-CoV-2 antibodies is crucial for tracking the spread of variants and supporting the manufacturing and administration of therapeutic antibodies. This study evaluates the performance of the DARQ assay for quantifying anti-SARS-CoV-2 antibodies in different sample types and demonstrates its reproducibility, rapidity, and independence from sample complexity. The DARQ assay is a valuable tool for monitoring anti-SARS-CoV-2 antibodies.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Emma Campbell et al.
Summary: The study demonstrates a method using an enzyme switch sensor platform for rapid and sensitive quantification of two monoclonal antibody therapies. This method successfully monitored the two therapies within the therapeutic range in human serum.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Hui Yin Tan et al.
Summary: This research presents a method for rapid quantitation of therapeutic monoclonal antibodies (mAbs) using affinity glass-fiber membranes in a 96-well-plate format. The membrane adsorbs a film containing poly(acrylic acid), allowing the capture of mAbs through covalent-linking. The captured mAbs are then detected using fluorescence intensity, providing a convenient and quick way to quantify mAbs with low variability.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Rui Han et al.
Summary: In this study, an antifouling electrochemical biosensor was developed for direct detection of circulating tumor cells (CTCs) in blood, without the need for purification or separation. The biosensor, integrated with a multifunctional peptide and conducting polymer PEDOT, exhibited enhanced sensitivity and retained linear response even in the presence of biofouling in real blood samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lingqi Kong et al.
Summary: A novel ratiometric electrochemical biosensor with methylene blue (MB) as the only signal tag was developed for highly reliable and ultrasensitive detection of microRNA-21 (miRNA-21). The strategy utilized an intelligent target-induced dual signal amplification (T-DSA) to controllably load and release MB, avoiding false positive responses. With the aid of innovative T-DSA recycle and rolling circle amplification (RCA)-produced functional DNA nanospheres, the detection sensitivity of this biosensor was significantly improved.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haoran Tang et al.
Summary: In this study, functionalized-nanopore-based single-entity electrochemistry analysis tools were used to monitor the single-molecule glycoprotein-boronate affinity interaction. The results showed that the equilibrium association constant of this interaction in a confined nanopore was larger than that in an open system, possibly due to enhanced multivalent synergistic binding. Additionally, the method also demonstrated potential application value for the label-free detection of glycoproteins in real samples, paving the way for single-cell analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Aron Gyorgypal et al.
Summary: The biopharmaceutical industry is adopting continuous biomanufacturing practices and advanced process analytical technology to improve flexibility and efficiency in production. N-GLYcanyzer, an online sequential-injection-based PAT system, has been developed to monitor mAb glycosylation during biomanufacturing, allowing for real-time analysis and optimization.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Taro Saito et al.
Summary: A novel anti-idiotype aptamer A14#1 with extraordinary specificity against the anti-vascular endothelial growth factor therapeutic mAb, bevacizumab, was developed. The affinity of A14#1 to bevacizumab markedly increased at pH 4.7, enabling it to be potentially useful for therapeutic drug measurement as a novel ligand of bevacizumab.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: A boronate-affinity cross-linking method is demonstrated for the highly sensitive and selective detection of glycoconjugates. The method utilizes a methylene blue-tagged nucleic acid aptamer to selectively capture target glycoconjugates, followed by labeling a large number of ferrocene tags through boronate-affinity cross-linking. The dual-signal output from the methylene blue and ferrocene tags enables ratiometric detection. This method is simple, cost-effective, and possesses high robustness and reproducibility.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: Researchers have developed a universal boronate affinity-based electrochemical aptasensor for the detection of glycoproteins. The sensor is highly sensitive and selective, with tolerance for false-positive results. It offers the advantages of simple operation, short assay time, and low detection cost, and can be used for the detection of glycoproteins in serum samples and point-of-care detection using disposable flexible electrodes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: Polymers have great potential as carriers for signal tags in amplified detection. This study presents a polysaccharide-amplified method for electrochemical detection of a specific DNA target. The method involves attaching carboxyl group containing polysaccharides to a peptide nucleic acid capture probe, followed by the decoration of polysaccharide chains with electroactive ferrocene signal tags. The method shows high sensitivity, selectivity, and cost-effectiveness.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: A simple and cost-friendly electrochemical aptasensor was developed for sensitive and selective detection of glycoprotein tumor biomarkers. The aptasensor showed high sensitivity and selectivity, and was successfully applied to detect glycoprotein tumor biomarkers in human serum samples. This eATRP-based aptasensor has great potential in the glycoprotein-based liquid biopsy of malignant tumors, even in the early stage.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Mengzhen Lv et al.
Summary: A novel electrochemical biosensor based on MIL-101-NH2 (Cr) was developed to detect p53 gene by combining target-responsive releasing and self-catalysis strategies. The biosensor exhibited a wide linear range and low detection limit, offering a promising approach for constructing ultra-sensitive and simple biosensors.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Qiong Hu et al.
Summary: As lipopolysaccharide (LPS) is closely associated with sepsis and other life-threatening conditions, the development of a point-of-care (POC) detection method for LPS is highly important. This study introduces an electrochemical aptasensor that utilizes boronate affinity (BA) as an amplification strategy for the POC detection of low-abundance LPS. The BA-mediated signal amplification offers the advantages of simplicity, rapidness, and cost-effectiveness over conventional strategies based on nanomaterials and enzymes. The developed aptasensor successfully detects LPS within 45 minutes, with a detection limit of 0.34 pg/mL, and demonstrates its potential applicability in POC testing.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Luis Lopez et al.
Summary: The study demonstrated the measurement of Neuropeptide Y using aptamer-modified microelectrodes by electrochemical impedance spectroscopy. The method showed high sensitivity, specificity, and a wide linear range for NPY detection without the need for a redox reaction. The immobilization and biofouling of the microelectrodes were studied and compared, with changes in impedance factors directly correlated with NPY concentrations.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Shengfeng Huang et al.
Summary: This study developed a high-performance anti-fouling coating based on poly adenine (polyA(n)) and a highly specific CD20 epitope mimetic peptide (CN14) to construct a facile biosensor for rapid determination of rituximab in lymphoma patients' plasma. The optimized polyA(n) coating showed excellent stability and anti-fouling performance, demonstrating better results for handling complex clinical plasma samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Joshua D. Berwanger et al.
Summary: Effective monoclonal antibody (mAb) therapies require a specific serum concentration of mAbs to be effective and avoid side effects. This study used microporous nylon membranes functionalized with antibody-binding peptides to capture mAbs from diluted patient sera, allowing for quantitation using native fluorescence. Future work aims to develop rapid point-of-care devices and decrease detection limits for more successful treatments.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Matthew J. Russo et al.
Summary: Electrochemical biosensors offer a promising approach for rapid on-the-spot sample analysis, but the main issue lies in developing platforms with high sensitivity and selectivity for analyzing low analyte concentrations in complex biological samples, due to nonspecific adsorption at the electrode interface.
Article
Chemistry, Multidisciplinary
Sara Bracaglia et al.
Summary: The electrochemical DNA circuit designed for detecting specific antibodies is sensitive, specific, and selective, suitable for use in complex media. This versatile circuit can detect multiple different antibodies and conduct multiplexed detection without crosstalk.
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ANALYTICAL CHEMISTRY
(2016)
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Daojin Li et al.
CHEMICAL SOCIETY REVIEWS
(2015)
Review
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Emanual Maverakis et al.
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R Singh et al.
