相关参考文献
注意：仅列出部分参考文献，下载原文获取全部文献信息。
Article
Microbiology
Sara Moron-Lopez et al.
Summary: We developed a one-step reverse transcription (RT)-droplet digital PCR (ddPCR) method to detect SARS-CoV-2 RNA and compared it to the gold-standard RT-quantitative PCR (RT-qPCR) method. RT-ddPCR was more sensitive than RT-qPCR in detecting low viral loads, while both techniques performed equally in mid- and high-viral-load ranges. Thus, RT-ddPCR could be an alternative for detecting low viral loads and assessing viral persistence in post-COVID patients.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Lucas J. Osborn et al.
Summary: This study evaluated the clinical utility of SARS-CoV-2 sgRNA RT-PCR testing in a pediatric population. The results showed significant associations between sgRNA RT-PCR results and clinical parameters related to COVID-19, guiding patient management and infection prevention and control practices.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Medical Laboratory Technology
Mei Yang et al.
Summary: This study evaluated the performance of five commercial RT-qPCR diagnostic kits for SARS-CoV-2 used in China. The results showed that all kits passed the performance verification, except for one. Da An and Liferiver demonstrated excellent clinical diagnostic specificity and sensitivity. This study can guide the selection or optimization of RT-qPCR diagnostic test kits for SARS-CoV-2.
CLINICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Laura Vierbaum et al.
Summary: To track the circulation of SARS-CoV-2, reliable diagnostic methods are required. However, interlaboratory studies have shown high variability in the reported Ct values. In order to estimate the viral load, standards were introduced, and reference materials containing heat-inactivated SARS-CoV-2 were established. These reference materials proved to be a suitable tool for determining the specific laboratory Ct for a given viral load and could also be used as run controls to ensure the reproducibility of quantitative measurements.
Article
Ophthalmology
Maria Casagrande et al.
Summary: In deceased patients with COVID-19, SARS-CoV-2 RNA was detected in retinal biopsies and optic nerve biopsies, but virus isolation failed and immunohistochemistry of the SARS-CoV-2 spike protein was negative. Detection of subgenomic RNA in the tissues suggests a possible infection of neuronal tissue, but an active infection seems unlikely.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Biochemical Research Methods
Sushama Telwatte et al.
Summary: This study describes a novel panel of reverse transcription droplet digital PCR assays that can specifically quantify multiple different subgenomic SARS-CoV-2 transcripts and distinguish them from non-discontinuous transcripts. These assays can be applied to samples from infected patients to better understand the regulation of SARS-CoV-2 transcription and the contribution of different sgRNAs to viral pathogenesis and clinical disease severity.
Review
Microbiology
Raphael Nyaruaba et al.
Summary: The ongoing COVID-19 pandemic caused by SARS-CoV-2 has created a global public health crisis. While RT-qPCR is the current gold standard for diagnosis, dPCR has shown to be more effective in detecting low viral loads. Understanding the applications of dPCR will help shape future diagnosis and monitoring of infectious disease outbreaks.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Biochemical Research Methods
Shawn Yi Han Tan et al.
Summary: The study evaluated the optimized dPCR assay on the Clarity PlusTM dPCR system for absolute quantification of SARS-CoV-2. The results demonstrated good precision, accuracy, and linearity, with slightly higher sensitivity compared to qPCR.
Article
Biochemical Research Methods
Chunyan Niu et al.
Summary: This study established a new method to quantify SARS-CoV-2 RNA without the need for reverse transcription. Compared to traditional methods, this approach offers higher reliability and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Public, Environmental & Occupational Health
Beatriz Boger et al.
Summary: This study systematically reviewed the accuracy parameters of different diagnostic methods for detecting SARS-CoV-2, finding that computed tomography has high sensitivity but low specificity, while the combination of IgM and IgG antibodies showed promising results. The research also indicated that RT-PCR remains the gold standard for diagnosing COVID-19 in sputum samples, but combining different diagnostic methods can improve accuracy.
AMERICAN JOURNAL OF INFECTION CONTROL
(2021)
Article
Multidisciplinary Sciences
Yaara Finkel et al.
Summary: A high-resolution map of coding regions in the SARS-CoV-2 genome enables the identification of 23 unannotated open reading frames and quantification of the expression of canonical viral open reading frames.
Article
Biochemical Research Methods
Chunyan Niu et al.
Summary: The COVID-19 pandemic has caused serious harm to global health. RT-dPCR has shown good consistency and comparability in quantifying SARS-CoV-2 RNA, demonstrating its potential for virus detection and testing capabilities in participating laboratories.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Pathology
Raphael Nyaruaba et al.
Summary: This study demonstrates the development of different ddPCR SAR-CoV-2 assays, including multiplex assays. Results show that the quadruplex assay has similar detection limits and accuracy to lower multiplex assays, and the triplex probe mix assay has better sensitivity than the RT-qPCR assay. Additionally, the ddPCR multiplex assay indicates remdesivir's ability to inhibit SARS-CoV-2 growth in vitro.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Virology
Gabriel Dagotto et al.
Summary: Respiratory virus challenge studies aim to differentiate actively replicating virus from input challenge virus in the same anatomical locations. This study explores the potential utility of using subgenomic RNA to monitor actively replicating virus of SARS-CoV-2.
JOURNAL OF VIROLOGY
(2021)
Article
Chemistry, Analytical
Lianhua Dong et al.
Summary: The COVID-19 outbreak caused by a novel Coronavirus has spread globally. Current gold standard RT-qPCR for SARS-CoV-2 diagnosis shows varying sensitivity, highlighting the need for more accurate methods. A study on RT-dPCR demonstrated significant improvement in detection sensitivity, particularly for asymptomatic patients, making it a highly accurate method for screening suspected cases.
Article
Pathology
Jing Xu et al.
Summary: The clinical validation of the Bio-Rad SARS-CoV-2 ddPCR Kit showed high sensitivity and accuracy in detecting SARS-CoV-2 viral RNA. This method could provide definitive evaluation for borderline positive cases or suspected false-negative cases, indicating its potential in clinical applications.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Immunology
Derek E. Dimcheff et al.
Summary: The study investigated the detection of total and subgenomic RNA of SARS-CoV-2 in nasopharyngeal swab samples and their relationship to symptom duration. Results showed that subgenomic RNA levels declined linearly with total RNA levels, with a constant relationship over the duration of symptoms. This suggests that subgenomic RNA may not provide additional information on infectivity beyond what can be determined by total RNA copies.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Stephen Bustin et al.
Summary: RT-qPCR is a widely-used tool for diagnosing RNA pathogens, particularly in the context of COVID-19 testing. Despite some limitations, it has shown robustness in detecting SARS-CoV-2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Jianing Yang et al.
Summary: This study presents a portable microfluidic-based integrated detection-analysis system for SARS-CoV-2 nucleic acids detection directly from saliva samples. It offers a rapid, sensitive, and automated method for detecting virus infection, with the ability to detect 1000 copies/mL of SARS-CoV-2 RNA or virus.
INFECTIOUS DISEASE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yixuan J. Hou et al.
Article
Biochemistry & Molecular Biology
Dongwan Kim et al.
Article
Immunology
Xinjin Liu et al.
EMERGING MICROBES & INFECTIONS
(2020)
Article
Multidisciplinary Sciences
Roman Woelfel et al.
Article
Genetics & Heredity
Andrew D. Davidson et al.
Review
Health Care Sciences & Services
Bertalan Nemeth et al.
EXPERT REVIEW OF PHARMACOECONOMICS & OUTCOMES RESEARCH
(2018)
Article
Virology
Isabel Sola et al.
ANNUAL REVIEW OF VIROLOGY, VOL 2
(2015)
Article
Medical Laboratory Technology
Jim F. Huggett et al.
CLINICAL CHEMISTRY
(2013)
Article
Biochemical Research Methods
Sophie J. Smither et al.
JOURNAL OF VIROLOGICAL METHODS
(2013)
Article
Biochemical Research Methods
Suzanne Weaver et al.
