A dual channel rhodamine appended smart probe for selective recognition of Cu2+and Hg2+via turn on optical readout

A new rhodamine-6G hydrazone RHMA has been synthesized using rhodamine-6G hydrazide and 5-Allyl-3methoxysalicylaldehyde. RHMA has been fully characterized with different spectroscopic methods and single crystal XRD. RHMA can selectively recognize Cu2+ and Hg2+ in aqueous media amongst other common competitive metal ions. A significant change in absorbance was observed with Cu2+ and Hg2+ ions with emergence of a new peak at lambda max 524 nm and 531 nm respectively. Hg2+ ions lead to turn-on fluorescence enhancement at lambda max 555 nm. This event of absorbance and fluorescence marks the opening of spirolactum ring causing visual color change from colorless to magenta and light pink.RHMA-Cu2 thorn and RHMA- Hg2 thorn complexes are found to be reversible in presence of EDTA2-ions. RHMA has real application in form of test strip. Additionally, the probe exhibits turn-on readout-based sequential logic gate-based monitoring of Cu2+ and Hg2+ at ppm levels, which may be able to address real-world challenges through simple synthesis, quick recovery, response in water, by-eye detection, reversible response, great selectivity, and a variety of output for accurate investigation.

Automated summary

A new rhodamine-6G hydrazone (RHMA) was synthesized using rhodamine-6G hydrazide and 5-Allyl-3methoxysalicylaldehyde. RHMA was fully characterized and showed selective recognition of Cu2+ and Hg2+ among other metals. The absorbance and fluorescence of RHMA changed significantly with the presence of Cu2+ and Hg2+, resulting in a visual color change. RHMA also showed reversibility in the presence of EDTA2- ions and can be applied as a test strip for detection.