In-situ detection scheme for EGFR gene with temperature and pH compensation using a triple-channel optical fiber biosensor

An advanced multi-parameter optical fiber sensing technology for EGFR gene detection based on DNA hybrid-ization technology is demonstrated in this paper. For traditional DNA hybridization detection methods, tem-perature and pH compensation can not be realized or need multiple sensor probes. However, the multi-parameter detection technology we proposed can simultaneously detect complementary DNA, temperature and pH based on a single optical fiber probe. In this scheme, three optical signals including dual surface plasmon resonance signal (SPR) and Mach-Zehnder interference signal (MZI) are excited by binding the probe DNA sequence and pH -sensitive material with the optical fiber sensor. The paper proposes the first research to achieve simultaneous excitation of dual SPR signal and Mach-Zehnder interference signal in a single fiber and used for three-parameter detection. Three optical signals have different sensitivities to the three variables. From a mathematical point of view, the unique solutions of exon-20 concentration, temperature and pH can be obtained by analyzing the three optical signals. The experimental results show that the exon-20 sensitivity of the sensor can reach 0.07 nm nM-1, and the limit of detection is 3.27 nM. The designed sensor gives a fast response, high sensitivity, and low detection limit, which is important for the field of DNA hybridization research and for solving the problems of biosensor susceptibility to temperature and pH.

Automated summary

This paper demonstrates an advanced multi-parameter optical fiber sensing technology for EGFR gene detection based on DNA hybridization technology. The proposed multi-parameter detection technology can simultaneously detect complementary DNA, temperature, and pH based on a single optical fiber probe, overcoming the limitations of traditional DNA hybridization detection methods. The designed sensor has a sensitivity of 0.07 nm nM-1 for exon-20 concentration and a detection limit of 3.27 nM, providing a fast response, high sensitivity, and low detection limit that is important for DNA hybridization research and addressing the issues of biosensor susceptibility to temperature and pH.