Aggregation-induced electrochemiluminescence enhancement of Ag-MOG for amyloid β 42 sensing

This study aimed to introduce an immunosensor for measuring amyloid beta 42 (A beta(42)) levels by aggregation-induced enhanced electrochemiluminescence (ECL). Metal-organic gels (MOGs) are novel soft materials with advantages such as high gel stability, good light-emitting properties, and easy preparation. This study used silver nanoparticle metal-organic gel (Ag-MOG) as a substrate to connect A beta(42)-Ab2 and the cathodoluminescent probe. Potassium persulfate was used as a co-reactant that could emit a high ECL signal. CuS@Au had the benefits of a relatively large surface area with excellent carrier function; therefore, it was used as a substrate to load a large amount of A beta(42)-Ab1, significantly improving the immunosensor sensitivity. The ECL intensity of A beta(42) was linear in the range of 0.01 pg/mL to 250 ng/mL with a detection limit of 2.2 fg/mL (S/N = 3) under optimized detection conditions. This ECL immunosensor has been successfully applied to detect A beta(42) in human serum with the advantages of excellent stability and high selectivity. This method not only expands the potential applications of ECL immunosensors based on biological testing and clinical diagnosis but also provides a viable approach to basic clinical testing.

Automated summary

This study presents an immunosensor for measuring A beta(42) levels using aggregation-induced enhanced electrochemiluminescence. The immunosensor utilizes metal-organic gels as substrates and demonstrates high sensitivity and stability.